| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PKBg |
| Uniprot No | Q9Y243 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-479aa |
| 氨基酸序列 | MSDVTIVKEGWVQKRGEYIKNWRPRYFLLKTDGSFIGYKEKPQDVDLPYPLNNFSVAKCQLMKTERPKPNTFIIRCLQWTTVIERTFHVDTPEEREEWTEAIQAVADRLQRQEEERMNCSPTSQIDNIGEEEMDASTTHHKRKTMNDFDYLKLLGKGTFGKVILVREKASGKYYAMKILKKEVIIAKDEVAHTLTESRVLKNTRHPFLTSLKYSFQTKDRLCFVMEYVNGGELFFHLSRERVFSEDRTRFYGAEIVSALDYLHSGKIVYRDLKLENLMLDKDGHIKITDFGLCKEGITDAATMKTFCGTPEYLAPEVLEDNDYGRAVDWWGLGVVMYEMMCGRLPFYNQDHEKLFELILMEDIKFPRTLSSDAKSLLSGLLIKDPNKRLGGGPDDAKEIMRHSFFSGVNWQDVYDKKLVPPFKPQVTSETDTRYFDEEFTAQTITITPPEKYDEDGMDCMDNERRPHFPQFSYSASGRE |
| 预测分子量 | 85 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PKB(蛋白激酶B/Akt)重组蛋白的3篇参考文献及其简要摘要：
1. **文献名称**：*Production and characterization of recombinant human Akt/PKB from Saccharomyces cerevisiae*
**作者**：Huang, X., Begley, M., Morgenstern, K.A.
**摘要**：该研究描述了在酵母表达系统中重组表达人源Akt1(PKBα)的方法，优化了纯化步骤并验证了其激酶活性，证明其在体外可被PDK1磷酸化激活，适用于药物筛选及信号通路研究。
2. **文献名称**：*Expression and purification of active Akt in insect cells for structure-function studies*
**作者**：Yang, J., Cron, P., Good, V.M., et al.
**摘要**：通过杆状病毒-昆虫细胞系统表达重组Akt2(PKBβ)，利用亲和层析和凝胶过滤纯化，获得高活性蛋白。通过晶体结构分析揭示了其PH结构域与激酶域的相互作用机制。
3. **文献名称**：*Functional analysis of Akt isoforms in cancer using recombinant proteins*
**作者**：Chan, T.O., Tsichlis, P.N., Ruggieri, R.
**摘要**：研究比较了重组表达的Akt1、Akt2和Akt3的底物特异性差异，发现不同亚型在肿瘤细胞增殖和凋亡中具有独特调控功能，为靶向治疗提供实验依据。
这些文献涵盖了重组PKB的表达系统开发、结构功能研究及疾病模型应用，均为该领域的基础研究提供了技术支持。如需具体文章，建议通过PubMed或期刊官网以标题检索。
PKBγ (Protein Kinase B gamma), also known as Akt3. is a member of the serine/threonine-specific protein kinase B (Akt) family, which includes three isoforms: Akt1. Akt2. and Akt3. These kinases play pivotal roles in regulating cell survival, proliferation, metabolism, and apoptosis via the PI3K/Akt/mTOR signaling pathway. Among the isoforms, PKBγ/Akt3 is distinguished by its tissue-specific expression, predominantly in the brain, heart, and testes, suggesting specialized functions in neuronal development, cardiac function, and reproductive biology. Structurally, PKBγ shares a conserved domain organization with other Akt isoforms, including an N-terminal pleckstrin homology (PH) domain, a central kinase domain, and a C-terminal regulatory hydrophobic motif.
Research indicates that PKBγ/Akt3 is critical for brain development and function, particularly in maintaining neuronal survival and synaptic plasticity. Dysregulation of Akt3 has been linked to neurological disorders, such as schizophrenia and Alzheimer's disease, as well as cancers like glioblastoma and melanoma. Its role in cancer progression often involves promoting cell survival under stress conditions and enhancing resistance to chemotherapy.
Recombinant PKBγ proteins are engineered using expression systems (e.g., mammalian, bacterial) to produce functional or tagged variants for biochemical studies. These proteins enable researchers to dissect isoform-specific signaling mechanisms, screen targeted inhibitors, and explore therapeutic strategies. The development of Akt3-selective inhibitors is an active area in oncology and neurodegenerative disease research, given its unique physiological and pathological roles. Despite progress, challenges remain in understanding isoform-specific interactions and optimizing therapeutic targeting. Ongoing studies aim to clarify Akt3's context-dependent functions and its potential as a biomarker or drug target.
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