| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ST14 |
| Uniprot No | Q9Y5Y6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-855aa |
| 氨基酸序列 | MGSDRARKGGGGPKDFGAGLKYNSRHEKVNGLEEGVEFLPVNNVKKVEKH GPGRWVVLAAVLIGLLLVLLGIGFLVWHLQYRDVRVQKVFNGYMRITNEN FVDAYENSNSTEFVSLASKVKDALKLLYSGVPFLGPYHKESAVTAFSEGS VIAYYWSEFSIPQHLVEEAERVMAEERVVMLPPRARSLKSFVVTSVVAFP TDSKTVQRTQDNSCSFGLHARGVELMRFTTPGFPDSPYPAHARCQWALRG DADSVLSLTFRSFDLASCDERGSDLVTVYNTLSPMEPHALVQLCGTYPPS YNLTFHSSQNVLLITLITNTERRHPGFEATFFQLPRMSSCGGRLRKAQGT FNSPYYPGHYPPNIDCTWNIEVPNNQHVKVRFKFFYLLEPGVPAGTCPKD YVEINGEKYCGERSQFVVTSNSNKITVRFHSDQSYTDTGFLAEYLSYDSS DPCPGQFTCRTGRCIRKELRCDGWADCTDHSDELNCSCDAGHQFTCKNKF CKPLFWVCDSVNDCGDNSDEQGCSCPAQTFRCSNGKCLSKSQQCNGKDDC GDGSDEASCPKVNVVTCTKHTYRCLNGLCLSKGNPECDGKEDCSDGSDEK DCDCGLRSFTRQARVVGGTDADEGEWPWQVSLHALGQGHICGASLISPNW LVSAAHCYIDDRGFRYSDPTQWTAFLGLHDQSQRSAPGVQERRLKRIISH PFFNDFTFDYDIALLELEKPAEYSSMVRPICLPDASHVFPAGKAIWVTGW GHTQYGGTGALILQKGEIRVINQTTCENLLPQQITPRMMCVGFLSGGVDS CQGDSGGPLSSVEADGRIFQAGVVSWGDGCAQRNKPGVYTRLPLFRDWIK ENTGV |
| 预测分子量 | 120 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ST14重组蛋白的3条模拟参考文献示例，涵盖不同研究方向：
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1. **文献名称**: *"Recombinant ST14/matriptase蛋白的表达及其在肿瘤细胞侵袭中的功能分析"*
**作者**: 李明等
**摘要**: 本研究通过大肠杆菌系统成功表达并纯化了重组ST14蛋白，验证其丝氨酸蛋白酶活性。体外实验表明，重组ST14可促进乳腺癌细胞的迁移和侵袭，并通过激活uPA系统增强细胞外基质降解，提示其在肿瘤转移中的潜在作用。
2. **文献名称**: *"ST14重组蛋白的晶体结构解析及酶活性调控机制研究"*
**作者**: Smith J, et al.
**摘要**: 利用昆虫细胞表达系统获得高纯度ST14重组蛋白，通过X射线晶体学解析其三维结构。研究发现其催化结构域的关键氨基酸残基对底物结合至关重要，并筛选出小分子抑制剂，为靶向ST14的抗癌药物开发提供结构基础。
3. **文献名称**: *"重组ST14在皮肤屏障修复中的功能及小鼠模型验证"*
**作者**: 张华等
**摘要**: 在哺乳动物细胞中表达重组ST14蛋白，发现其通过激活PAR-2信号通路促进角质形成细胞分化。小鼠皮肤损伤模型显示，局部应用重组ST14可加速上皮再生，证实其在组织修复中的治疗潜力。
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**说明**：以上文献为模拟示例，实际研究中需通过学术数据库检索真实文献。建议使用关键词“ST14 recombinant protein”、“matriptase expression”或结合具体研究领域(如癌症、蛋白酶功能)在PubMed或Google Scholar中查找。
ST14 recombinant protein is derived from the ST14 gene, which encodes a type II transmembrane serine protease known as matriptase. This enzyme plays a critical role in epithelial development, tissue homeostasis, and inflammatory responses. Matriptase is synthesized as an inactive zymogen and undergoes autoactivation in acidic environments, a process tightly regulated by its endogenous inhibitor, hepatocyte growth factor activator inhibitor-1 (HAI-1). Dysregulation of matriptase activity has been implicated in various pathologies, including cancer metastasis, inflammatory skin disorders, and congenital ichthyosis.
The recombinant ST14 protein is typically produced using mammalian or insect expression systems to ensure proper post-translational modifications, such as glycosylation, which are essential for its enzymatic activity. Researchers utilize this recombinant protein to study protease-mediated signaling pathways, particularly those involving the activation of hepatocyte growth factor (HGF) and protease-activated receptors (PARs). It also serves as a tool for drug discovery, enabling the screening of inhibitors targeting matriptase for therapeutic applications in cancer and inflammatory diseases.
In cancer biology, elevated matriptase expression correlates with poor prognosis, as it promotes tumor invasion by degrading extracellular matrix components and activating pro-metastatic factors. Conversely, loss-of-function mutations in ST14 are linked to autosomal recessive congenital ichthyosis (ARCI), highlighting its dual role in health and disease. The recombinant protein’s availability facilitates mechanistic studies, helping to dissect its context-dependent functions and potential as a biomarker or therapeutic target.
Overall, ST14 recombinant protein is a vital resource for understanding matriptase biology and developing targeted interventions for related disorders.
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