| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CD64 |
| Uniprot No | P12314 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-374aa |
| 氨基酸序列 | MWFLTTLLLWVPVDGQVDTTKAVITLQPPWVSVFQEETVTLHCEVLHLPG SSSTQWFLNGTATQTSTPSYRITSASVNDSGEYRCQRGLSGRSDPIQLEI HRGWLLLQVSSRVFTEGEPLALRCHAWKDKLVYNVLYYRNGKAFKFFHWN SNLTILKTNISHNGTYHCSGMGKHRYTSAGISVTVKELFPAPVLNASVTS PLLEGNLVTLSCETKLLLQRPGLQLYFSFYMGSKTLRGRNTSSEYQILTA RREDSGLYWCEAATEDGNVLKRSPELELQVLGLQLPTPVWFHVLFYLAVG IMFLVNTVLWVTIRKELKRKKKWDLEISLDSGHEKKVISSLQEDRHLEEE LKCQEQKEEQLQEGVHRKEPQGAT |
| 预测分子量 | 67 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CD64(FcγRI)重组蛋白研究的虚构参考文献示例(仅供示例参考，非真实文献)：
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1. **文献名称**：*Expression and Purification of Recombinant Human CD64 in Escherichia coli*
**作者**：Smith A, et al.
**摘要**：研究报道了通过原核表达系统(大肠杆菌)高效表达重组人CD64蛋白的优化方法，并利用亲和层析技术纯化获得高纯度蛋白，验证了其与IgG的结合活性。
2. **文献名称**：*Structural Analysis of CD64-Fc Complex by X-ray Crystallography*
**作者**：Zhang L, et al.
**摘要**：通过X射线晶体学解析了重组CD64蛋白与IgG Fc段的复合物结构，揭示了CD64的抗原结合域关键氨基酸残基及其相互作用机制。
3. **文献名称**：*CD64 as a Biomarker in Sepsis: Recombinant Protein-based Diagnostic Assay Development*
**作者**：Johnson R, et al.
**摘要**：利用重组CD64蛋白开发了一种新型ELISA检测方法，用于快速诊断脓毒症患者外周血中CD64表达水平，证明其与疾病严重程度的相关性。
4. **文献名称**：*Targeted Drug Delivery Using CD64-Fusion Protein Conjugates*
**作者**：Garcia M, et al.
**摘要**：构建了重组CD64-抗体融合蛋白，验证其在体外模型中特异性结合肿瘤细胞表面抗原的能力，为靶向递送抗癌药物提供了新策略。
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**注意**：以上文献为虚构示例，实际研究中建议通过PubMed、Web of Science或Google Scholar等平台检索关键词(如“CD64 recombinant protein”、“FcγRI expression”)获取真实文献。
CD64. also known as Fcγ receptor I (FcγRI), is a high-affinity receptor for the Fc region of immunoglobulin G (IgG). It belongs to the immunoglobulin superfamily and is primarily expressed on myeloid cells, including monocytes, macrophages, and dendritic cells. As a key player in immune responses, CD64 facilitates pathogen recognition, antibody-dependent cellular phagocytosis (ADCP), and immune complex clearance by binding to IgG-opsonized targets. Its expression is upregulated during infections or inflammatory conditions, making it a potential biomarker for sepsis and other immune-related disorders.
Recombinant CD64 protein is engineered using molecular cloning techniques, typically produced in mammalian or insect cell systems to ensure proper glycosylation and functional fidelity. The recombinant form often includes the extracellular domain of CD64. which retains IgG-binding capacity, and may be fused with tags (e.g., Fc or fluorescent tags) for detection and purification. This engineered protein serves as a critical tool for studying immune cell interactions, IgG-mediated signaling, and Fc receptor biology in vitro.
Applications span therapeutic and diagnostic development. In research, recombinant CD64 aids in elucidating mechanisms of autoimmune diseases, infectious immunity, and cancer immunotherapy. Clinically, it has been explored to enhance antibody-dependent cellular cytotoxicity (ADCC) in cancer treatments or to neutralize pathogenic immune complexes. Recent studies also investigate its role in modulating immune responses through decoy receptors or targeted drug delivery systems. Its versatility underscores its importance in both basic immunology and translational medicine.
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