| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CD146 |
| Uniprot No | P43121 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-646aa |
| 氨基酸序列 | MGLPRLVCAFLLAACCCCPRVAGVPGEAEQPAPELVEVEVGSTALLKCGL SQSQGNLSHVDWFSVHKEKRTLIFRVRQGQGQSEPGEYEQRLSLQDRGAT LALTQVTPQDERIFLCQGKRPRSQEYRIQLRVYKAPEEPNIQVNPLGIPV NSKEPEEVATCVGRNGYPIPQVIWYKNGRPLKEEKNRVHIQSSQTVESSG LYTLQSILKAQLVKEDKDAQFYCELNYRLPSGNHMKESREVTVPVFYPTE KVWLEVEPVGMLKEGDRVEIRCLADGNPPPHFSISKQNPSTREAEEETTN DNGVLVLEPARKEHSGRYECQGLDLDTMISLLSEPQELLVNYVSDVRVSP AAPERQEGSSLTLTCEAESSQDLEFQWLREETGQVLERGPVLQLHDLKRE AGGGYRCVASVPSIPGLNRTQLVNVAIFGPPWMAFKERKVWVKENMVLNL SCEASGHPRPTISWNVNGTASEQDQDPQRVLSTLNVLVTPELLETGVECT ASNDLGKNTSILFLELVNLTTLTPDSNTTTGLSTSTASPHTRANSTSTER KLPEPESRGVVIVAVIVCILVLAVLGAVLYFLYKKGKLPCRRSGKQEITL PPSRKSELVVEVKSDKLPEEMGLLQGSSGDKRAPGDQGEKYIDLRH |
| 预测分子量 | 97 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3篇与CD146重组蛋白相关的参考文献示例(内容为概括性描述，非真实文献)：
1. **"Recombinant CD146 protein promotes angiogenesis through VEGFR2 signaling pathway"**
*Zhang L, et al. (2015)*
摘要：研究通过大肠杆菌系统表达人源CD146重组蛋白，发现其能够激活血管内皮细胞中的VEGFR2通路，促进体外血管生成，提示其在缺血性疾病治疗中的潜在应用。
2. **"Structural characterization of soluble CD146 and its interaction with inflammatory cytokines"**
*Metaxa E, et al. (2018)*
摘要：利用哺乳动物细胞系成功纯化可溶性CD146重组蛋白，通过X射线晶体学解析其三维结构，并证明其通过与IL-6和TNF-α结合调控炎症反应。
3. **"CD146 recombinant protein inhibits melanoma metastasis by blocking EMT process"**
*Wang Y, et al. (2020)*
摘要：构建CD146-Fc融合蛋白，实验显示其通过抑制上皮-间质转化(EMT)关键分子如Snail和Twist的表达，显著降低黑色素瘤细胞的迁移和侵袭能力。
(注：以上为模拟文献，如需真实文献建议通过PubMed或Web of Science以"CD146 recombinant protein"为关键词检索最新研究)
CD146. also known as Melanoma Cell Adhesion Molecule (MCAM) or MUC18. is a transmembrane glycoprotein belonging to the immunoglobulin superfamily. Initially identified as a marker for melanoma progression, it is now recognized as a multifunctional receptor expressed on endothelial cells, smooth muscle cells, immune cells, and various cancer cells. Its structure includes five extracellular immunoglobulin-like domains, a transmembrane region, and a short cytoplasmic tail. CD146 plays critical roles in cell adhesion, migration, angiogenesis, and immune regulation, mediated through interactions with ligands like laminin, Wnt proteins, or integrins.
Recombinant CD146 proteins are engineered using expression systems (e.g., HEK293 or CHO cells) to produce soluble extracellular domains or full-length forms for research and therapeutic applications. These proteins retain functional epitopes, enabling studies on CD146’s signaling mechanisms, particularly in pathological contexts. For example, CD146 overexpression correlates with tumor metastasis by promoting EMT (epithelial-mesenchymal transition) and vascular remodeling. In vascular biology, it regulates endothelial permeability and leukocyte trafficking via NF-κB and MAPK pathways. Immunologically, CD146 modulates T-cell activation and macrophage polarization, linking it to inflammatory diseases.
Therapeutically, recombinant CD146 serves as a tool to develop neutralizing antibodies or decoy receptors targeting conditions like cancer, atherosclerosis, or autoimmune disorders. Recent studies explore its diagnostic potential as a soluble biomarker in serum for disease progression monitoring. Challenges remain in elucidating its dual roles (pro- or anti-inflammatory depending on context) and optimizing clinical-grade recombinant forms. Overall, CD146 recombinant proteins bridge mechanistic research and translational innovation in vascular and oncological therapeutics.
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