| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CSPG4 |
| Uniprot No | Q6UVK1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1583-2224aa |
| 氨基酸序列 | SLKGSQTLTVCPGSVQPLSSQTLRASSSAGTDPQLLLYRVVRGPQLGRLF HAQQDSTGEALVNFTQAEVYAGNILYEHEMPPEPFWEAHDTLELQLSSPP ARDVAATLAVAVSFEAACPQRPSHLWKNKGLWVPEGQRARITVAALDASN LLASVPSPQRSEHDVLFQVTQFPSRGQLLVSEEPLHAGQPHFLQSQLAAG QLVYAHGGGGTQQDGFHFRAHLQGPAGASVAGPQTSEAFAITVRDVNERP PQPQASVPLRLTRGSRAPISRAQLSVVDPDSAPGEIEYEVQRAPHNGFLS LVGGGLGPVTRFTQADVDSGRLAFVANGSSVAGIFQLSMSDGASPPLPMS LAVDILPSAIEVQLRAPLEVPQALGRSSLSQQQLRVVSDREEPEAAYRLI QGPQYGHLLVGGRPTSAFSQFQIDQGEVVFAFTNFSSSHDHFRVLALARG VNASAVVNVTVRALLHVWAGGPWPQGATLRLDPTVLDAGELANRTGSVPR FRLLEGPRHGRVVRVPRARTEPGGSQLVEQFTQQDLEDGRLGLEVGRPEG RAPGPAGDSLTLELWAQGVPPAVASLDFATEPYNAARPYSVALLSVPEAA RTEAGKPESSTPTGEPGPMASSPEPAVAKGGFLSFLEANMFS |
| 预测分子量 | 73 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CSPG4重组蛋白的3篇代表性文献示例(注：文献为模拟示例，实际文献需通过数据库验证)：
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1. **文献名称**: *"CSPG4-specific CAR-T cells exhibit antitumor activity against solid tumors"*
**作者**: Marvin, J. et al. (2019)
**摘要**: 研究利用重组CSPG4蛋白体外筛选并优化靶向CSPG4的CAR-T细胞，证明其在黑色素瘤和胶质瘤异种移植模型中显著抑制肿瘤生长，提示CSPG4重组蛋白在肿瘤免疫治疗中的应用潜力。
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2. **文献名称**: *"Structural characterization of the CSPG4 extracellular domain reveals novel binding motifs"*
**作者**: Smith, R. & Patel, K. (2020)
**摘要**: 通过表达并纯化重组CSPG4胞外域蛋白，结合X射线晶体学解析其三维结构，发现其与整合素和生长因子受体相互作用的特异性结构域，为开发靶向抑制剂提供依据。
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3. **文献名称**: *"CSPG4 recombinant protein promotes tumor angiogenesis via integrin αvβ3 signaling"*
**作者**: Liu, Y. et al. (2021)
**摘要**: 研究发现重组CSPG4蛋白通过激活整合素αvβ3信号通路增强内皮细胞迁移和血管生成，揭示其在肿瘤微环境中的促血管生成机制，为抗血管治疗提供新靶点。
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**建议**：实际研究中可参考PubMed、Web of Science等平台，以关键词“CSPG4 recombinant protein”或“NG2 recombinant protein”检索最新文献，重点关注其在肿瘤靶向治疗、信号通路或结构生物学领域的研究。
Chondroitin sulfate proteoglycan 4 (CSPG4), also known as neuron-glial antigen 2 (NG2) or melanoma-associated chondroitin sulfate proteoglycan (MCSP), is a transmembrane proteoglycan characterized by a core protein decorated with chondroitin sulfate glycosaminoglycan chains. It is expressed on the cell surface of various cell types, including neural glial progenitor cells, pericytes, chondrocytes, and certain cancer cells, particularly aggressive melanoma. CSPG4 plays critical roles in cellular adhesion, migration, proliferation, and signaling by interacting with extracellular matrix components and growth factors like PDGF-AA and FGF2. Its involvement in tumor progression, angiogenesis, and metastasis has made it a therapeutic target in oncology.
In the nervous system, CSPG4/NG2 is a marker for oligodendrocyte precursor cells (OPCs), regulating myelination and repair processes. Dysregulation of CSPG4 is associated with neurodegenerative diseases and brain injury. Recombinant CSPG4 protein, produced through genetic engineering in bacterial or mammalian expression systems, retains functional domains for studying its molecular interactions, signaling pathways, and pathological contributions. Researchers utilize it to develop monoclonal antibodies, CAR-T cells, or small-molecule inhibitors targeting CSPG4-overexpressing cancers. Additionally, it serves as a tool to explore tissue regeneration mechanisms or tumor microenvironment dynamics. Its versatility in both basic research and translational applications underscores CSPG4's significance in biomedicine.
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