| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CD26 |
| Uniprot No | P27487 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 29-766aa |
| 氨基酸序列 | NKGTDDATADSRKTYTLTDYLKNTYRLKLYSLRWISDHEYLYKQENNILVFNAEYGNSSVFLENSTFDEFGHSINDYSISPDGQFILLEYNYVKQWRHSYTASYDIYDLNKRQLITEERIPNNTQWVTWSPVGHKLAYVWNNDIYVKIEPNLPSYRITWTGKEDIIYNGITDWVYEEEVFSAYSALWWSPNGTFLAYAQFNDTEVPLIEYSFYSDESLQYPKTVRVPYPKAGAVNPTVKFFVVNTDSLSSVTNATSIQITAPASMLIGDHYLCDVTWATQERISLQWLRRIQNYSVMDICDYDESSGRWNCLVARQHIEMSTTGWVGRFRPSEPHFTLDGNSFYKIISNEEGYRHICYFQIDKKDCTFITKGTWEVIGIEALTSDYLYYISNEYKGMPGGRNLYKIQLSDYTKVTCLSCELNPERCQYYSVSFSKEAKYYQLRCSGPGLPLYTLHSSVNDKGLRVLEDNSALDKMLQNVQMPSKKLDFIILNETKFWYQMILPPHFDKSKKYPLLLDVYAGPCSQKADTVFRLNWATYLASTENIIVASFDGRGSGYQGDKIMHAINRRLGTFEVEDQIEAARQFSKMGFVDNKRIAIWGWSYGGYVTSMVLGSGSGVFKCGIAVAPVSRWEYYDSVYTERYMGLPTPEDNLDHYRNSTVMSRAENFKQVEYLLIHGTADDNVHFQQSAQISKALVDVGVDFQAMWYTDEDHGIASSTAHQHIYTHMSHFIKQCFSLP |
| 预测分子量 | 87.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Expression and characterization of recombinant human CD26/DPP4 in mammalian cells"**
*作者：Y. Kameoka 等*
摘要：研究报道了在HEK293细胞中重组表达人CD26蛋白，分析了其酶活性及糖基化修饰对功能的影响，证实重组蛋白具有天然DPP4的催化特性。
2. **"Prokaryotic expression and purification of soluble CD26 extracellular domain for antibody screening"**
*作者：M. Tanaka 等*
摘要：通过优化大肠杆菌表达系统获得可溶性CD26胞外域重组蛋白，用于高通量筛选靶向CD26的抗体，验证其在免疫检测中的应用潜力。
3. **"Structural insights into CD26-mediated T-cell activation using recombinant protein crystallography"**
*作者：R. Zhang 等*
摘要：解析了重组CD26蛋白的晶体结构，揭示其与腺苷脱氨酶(ADA)的相互作用界面，为理解CD26在免疫信号传导中的作用提供结构基础。
4. **"Functional analysis of CD26 in glucose metabolism using recombinant DPP4-knockout mouse models"**
*作者：H. Lambeir 等*
摘要：通过重组蛋白互补实验，验证CD26/DPP4在糖尿病模型中的代谢调控功能，证明其酶活性缺失导致胰岛素敏感性变化。
CD26 recombinant protein, also known as dipeptidyl peptidase-4 (DPP4), is a multifunctional cell surface glycoprotein with enzymatic and receptor-like properties. It belongs to the serine protease family and is expressed in various tissues, including immune cells, endothelial cells, and epithelial cells. CD26 plays a critical role in immune regulation, glucose metabolism, and signal transduction by cleaving bioactive peptides such as chemokines, incretins, and neuropeptides. Its ability to truncate substrates like glucagon-like peptide-1 (GLP-1) links it to type 2 diabetes, making it a therapeutic target for drugs like sitagliptin.
Recombinant CD26 protein is engineered using mammalian or bacterial expression systems to produce soluble or membrane-bound forms. It typically retains the extracellular domain (residues 39-766 in humans), which includes the catalytic site and binding regions for interactions with adenosine deaminase (ADA), fibronectin, and caveolin-1. Structural studies reveal a homodimeric organization critical for its protease activity and immunomodulatory functions, such as T-cell activation and co-stimulation.
In research, recombinant CD26 serves as a tool to study its roles in diseases, including diabetes, cancer, autoimmune disorders, and viral infections (e.g., MERS-CoV entry via CD26 binding). It aids in drug discovery, antibody development, and mechanistic studies of its dual protease/receptor functions. Its soluble form is also explored as a biomarker for disease progression or therapeutic response. By enabling precise biochemical and functional analyses, recombinant CD26 remains pivotal in advancing therapeutic strategies and understanding its complex biology.
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