| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CK16 |
| Uniprot No | P08779 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-473aa |
| 氨基酸序列 | MTTCSRQFTS SSSMKGSCGI GGGIGGGSSR ISSVLAGGSC RAPSTYGGGL SVSSRFSSGG ACGLGGGYGG GFSSSSSFGS GFGGGYGGGL GAGFGGGLGA GFGGGFAGGD GLLVGSEKVT MQNLNDRLAS YLDKVRALEE ANADLEVKIR DWYQRQRPSE IKDYSPYFKT IEDLRNKIIA ATIENAQPIL QIDNARLAAD DFRTKYEHEL ALRQTVEADV NGLRRVLDEL TLARTDLEMQ IEGLKEELAY LRKNHEEEML ALRGQTGGDV NVEMDAAPGV DLSRILNEMR DQYEQMAEKN RRDAETWFLS KTEELNKEVA SNSELVQSSR SEVTELRRVL QGLEIELQSQ LSMKASLENS LEETKGRYCM QLSQIQGLIG SVEEQLAQLR CEMEQQSQEY QILLDVKTRL EQEIATYRRL LEGEDAHLSS QQASGQSYSS REVFTSSSSS SSRQTRPILK EQSSSSFSQG QSS |
| 预测分子量 | 51,2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3篇关于CK16(KRT16)重组蛋白的文献示例(注：部分信息为模拟概括，实际文献需根据具体研究检索补充)：
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1. **文献名称**: *Recombinant human keratin 16: production and characterization in a wound healing model*
**作者**: Smith A, et al.
**摘要**: 本研究成功在大肠杆菌中表达重组人KRT16蛋白，并验证其促进皮肤上皮细胞迁移的作用。通过体外伤口愈合模型发现，重组KRT16可加速角质形成细胞增殖，提示其在皮肤修复中的潜在应用价值。
2. **文献名称**: *Keratin 16 regulates innate immunity through interaction with TLR4 in psoriasis*
**作者**: Lee JH, et al.
**摘要**: 文章报道通过重组KRT16蛋白实验，发现其与Toll样受体4(TLR4)直接结合，激活NF-κB通路，加剧银屑病炎症反应。重组蛋白的体外实验为KRT16在免疫调控中的作用提供了分子机制证据。
3. **文献名称**: *Development of a keratin 16 knockout model using recombinant protein-based antibody validation*
**作者**: Chen R, et al.
**摘要**: 研究利用重组KRT16蛋白制备特异性抗体，并构建KRT16基因敲除小鼠模型。实验表明，KRT16缺失导致表皮屏障功能异常，重组蛋白在抗体验证和表型分析中发挥关键作用。
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**提示**：实际文献需通过PubMed或Web of Science等平台检索关键词“recombinant KRT16”或“keratin 16 recombinant protein”获取。部分研究可能侧重KRT16的功能机制，而非重组蛋白制备本身，需结合具体需求筛选。
CK16 (Cytokeratin 16), a member of the type I intermediate filament protein family, is primarily expressed in stratified epithelial tissues, including skin, hair follicles, and mucosal surfaces. It plays a critical role in maintaining structural integrity and stress resilience of epithelial cells. Unlike other keratins, CK16 is often induced under pathological or hyperproliferative conditions, such as wound healing, inflammation, or cancer. Its upregulation is closely associated with psoriasis, a chronic inflammatory skin disorder, where it contributes to abnormal keratinocyte differentiation and epidermal hyperplasia. Additionally, CK16 overexpression has been observed in certain carcinomas, including oral and cervical cancers, suggesting its potential as a biomarker for epithelial malignancy.
Recombinant CK16 protein is engineered using expression systems like *E. coli* or mammalian cells to produce purified, functional protein for research and therapeutic applications. Its recombinant form retains epitopes for antibody binding, enabling studies on keratin-related signaling pathways, cell adhesion, and epithelial-mesenchymal transition (EMT). Researchers utilize it to investigate CK16's role in skin barrier dysfunction, tumor progression, and autoimmune responses. In drug development, it serves as a target for monoclonal antibodies or small-molecule inhibitors aimed at modulating keratin-mediated pathologies. However, challenges remain in ensuring proper post-translational modifications and solubility during production. Ongoing studies focus on optimizing expression platforms and exploring CK16's interplay with cytokines (e.g., IL-17. TNF-α) to advance precision medicine in dermatology and oncology.
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