| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CST1 |
| Uniprot No | P01037 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 21-141aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMWSPKEEDRIIPGGIYNADLNDEWVQR ALHFAISEYNKATKDDYYRRPLRVLRARQQTVGGVNYFFDVEVGRTICTK SQPNLDTCAFHEQPELQKKQLCSFEIYEVPWENRRSLVKS RCQES |
| 预测分子量 | 17 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CST1(Cystatin-SN)重组蛋白的3篇文献示例(注：文献信息为示例性质，实际引用请核实原文)：
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1. **标题**: *"Recombinant human CST1 protein inhibits cysteine proteases and modulates inflammatory response in oral epithelial cells"*
**作者**: Zhang L, et al.
**摘要**: 本研究通过大肠杆菌系统表达并纯化了重组人CST1蛋白，验证其抑制半胱氨酸蛋白酶(如组织蛋白酶B)的活性，并发现其通过调节NF-κB通路减轻口腔上皮细胞的炎症反应，提示CST1可能在口腔黏膜免疫中发挥作用。
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2. **标题**: *"Expression and functional characterization of CST1 recombinant protein in gastric cancer progression"*
**作者**: Wang Y, et al.
**摘要**: 研究利用哺乳动物细胞表达系统制备CST1重组蛋白，发现其通过抑制肿瘤微环境中的蛋白酶活性，降低胃癌细胞侵袭能力，并可能作为潜在生物标志物用于胃癌诊断或治疗靶点。
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3. **标题**: *"Optimization of CST1 recombinant protein production in Pichia pastoris and its application in allergy research"*
**作者**: Kim S, et al.
**摘要**: 文章报道了在毕赤酵母中高效表达CST1重组蛋白的工艺优化，证明重组蛋白能特异性结合IgE抗体，可能参与过敏性哮喘的病理过程，为开发过敏原诊断工具提供实验基础。
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**备注**：以上文献为示例，实际研究中请通过PubMed、Web of Science等平台检索最新文献。若需具体文献，建议补充CST1研究领域(如免疫学、癌症或口腔生物学)以便精准推荐。
Cystatin SN (CST1) is a member of the cystatin superfamily, a group of cysteine protease inhibitors that regulate proteolytic activity by binding to cathepsins and other cysteine proteases. Encoded by the CST1 gene in humans, it is classified as a type 2 cystatin, secreted primarily by salivary glands and mucosal tissues. CST1 plays a role in innate immunity, maintaining tissue homeostasis by controlling protease-mediated processes such as inflammation, protein degradation, and pathogen defense. Dysregulation of CST1 expression has been linked to oral diseases, cancers, and autoimmune conditions, highlighting its biological significance.
Recombinant CST1 protein is engineered using expression systems (e.g., Escherichia coli or mammalian cells) to produce purified, functional protein for research and therapeutic applications. Its production involves cloning the CST1 gene into expression vectors, followed by fermentation, purification via affinity chromatography, and quality validation (e.g., SDS-PAGE, Western blot). Recombinant CST1 retains the ability to inhibit target proteases, making it valuable for studying protease-related pathways, developing diagnostic biomarkers (e.g., for oral squamous cell carcinoma), or exploring therapeutic strategies in inflammatory disorders. Recent studies also suggest its potential as a mucosal protective agent or in modulating immune responses. As a well-characterized reagent, recombinant CST1 accelerates mechanistic studies and translational research in immunology, oncology, and molecular biology.
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