| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GLb |
| Uniprot No | P16278 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 24-677aa |
| 氨基酸序列 | NATQRMFEIDYSRDSFLKDGQPFRYISGSIHYSRVPRFYWKDRLLKMKMA GLNAIQTYVPWNFHEPWPGQYQFSEDHDVEYFLRLAHELGLLVILRPGPY ICAEWEMGGLPAWLLEKESILLRSSDPDYLAAVDKWLGVLLPKMKPLLYQ NGGPVITVQVENEYGSYFACDFDYLRFLQKRFRHHLGDDVVLFTTDGAHK TFLKCGALQGLYTTVDFGTGSNITDAFLSQRKCEPKGPLINSEFYTGWLD HWGQPHSTIKTEAVASSLYDILARGASVNLYMFIGGTNFAYWNGANSPYA AQPTSYDYDAPLSEAGDLTEKYFALRNIIQKFEKVPEGPIPPSTPKFAYG KVTLEKLKTVGAALDILCPSGPIKSLYPLTFIQVKQHYGFVLYRTTLPQD CSNPAPLSSPLNGVHDRAYVAVDGIPQGVLERNNVITLNITGKAGATLDL LVENMGRVNYGAYINDFKGLVSNLTLSSNILTDWTIFPLDTEDAVRSHLG GWGHRDSGHHDEAWAHNSSNYTLPAFYMGNFSIPSGIPDLPQDTFIQFPG WTKGQVWINGFNLGRYWPARGPQLTLFVPQHILMTSAPNTITVLELEWAP CSSDDPELCAVTFVDRPVIGSSVTYDHPSKPVEKRLMPPPPQKNKDSWLD HVVDHHHHHH |
| 预测分子量 | 74 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与GLb重组蛋白相关的文献示例(内容为虚构示例，仅作参考)：
1. **《高效表达GLb重组蛋白的毕赤酵母系统优化研究》**
- 作者：李明等
- 摘要：研究通过毕赤酵母表达系统优化GLb重组蛋白的分泌表达，实现高产量与高纯度，并验证其抗原性可用于诊断试剂开发。
2. **《GLb重组蛋白免疫原性及佐剂效应分析》**
- 作者：王磊等
- 摘要：评估GLb重组蛋白在小鼠模型中的免疫应答，发现其联合铝佐剂可显著增强抗体滴度，提示其作为疫苗候选分子的潜力。
3. **《GLb重组蛋白结构解析与功能研究》**
- 作者：张华等
- 摘要：通过X射线晶体学解析GLb重组蛋白三维结构，揭示其与宿主细胞受体结合的关键区域，为抗病毒药物设计提供依据。
(注：实际文献需通过PubMed、Web of Science等学术平台检索关键词“GLb recombinant protein”获取。)
**Background of GLb Recombinant Protein**
Recombinant proteins, such as GLb, are engineered through genetic modification to express specific protein subunits in heterologous host systems (e.g., *E. coli*, yeast, or mammalian cells*). The GLb protein, likely derived from a viral or bacterial antigen, is designed to mimic native structural or functional epitopes for applications in vaccines, therapeutics, or diagnostic tools. For instance, in vaccine development, GLb may represent a conserved domain of a pathogen’s surface protein to elicit neutralizing antibodies.
The production of GLb involves cloning the target gene into an expression vector, followed by transfection into a host system for scalable production. Post-expression, purification techniques like affinity chromatography ensure high purity. Structural integrity is validated via methods such as SDS-PAGE, Western blot, or cryo-EM.
GLb’s design often incorporates stabilizing mutations (e.g., proline substitutions or disulfide bonds) to enhance solubility and antigenic fidelity. In the context of infectious diseases, such as COVID-19. recombinant spike proteins (similar in concept to GLb) have been pivotal in subunit vaccines (e.g., Novavax). Beyond vaccines, GLb may serve as a reagent in serological assays to detect pathogen-specific antibodies.
Challenges include achieving proper post-translational modifications (e.g., glycosylation in mammalian systems) and avoiding aggregation. Advances in structural biology and bioinformatics have optimized GLb’s design, balancing immunogenicity and manufacturability. Overall, GLb exemplifies the integration of molecular biology and immunology to address biomedical needs.
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