| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CSRP2 |
| Uniprot No | Q16527 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-193aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSHMPVWGG GNKCGACGRT VYHAEEVQCD GRSFHRCCFL CMVCRKNLDS TTVAIHDEEI YCKSCYGKKY GPKGYGYGQG AGTLNMDRGE RLGIKPESVQ PHRPTTNPNT SKFAQKYGGA EKCSRCGDSV YAAEKIIGAG KPWHKNCFRC AKCGKSLEST TLTEKEGEIY CKGCYAKNFG PKGFGYGQGA GALVHAQ |
| 预测分子量 | 24 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4篇关于 **CSRP2重组蛋白** 的参考文献示例(内容基于假设文献整理，具体需核实)：
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1. **标题**: *"Recombinant Human CSRP2 Protein Modulates Smooth Muscle Cell Differentiation"*
**作者**: Smith J, et al.
**摘要**: 研究利用大肠杆菌表达系统成功制备重组人源CSRP2蛋白，并证明其通过调控肌动蛋白细胞骨架重组，影响血管平滑肌细胞的分化过程。
2. **标题**: *"Expression and Functional Characterization of CSRP2 in Cardiac Development"*
**作者**: Lee S, Kim D.
**摘要**: 通过昆虫细胞系统表达重组CSRP2蛋白，发现其与心脏发育关键转录因子(如GATA4)相互作用，参与胚胎期心肌细胞增殖和形态发生。
3. **标题**: *"CSRP2 Recombinant Protein Inhibits TGF-β1-Induced Fibrosis in Vitro"*
**作者**: Wang Y, et al.
**摘要**: 利用哺乳动物细胞表达的重组CSRP2蛋白，验证其在体外抑制TGF-β1介导的成纤维细胞活化及胶原沉积，提示抗纤维化治疗潜力。
4. **标题**: *"Structural Insights into CSRP2 Protein via Recombinant Expression and Crystallography"*
**作者**: Müller R, et al.
**摘要**: 报道重组CSRP2蛋白的晶体结构解析，揭示其LIM结构域与锌离子结合的关键位点，为研究其分子互作机制提供结构基础。
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**注**：以上文献名为虚构，实际研究中建议通过 **PubMed** 或 **Google Scholar** 以关键词“CSRP2 recombinant protein”检索最新成果。
CSRP2 (Cysteine and Glycine-Rich Protein 2), also known as CRP2 or SmLIM, is a member of the CSRP family of LIM domain-containing proteins. These proteins are characterized by two tandemly repeated LIM domains, zinc-binding motifs that mediate protein-protein interactions. CSRP2 is widely expressed in vertebrates and plays regulatory roles in cellular processes such as cytoskeletal organization, cell adhesion, migration, and differentiation. It interacts with cytoskeletal components, transcription factors, and signaling molecules, influencing pathways like TGF-β and Wnt, which are critical for embryonic development and tissue homeostasis.
Research highlights CSRP2's involvement in cardiovascular and musculoskeletal development. Knockout studies in mice reveal its essential role in heart morphogenesis and smooth muscle cell function. Additionally, CSRP2 is implicated in cancer progression, where its dysregulation correlates with tumor metastasis and poor prognosis, likely through modulating epithelial-mesenchymal transition (EMT).
Recombinant CSRP2 protein is engineered using expression systems like *E. coli* or mammalian cells to produce purified, biologically active forms for experimental applications. This recombinant tool enables mechanistic studies of CSRP2's molecular interactions, structural analyses of LIM domains, and functional assays in disease models. Its use in vitro and in vivo helps elucidate CSRP2's role in developmental biology, tissue repair, and cancer, offering potential therapeutic insights. As a modular adaptor protein, CSRP2's recombinant form also serves as a scaffold in synthetic biology for designing protein interaction networks.
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