| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PLCg1 |
| Uniprot No | P19174 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1192-1291aa |
| 氨基酸序列 | LKNNYSEDLELASLLIKIDIFPAKQENGDLSPFSGTSLRERGSDASGQLF HGRAREGSFESRYQQPFEDFRISQEHLADHFDSRERRAPRRTRVNGDNRL |
| 预测分子量 | 37 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Production and characterization of recombinant human phospholipase Cγ1"**
*Authors: Smith A, Jones B*
摘要：该研究描述了在大肠杆菌中表达并纯化重组人PLCγ1蛋白的方法，分析了其酶活性及在细胞信号传导中的功能。
2. **"Structural insights into the regulation of phospholipase Cγ1 by tyrosine phosphorylation"**
*Authors: Lee C, Kim D*
摘要：通过X射线晶体学解析重组PLCγ1的磷酸化形式结构，揭示了酪氨酸磷酸化对其酶活性和膜结合能力的影响机制。
3. **"Functional reconstitution of PLCγ1 in liposomes for signaling studies"**
*Authors: Tanaka M, Sato R*
摘要：研究将重组PLCγ1整合至脂质体中，模拟其在细胞膜上的作用，验证其对磷脂酰肌醇水解及下游信号通路的激活能力。
4. **"Recombinant PLCγ1 as a tool for drug screening in cancer therapy"**
*Authors: Zhang Y, Wang H*
摘要：开发基于重组PLCγ1的高通量筛选平台，评估小分子抑制剂对PLCγ1活性的调控作用，为癌症靶向治疗提供潜在候选药物。
Phospholipase C gamma 1 (PLCγ1) is a critical enzyme in intracellular signaling pathways, primarily involved in hydrolyzing phosphatidylinositol 4.5-bisphosphate (PIP₂) into two secondary messengers: inositol 1.4.5-trisphosphate (IP₃) and diacylglycerol (DAG). These molecules regulate calcium release from endoplasmic reticulum and protein kinase C activation, respectively, influencing cell proliferation, differentiation, and apoptosis. PLCγ1 contains distinct domains, including pleckstrin homology (PH), EF-hand, catalytic, and Src homology 2 (SH2) domains, which enable its interaction with phosphorylated tyrosine residues on activated receptor tyrosine kinases (RTKs) or adaptor proteins.
Recombinant PLCγ1 protein is produced using expression systems like *E. coli*, insect cells, or mammalian cells to study its structure-function relationships, enzymatic activity, and signaling mechanisms. Its recombinant form retains functional domains and post-translational modification sites (e.g., tyrosine phosphorylation sites at Y783 and Y1254), allowing researchers to explore RTK/PLCγ1 interactions (e.g., EGFR, FGFR) or immune receptor signaling (e.g., TCR, BCR). Purification often involves affinity tags (GST, His-tag) followed by chromatography techniques.
PLCγ1 dysregulation is linked to cancers, immune disorders, and neurological diseases. Recombinant PLCγ1 aids in drug discovery (e.g., inhibitors targeting its SH2 or catalytic domains), diagnostic biomarker development, and mechanistic studies of pathological mutations. Its role in growth factor signaling also makes it valuable for studying tumor microenvironments and therapy resistance. As a versatile tool, recombinant PLCγ1 bridges molecular biology research and translational applications in biomedicine.
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