| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CD42d |
| Uniprot No | P40197 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-560aa |
| 氨基酸序列 | MLRGTLLCAVLGLLRAQPFPCPPACKCVFRDAAQCSGGDVARISALGLPTNLTHILLFGMGRGVLQSQSFSGMTVLQRLMISDSHISAVAPGTFSDLIKLKTLRLSRNKITHLPGALLDKMVLLEQLFLDHNALRGIDQNMFQKLVNLQELALNQNQLDFLPASLFTNLENLKLLDLSGNNLTHLPKGLLGAQAKLERLLLHSNRLVSLDSGLLNSLGALTELQFHRNHIRSIAPGAFDRLPNLSSLTLSRNHLAFLPSALFLHSHNLTLLTLFENPLAELPGVLFGEMGGLQELWLNRTQLRTLPAAAFRNLSRLRYLGVTLSPRLSALPQGAFQGLGELQVLALHSNGLTALPDGLLRGLGKLRQVSLRRNRLRALPRALFRNLSSLESVQLDHNQLETLPGDVFGALPRLTEVLLGHNSWRCDCGLGPFLGWLRQHLGLVGGEEPPRCAGPGAHAGLPLWALPGGDAECPGPRGPPPRPAADSSSEAPVHPALAPNSSEPWVWAQPVTTGKGQDHSPFWGFYFLLLAVQAMITVIIVFAMIKIGQLFRKLIRERALG |
| 预测分子量 | 60,9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CD42d(GPIBB)重组蛋白的3篇参考文献示例(注：文献为示例性概括，实际文献需根据具体研究补充)：
1. **文献名称**：*"Recombinant expression and functional characterization of human CD42d in platelet adhesion"*
**作者**：Chen L, et al.
**摘要**：研究通过哺乳动物细胞系统成功表达功能性重组CD42d蛋白，证实其与vWF(血管性血友病因子)的相互作用，并证明其在血小板粘附中的关键作用，为血栓性疾病机制提供新见解。
2. **文献名称**：*"Structural analysis of GPIBB-CD42d in the GPIb-IX-V complex via recombinant co-expression"*
**作者**：Zhang Y, et al.
**摘要**：通过共表达CD42d与其他亚基(GPIbα、GPIX)，解析了GPIb-IX-V复合体的组装机制，发现CD42d对复合体稳定性至关重要，并利用冷冻电镜揭示了其跨膜结构域的关键相互作用。
3. **文献名称**：*"A recombinant CD42d-based peptide inhibits arterial thrombosis in murine models"*
**作者**：Wang H, et al.
**摘要**：开发了一种基于CD42d胞外域的重组融合蛋白，可竞争性阻断vWF与血小板的结合，在动物模型中显著减少血栓形成，提示其作为抗血栓治疗药物的潜力。
(注：以上为模拟文献，实际引用需查询具体数据库如PubMed/NCBI。)
CD42d, also known as glycoprotein Ib beta (GPIBβ), is a subunit of the platelet surface glycoprotein Ib-IX-V (GPIb-IX-V) complex, a critical receptor involved in hemostasis and thrombosis. This transmembrane protein, encoded by the GPIBB gene, forms a disulfide-linked complex with GPIbα (CD42b) and non-covalently associates with GPIX (CD42a) and GPV (CD42d) to mediate platelet adhesion under shear stress. The GPIb-IX-V complex primarily binds von Willebrand factor (vWF) at sites of vascular injury, initiating platelet activation and aggregation. CD42d plays a structural role in stabilizing the receptor complex and participates in intracellular signaling.
Recombinant CD42d proteins are engineered using expression systems like mammalian CHO cells to ensure proper post-translational modifications. These proteins retain functional domains, including leucine-rich repeats and cysteine-rich regions, crucial for maintaining structural integrity and ligand-binding capabilities. Researchers utilize recombinant CD42d to study platelet biology, inherited bleeding disorders (e.g., Bernard-Soulier syndrome linked to GPIBB mutations), and thrombotic mechanisms. Its applications extend to drug discovery, particularly in developing antiplatelet therapies targeting vWF-GPIb interactions. Additionally, recombinant CD42d serves as a critical reagent in diagnostic assays for platelet function testing. Structural studies using this protein have advanced understanding of GPIb-IX-V complex dynamics and its role in mechanotransduction during thrombus formation. By eliminating confounding factors in whole-blood systems, recombinant CD42d provides a controlled platform for investigating molecular interactions and signaling pathways in cardiovascular diseases.
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