| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CASP1 |
| Uniprot No | P29466 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-91aa |
| 氨基酸序列 | MADKVLKEKRKLFIRSMGEGTINGLLDELLQTRVLNKEEMEKVKRENATVMDKTRALIDSVIPKGAQACQICITYICEEDSYLAGTLGLSA |
| 预测分子量 | 11.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CASP1重组蛋白的3篇参考文献示例(注：文献信息为虚构示例，实际引用请以真实文献为准)：
1. **"Expression and Functional Characterization of Recombinant Caspase-1 in Escherichia coli"**
*作者：Smith A, et al.*
摘要：本研究利用大肠杆菌系统成功表达并纯化了重组CASP1蛋白，通过体外酶活实验证实其可特异性切割IL-1β前体，为炎症小体功能研究提供工具。
2. **"Structural Insights into Caspase-1 Activation by Recombinant Protein Crystallography"**
*作者：Zhang L, et al.*
摘要：通过解析重组CASP1的晶体结构，揭示了其活性位点构象变化及抑制剂结合机制，为靶向CASP1的药物设计提供结构基础。
3. **"Role of Recombinant Caspase-1 in Pyroptosis and Neuroinflammation Models"**
*作者：Johnson R, et al.*
摘要：利用重组CASP1蛋白验证其在神经元焦亡中的关键作用，并证明其抑制剂可减轻体外炎症反应，提示治疗神经退行性疾病的潜力。
4. **"Optimization of Caspase-1 Recombinant Production for High-Throughput Drug Screening"**
*作者：Wang Y, et al.*
摘要：开发了昆虫细胞表达系统的高效CASP1重组蛋白制备方案，应用于大规模化合物筛选，成功鉴定出新型CASP1小分子抑制剂。
(提示：实际研究中建议通过PubMed或Web of Science检索关键词“CASP1 recombinant”获取权威文献。)
Caspase-1 (CASP1), also known as interleukin-1β converting enzyme (ICE), is a cysteine-aspartic protease that plays a central role in inflammasome-mediated inflammatory responses and pyroptosis. It is synthesized as an inactive zymogen (pro-CASP1) and undergoes proteolytic activation upon sensing pathogenic or stress signals, typically through inflammasome complexes like NLRP3. Activated CASP1 cleaves pro-inflammatory cytokines pro-IL-1β and pro-IL-18 into their mature forms, which drive systemic inflammation. Additionally, it induces pyroptosis by cleaving gasdermin D, promoting membrane pore formation and inflammatory cell death.
Recombinant CASP1 proteins are engineered for research and therapeutic applications. Produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), these proteins retain enzymatic activity and are used to study inflammasome signaling, cytokine maturation, and cell death mechanisms *in vitro*. Structural studies of recombinant CASP1 have revealed its domain organization, including a CARD domain, p20/p10 catalytic subunits, and regulatory cleavage sites. Researchers also employ it to screen inhibitors targeting autoimmune or inflammatory diseases, such as rheumatoid arthritis or sepsis. However, its uncontrolled activation is linked to pathological inflammation, making it a double-edged target. Recombinant CASP1 tools continue to advance our understanding of innate immunity and guide drug development for inflammation-related disorders.
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