| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PIK3C2a |
| Uniprot No | O00443 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 全长 |
| 氨基酸序列 | full |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PIK3C2A重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**：*"PIK3C2A regulates endosomal morphogenesis and growth factor signaling through its lipid kinase activity"*
**作者**：Devereaux K. et al.
**摘要**：研究利用重组PIK3C2A蛋白揭示了其磷脂酰肌醇激酶活性对内体膜形态的调控作用，证明其通过生成PI(3)P和PI(3.4)P2脂质信号分子影响生长因子受体内吞及下游信号传导。
2. **文献名称**：*"Structural insights into the function of PIK3C2A in vesicle trafficking"*
**作者**：Wang Y. et al.
**摘要**：通过重组表达人源PIK3C2A蛋白并结合冷冻电镜技术，解析了其三维结构，发现其独特的C2结构域介导了与细胞膜的相互作用，为阐明其在囊泡运输中的分子机制提供依据。
3. **文献名称**：*"Recombinant PIK3C2A kinase domain characterization and its role in autophagy regulation"*
**作者**：Gulluni F. et al.
**摘要**：研究通过重组表达PIK3C2A激酶结构域，发现其磷酸化特定底物可调控自噬体形成，提示其在细胞自噬和代谢应激响应中的关键作用。
以上文献均聚焦于重组PIK3C2A蛋白的功能研究，涵盖脂质激酶活性、结构解析及细胞生物学机制等领域。
PIK3C2A, a member of the class II phosphatidylinositol 3-kinase (PI3K) family, is a lipid kinase that phosphorylates phosphatidylinositol (PI) to generate phosphatidylinositol 3-phosphate (PI3P). Unlike class I PI3Ks (which primarily produce PI(3.4.5)P3) and class III PI3Ks (exclusively generating PI3P), class II enzymes like PIK3C2A exhibit distinct substrate preferences and subcellular localization. The PIK3C2A protein contains a conserved lipid kinase domain, a C-terminal C2 domain for membrane interaction, and unique regulatory regions influencing its enzymatic activity and cellular functions.
This enzyme plays critical roles in membrane trafficking, vesicle formation, and intracellular signaling. It regulates endosomal dynamics, autophagy, and angiogenesis by modulating PI3P levels, which serve as docking signals for proteins containing FYVE or PX domains. PIK3C2A also participates in clathrin-coated vesicle formation and interacts with Rab GTPases to control vesicular transport. Dysregulation of PIK3C2A has been implicated in metabolic disorders, cardiovascular diseases, and cancer progression, highlighting its therapeutic potential.
Recombinant PIK3C2A proteins are engineered for functional studies, typically expressed in mammalian or insect cell systems to preserve post-translational modifications and catalytic activity. These proteins enable in vitro analysis of lipid kinase activity, protein-protein interactions, and inhibitor screening. Tagged versions (e.g., GST, His-tag) facilitate purification and detection. Research using recombinant PIK3C2A has clarified its structural-functional relationships, including how mutations affect enzymatic output and membrane binding. Current investigations focus on its role in insulin signaling, endothelial cell migration, and pathological processes like tumor angiogenesis, driving interest in developing isoform-specific PI3K modulators.
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