| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CST11 |
| Uniprot No | Q9H112-2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 26-103aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSARKKTFL SVHEVMAVEN YAKDSLQWIT DQYNKESDDK YHFRIFRVLK VQRQQVNCFF SVFAVPWFEQ YKILNKSCSS D |
| 预测分子量 | 12 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CST11重组蛋白的模拟参考文献示例(请注意，这些文献为虚构示例，实际研究请通过学术数据库查询)：
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1. **标题**：*"Recombinant CST11 Protein Inhibits Protease Activity in Sperm Maturation"*
**作者**：Smith A, et al.
**摘要**：本研究通过在大肠杆菌中表达重组CST11蛋白，验证了其在男性生殖系统中对精子成熟相关蛋白酶(如cathepsin)的抑制作用，揭示了CST11在生殖调控中的潜在功能。
2. **标题**：*"Structural Characterization and Functional Analysis of CST11 in Immune Regulation"*
**作者**：Li X, et al.
**摘要**：利用哺乳动物细胞系统表达CST11重组蛋白，解析其三维结构，并发现其通过调控T细胞活性参与免疫应答，为自身免疫疾病治疗提供新靶点。
3. **标题**：*"Optimization of CST11 Recombinant Expression in Yeast and Its Antifungal Activity"*
**作者**：Wang Y, et al.
**摘要**：在毕赤酵母中优化CST11重组蛋白表达工艺，证明其对病原真菌(如白色念珠菌)的抑制作用，为开发新型抗真菌药物奠定基础。
4. **标题**：*"CST11 Recombinant Protein Attenuates Tumor Metastasis via Matrix Metalloproteinase Inhibition"*
**作者**：Chen Z, et al.
**摘要**：通过体外实验证实，重组CST11蛋白可显著抑制肿瘤细胞侵袭，机制涉及对MMP-2/9活性的调控，提示其在癌症治疗中的应用潜力。
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建议通过PubMed、Web of Science或Google Scholar检索关键词(如"CST11 recombinant protein"、"Cystatin 11 expression")获取真实文献。
**Background of CST11 Recombinant Protein**
CST11 (Cystatin 11) is a member of the cystatin superfamily, a group of cysteine protease inhibitors that regulate proteolytic enzymes like cathepsins. These proteins play critical roles in maintaining cellular homeostasis, immune response modulation, and tissue remodeling. CST11 is primarily expressed in reproductive tissues, notably in the epididymis, suggesting its involvement in sperm maturation and fertility. Its inhibitory activity targets proteases that could otherwise degrade extracellular matrices or disrupt cellular integrity, highlighting its potential role in protecting spermatozoa during their transit and storage.
Recombinant CST11 is engineered using biotechnological methods, typically through expression in bacterial (e.g., *E. coli*) or mammalian cell systems. This allows large-scale production of the protein with high purity and consistency. The recombinant form retains the functional properties of native CST11. including its ability to bind and inhibit target proteases. Structural studies reveal conserved cystatin-like domains, such as a protease-binding epitope and a conserved N-terminal region critical for inhibitory activity.
Research on CST11 has focused on its implications in male fertility, with studies linking its dysregulation to reproductive disorders. Additionally, its anti-proteolytic properties are being explored in biomedical contexts, such as inflammation, cancer metastasis, and neurodegenerative diseases, where uncontrolled protease activity contributes to pathology. Recombinant CST11 serves as a valuable tool for mechanistic studies, diagnostic assays, and therapeutic development. Recent advances in protein engineering have further optimized its stability and bioavailability, enhancing its potential for clinical translation.
In summary, CST11 recombinant protein bridges fundamental reproductive biology and broader biomedical applications, offering insights into protease regulation and therapeutic innovation.
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