| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PODXL |
| Uniprot No | O00592 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 32-458aa |
| 氨基酸序列 | QNATQTTTDSSNKTAPTPASSVTIMATDTAQQSTVPTSKANEILASVKATTLGVSSDSPGTTTLAQQVSGPVNTTVARGGGSGNPTTTIESPKSTKSADTTTVATSTATAKPNTTSSQNGAEDTTNSGGKSSHSVTTDLTSTKAEHLTTPHPTSPLSPRQPTSTHPVATPTSSGHDHLMKISSSSSTVAIPGYTFTSPGMTTTLLETVFHHVSQAGLELLTSGDLPTLASQSAGITASSVISQRTQQTSSQMPASSTAPSSQETVQPTSPATALRTPTLPETMSSSPTAASTTHRYPKTPSPTVAHESNWAKCEDLETQTQSEKQLVLNLTGNTLCAGGASDEKLISLICRAVKATFNPAQDKCGIRLASVPGSQTVVVKEITIHTKLPAKDVYERLKDKWDELKEAGVSDMKLGDQGPPEEAEDRF |
| 预测分子量 | 46.2kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PODXL(Podocalyxin)重组蛋白的模拟参考文献示例(实际文献需通过数据库验证)：
1. **文献名称**: *"Recombinant Podocalyxin promotes tumor cell invasion via ERK signaling pathway"*
**作者**: Smith A, et al.
**摘要**: 研究通过大肠杆菌系统表达重组人PODXL蛋白，发现其能激活ERK信号通路，增强乳腺癌细胞的迁移和侵袭能力，提示其在肿瘤转移中的作用。
2. **文献名称**: *"Expression and purification of functional Podocalyxin extracellular domain in mammalian cells"*
**作者**: Chen L, et al.
**摘要**: 利用哺乳动物HEK293细胞成功表达带有糖基化修饰的PODXL胞外域重组蛋白，验证其与β1整合素的结合活性，为研究细胞粘附机制提供工具。
3. **文献名称**: *"Podocalyxin recombinant protein inhibits angiogenesis by blocking VEGF receptor interaction"*
**作者**: Tanaka K, et al.
**摘要**: 报道通过昆虫细胞表达系统制备重组PODXL，发现其可通过竞争性结合VEGF抑制血管内皮细胞增殖，提出其在抗血管生成治疗中的潜力。
4. **文献名称**: *"Structural analysis of Podocalyxin N-linked glycosylation using recombinant variants"*
**作者**: Müller R, et al.
**摘要**: 通过构建不同糖基化位点突变的重组PODXL蛋白，解析其糖链结构对肾小球足细胞极化的调控作用，揭示糖基化修饰的功能重要性。
**备注**：以上内容为模拟生成，实际文献需通过PubMed、Google Scholar等平台检索关键词"Podocalyxin recombinant protein"获取，并核对作者及摘要准确性。
**Background of PODXL Recombinant Protein**
Podocalyxin-like protein 1 (PODXL), a member of the CD34 family of cell surface sialomucins, is a transmembrane glycoprotein involved in cell adhesion, migration, and polarity. It is highly expressed in certain epithelial tissues, vascular endothelial cells, and hematopoietic progenitors, playing critical roles in maintaining cellular morphology and regulating intercellular interactions. Structurally, PODXL contains a heavily glycosylated extracellular domain, a single transmembrane region, and a cytoplasmic tail that interacts with cytoskeletal proteins like ezrin, linking it to the actin network.
In pathological contexts, PODXL overexpression is linked to aggressive cancers, including carcinomas of the breast, prostate, and pancreas, where it promotes metastasis by enhancing cell detachment, invasiveness, and resistance to apoptosis. Its role in cancer progression has made it a potential biomarker and therapeutic target.
Recombinant PODXL proteins are engineered *in vitro* using expression systems (e.g., mammalian, bacterial, or insect cells) to produce purified, bioactive forms of the protein. These proteins retain key functional domains, enabling researchers to study PODXL's mechanisms in cell signaling, ligand interactions, and immune modulation. They are widely used in *in vitro* assays, antibody development, and structural studies. Quality control measures, such as SDS-PAGE and mass spectrometry, ensure batch consistency and biological activity.
The availability of recombinant PODXL has advanced research into its dual roles in normal physiology and disease, offering insights for developing targeted therapies or diagnostic tools. Its study also contributes to understanding broader processes like epithelial-mesenchymal transition (EMT) and tumor microenvironment remodeling.
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