| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CSN2 |
| Uniprot No | P61201 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-443aa |
| 氨基酸序列 | MSDMEDDFMCDDEEDYDLEYSEDSNSEPNVDLENQYYNSKALKEDDPKAA LSSFQKVLELEGEKGEWGFKALKQMIKINFKLTNFPEMMNRYKQLLTYIR SAVTRNYSEKSINSILDYISTSKQMDLLQEFYETTLEALKDAKNDRLWFK TNTKLGKLYLEREEYGKLQKILRQLHQSCQTDDGEDDLKKGTQLLEIYAL EIQMYTAQKNNKKLKALYEQSLHIKSAIPHPLIMGVIRECGGKMHLREGE FEKAHTDFFEAFKNYDESGSPRRTTCLKYLVLANMLMKSGINPFDSQEAK PYKNDPEILAMTNLVSAYQNNDITEFEKILKTNHSNIMDDPFIREHIEEL LRNIRTQVLIKLIKPYTRIHIPFISKELNIDVADVESLLVQCILDNTIHG RIDQVNQLLELDHQKRGGARYTALDKWTNQLNSLNQAVVSKLA |
| 预测分子量 | 54 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CSN2重组蛋白的3篇模拟参考文献示例(注：文献内容为虚构，仅供参考)：
1. **文献名称**: "Expression and Functional Characterization of Recombinant CSN2 in Arabidopsis thaliana"
**作者**: Müller, R. et al.
**摘要**: 研究报道了拟南芥中重组CSN2蛋白的异源表达及功能分析，发现其在光形态建成中通过调控COP1活性影响植物光信号转导，为CSN复合体在泛素化途径中的作用提供新证据。
2. **文献名称**: "Crystal Structure of the CSN2 Subunit from Human COP9 Signalosome"
**作者**: Wei, L. & Zhang, Y.
**摘要**: 首次解析了人源CSN2重组蛋白的晶体结构，揭示了其PCI结构域的关键作用，并阐明其与CSN5亚基的互作界面，为靶向CSN复合体的药物设计奠定基础。
3. **文献名称**: "High-Yield Production of Recombinant CSN2 in Pichia pastoris and Its Role in DNA Damage Response"
**作者**: Tanaka, K. et al.
**摘要**: 利用毕赤酵母系统高效表达重组CSN2蛋白，证实其通过调控CRL4泛素连接酶活性参与DNA损伤修复，为癌症治疗中CSN2的功能研究提供新工具。
*注：以上文献为假设性示例，实际研究需查阅真实数据库(如PubMed)获取。*
CSN2 (β-casein) is a milk-derived phosphoprotein belonging to the casein family, which constitutes approximately 30% of total bovine milk proteins. As a naturally disordered protein, it lacks a rigid tertiary structure, enabling unique functional properties like calcium phosphate stabilization and self-assembly into micelles. These micelles serve as primary carriers of calcium and phosphorus in mammalian milk, playing critical roles in nutrient delivery and neonatal development.
Recombinant CSN2 refers to the protein produced through genetic engineering in heterologous expression systems (e.g., E. coli, yeast, or mammalian cells) rather than traditional dairy sources. This technology emerged to address limitations of animal-derived caseins, including ethical concerns about calf milk consumption, allergenicity risks, and batch-to-batch variability. The recombinant approach enables precise control over post-translational modifications, particularly phosphorylation patterns that influence mineral-binding capacity and micelle formation.
Current research focuses on optimizing expression systems to achieve proper folding and phosphorylation. Microbial systems offer cost-effectiveness but often require engineered strains for post-translational modifications, while mammalian cell cultures better preserve native protein characteristics. Applications span nutritional science (infant formula development), biomaterials (nanocarriers for drug delivery), and food technology (dairy alternatives). Recent studies explore its potential in bioactive peptide production and as a scaffold for functional nanostructures. Challenges remain in scaling production while maintaining functional equivalence to native β-casein, with ongoing work addressing yield optimization and structural fidelity.
×
