| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PLA2G4 |
| Uniprot No | P47712 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-749aa |
| 氨基酸序列 | MSFIDPYQHIIVEHQYSHKFTVVVLRATKVTKGAFGDMLDTPDPYVELFI STTPDSRKRTRHFNNDINPVWNETFEFILDPNQENVLEITLMDANYVMDE TLGTATFTVSSMKVGEKKE VPFIFNQVTEMVLEMSLEVCSCPDLRFSM ALCDQEKTFRQQRKEHIRESMKKLLGPKN SEGLHSARDVPVVAILGSG GGFRAMVGFSGVMKALYESGILDCATYVAGLSGSTWYM STLYSHPDFP EKGPEEINEELMKNVSHNPLLLLTPQKVKRYVESLWKKKSSGQPVTFTD IFGMLIGETLIHNRMNTTLSSLKEKVNTAQCPLPLFTCLHVKPDVSELM FADWVEFSPYE IGMAKYGTFMAPDLFGSKFFMGTVVKKYEENPLHFLM GVWGSAFSILFNRVLGVSGSQ SRGSTMEEELENITTKHIVSNDSSDSD DESHEPKGTENEDAGSDYQSDNQASWIHRMI MALVSDSALFNTREGRA GKVHNFMLGLNLNTSYPLSPLSDFATQDSFDDDELDAAVAD PDEFERI YEPLDVKSKKIHVVDSGLTFNLPYPLILRPQRGVDLIISFDFSARPSDSS PPFK ELLLAEKWAKMNKLPFPKIDPYVFDREGLKECYVFKPKNPDMEK DCPTIIHFVLANINFR KYKAPGVPRETEEEKEIADFDIFDDPESPFST FNFQYPNQAFKRLHDLMHFNTLNNIDVI KEAMVESIEYRRQNPSRCSV SLSNVEARRFFNKEFLSKPKADYKDDDDKHHHHHH |
| 预测分子量 | 87 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PLA2G4重组蛋白的3-4篇文献示例(内容基于学术知识综合概括)：
---
1. **文献名称**：*"Cloning and expression of a human cDNA encoding calcium-dependent phospholipase A2 (cPLA2)"*
**作者**：Clark JD, Lin LL, Kriz RW, et al.
**摘要**：该研究首次成功克隆了人源PLA2G4A基因，并在哺乳动物细胞中表达了重组cPLA2α蛋白，证实其具有钙依赖性催化活性，能够选择性水解膜磷脂释放花生四烯酸，为后续研究炎症信号通路奠定基础。
2. **文献名称**：*"Crystal structure of human cytosolic phospholipase A2 reveals a novel topology and catalytic mechanism"*
**作者**：Dessen A, Tang J, Schmidt H, et al.
**摘要**：通过X射线晶体学解析了重组cPLA2α的三维结构，揭示了其独特的催化域和钙离子结合位点，阐明了该酶通过“脂质界面吸附”机制识别底物的分子基础。
3. **文献名称**：*"Role of cytosolic phospholipase A2 in allergic response and parturition"*
**作者**：Uozumi N, Kume K, Nagase T, et al.
**摘要**：利用重组cPLA2α蛋白及基因敲除小鼠模型，证明该酶在过敏反应中通过生成类二十烷酸类炎症介质驱动病理过程，并发现其缺失导致小鼠分娩障碍，提示生理与病理双重作用。
4. **文献名称**：*"High-throughput screening of cPLA2α inhibitors using recombinant protein and fluorescence-based assay"*
**作者**：Reinhardt SM, Chen Y, Dennis EA.
**摘要**：开发基于重组cPLA2α蛋白的高通量荧光活性检测方法，筛选出多个新型抑制剂，为抗炎药物研发提供先导化合物。
---
注：以上文献为示例性内容，实际引用时需核实原文信息。
**Background of PLA2G4 Recombinant Protein**
Phospholipase A2 Group IV (PLA2G4), also known as cytosolic phospholipase A2-alpha (cPLA2α), is a calcium-dependent enzyme encoded by the *PLA2G4A* gene. It plays a pivotal role in inflammation and immune responses by catalyzing the hydrolysis of membrane phospholipids to release arachidonic acid (AA), a precursor for pro-inflammatory lipid mediators such as prostaglandins, leukotrienes, and thromboxanes. This enzyme is distinguished from other phospholipases by its specificity for sn-2 acyl bonds in glycerophospholipids and its regulation via phosphorylation and calcium signaling.
Structurally, PLA2G4 contains a catalytic domain with conserved Ser-Asp dyad residues critical for enzymatic activity, as well as a calcium-dependent lipid-binding (C2) domain that mediates its translocation to membranes upon intracellular calcium elevation. Dysregulation of PLA2G4 is implicated in numerous pathological conditions, including chronic inflammation, cardiovascular diseases, cancer, and neurodegenerative disorders. Its role in amplifying inflammatory cascades has made it a target for therapeutic intervention.
Recombinant PLA2G4 protein is produced using expression systems like mammalian cells (e.g., HEK293 or CHO) to ensure proper post-translational modifications and functional fidelity. It serves as a vital tool for studying enzyme kinetics, inhibitor screening, and elucidating mechanisms underlying inflammation-related diseases. Purification typically involves affinity chromatography, with quality assessments via SDS-PAGE, Western blot, and activity assays. Research utilizing PLA2G4 recombinant protein has advanced the development of selective inhibitors, offering potential therapies for conditions driven by excessive phospholipase activity.
In summary, PLA2G4 recombinant protein bridges biochemical research and therapeutic innovation, providing insights into lipid-mediated signaling and inflammation while supporting drug discovery efforts.
×
