| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CK10 |
| Uniprot No | P13645 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 345-454aa |
| 氨基酸序列 | KELTTEIDNNIEQISSYKSEITELRRNVQALEIELQSQLALKQSLEASLA ETEGRYCVQLSQIQAQISALEEQLQ QIRAETECQNTEYQQLLDIKIRL ENEIQTYRSLLE |
| 预测分子量 | 38 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CK10重组蛋白的3篇代表性文献概览：
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1. **文献名称**：*Recombinant Human Keratin 10: Production and Characterization in Escherichia coli*
**作者**：Smith J, et al.
**摘要**：本研究报道了在大肠杆菌中高效表达重组人CK10蛋白的方法，通过优化密码子与纯化步骤获得高纯度蛋白。实验证实重组CK10具有天然蛋白的免疫反应性，可用于单克隆抗体的开发及皮肤疾病的体外研究。
2. **文献名称**：*Structural Analysis of Keratin 10 in Epidermal Differentiation Using Recombinant Protein Models*
**作者**：Chen L, Wang H.
**摘要**：作者利用杆状病毒表达系统制备重组CK10蛋白，结合X射线晶体学解析其结构，揭示了其螺旋结构域在表皮细胞分化中的关键作用，为遗传性角化异常疾病机制提供了分子层面的解释。
3. **文献名称**：*CK10 Recombinant Protein as a Biomarker for Psoriasis Severity Assessment*
**作者**：Gupta R, et al.
**摘要**：该研究通过哺乳动物细胞表达系统获得功能性CK10重组蛋白，验证其与银屑病患者血清抗体的特异性结合，提出其作为疾病活动度评估的新型生物标志物的潜力。
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**说明**：上述文献为模拟概括，实际研究中建议通过PubMed或Web of Science以“recombinant keratin 10”等关键词检索最新论文，并优先选择高影响力期刊(如*Journal of Investigative Dermatology*)或权威蛋白研究期刊的文献。
CK10 recombinant protein, derived from the human cytokeratin 10 (KRT10) gene, is a key structural protein in epithelial cells. Cytokeratins are intermediate filament proteins that form the cytoskeleton, providing mechanical stability and regulatory functions in epithelial tissues. CK10 is specifically expressed in the suprabasal layers of stratified epithelia, particularly the epidermis, where it pairs with cytokeratin 1 (CK1) to maintain skin integrity and regulate terminal differentiation of keratinocytes. Its expression is tightly linked to epidermal development and barrier formation.
Recombinant CK10 is produced using biotechnological systems (e.g., E. coli, mammalian cells) to ensure high purity and biological activity. The protein is engineered with tags (e.g., His-tag) for simplified purification and detection. Research applications include studying skin biology, investigating hyperproliferative disorders (e.g., psoriasis, ichthyosis), and exploring genetic mutations causing skin fragility syndromes like epidermolytic hyperkeratosis. CK10 dysregulation is also implicated in cancer progression, particularly in epithelial-derived malignancies.
In drug development, recombinant CK10 serves as an antigen for antibody production or a substrate for testing therapeutic agents targeting keratin-related pathologies. Its role in 3D skin models and organotypic cultures further underscores its utility in dermatological research. Despite functional redundancy among keratins, CK10 remains a critical biomarker for studying epidermal homeostasis and disease mechanisms. Ongoing studies aim to elucidate its non-mechanical roles, including signaling crosstalk and stress responses in skin cells.
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