| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CK8 |
| Uniprot No | P05787 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-483aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMSIRVTQKSYKVSTSGPRAFSSRSYTS GPGSRISSSSFSRVGSSNFRGGLGGGYGGASGMGGITAVTVNQSLLSPLV LEVDPNIQAVRTQEKEQIKTLNNKFASFIDKVRFLEQQNKMLETKWSLLQ QQKTARSNMDNMFESYINNLRRQLETLGQEKLKLEAELGNMQGLVEDFKN KYEDEINKRTEMENEFVLIKKDVDEAYMNKVELESRLEGLTDEINFLRQL YEEEIRELQSQISDTSVVLSMDNSRSLDMDSIIAEVKAQYEDIANRSRAE AESMYQIKYEELQSLAGKHGDDLRRTKTEISEMNRNISRLQAEIEGLKGQ RASLEAAIADAEQRGELAIKDANAKLSELEAALQRAKQDMARQLREYQEL MNVKLALDIEIATYRKLLEGEESRLESGMQNMSIHTKTTSGYAGGLSSAY GGLTSPGLSYSLGSSFGSGAGSSSFSRTSSSRAVVVKKIETRDGKLVSES SDVLPK |
| 预测分子量 | 56 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CK8重组蛋白的模拟参考文献示例(仅供参考，具体文献需通过学术数据库核实)：
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1. **文献名称**：*Expression and Purification of Recombinant Human Cytokeratin 8 in Escherichia coli for Antibody Production*
**作者**：Zhang Y, et al.
**摘要**：本研究利用大肠杆菌系统成功表达并纯化了重组人CK8蛋白，优化了诱导条件以提高溶解度。纯化后的蛋白用于制备单克隆抗体，验证了其在免疫组化中的应用，为上皮源性肿瘤诊断提供工具。
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2. **文献名称**：*Structural Characterization of Recombinant Cytokeratin 8 and Its Role in Intermediate Filament Assembly*
**作者**：Lee S, et al.
**摘要**：通过哺乳动物表达系统获得重组CK8蛋白，结合X射线晶体学分析其结构，揭示了CK8单体通过螺旋域相互作用形成二聚体的机制，为中间丝动态组装提供分子依据。
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3. **文献名称**：*Recombinant CK8 as a Biomarker for Drug-Induced Liver Injury in Vitro Models*
**作者**：García-Sánchez A, et al.
**摘要**：构建重组CK8蛋白并用于体外肝细胞损伤模型，证实其释放水平与药物毒性呈正相关，提出CK8可作为肝毒性的早期检测标志物。
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4. **文献名称**：*CK8 Recombinant Protein-Based ELISA for Monitoring Metastatic Carcinoma Progression*
**作者**：Chen L, et al.
**摘要**：开发基于重组CK8的ELISA检测法，分析患者血清CK8水平与肿瘤转移的相关性，结果显示高灵敏度，支持其在癌症预后评估中的潜在价值。
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建议通过PubMed、Google Scholar等平台以关键词“recombinant cytokeratin 8”或“CK8 protein expression”检索最新文献获取准确信息。
Cytokeratin 8 (CK8), a member of the intermediate filament protein family, is a key structural component of epithelial cells. It is predominantly expressed in single-layered epithelial tissues, such as those lining the liver, intestine, and glandular organs. CK8 pairs with its partner cytokeratin 18 (CK18) to form heteropolymers, which contribute to cellular integrity, mechanical stress resistance, and organelle organization. These keratins play critical roles in maintaining cell shape, facilitating cell signaling, and protecting against apoptosis. Dysregulation of CK8 expression or mutations in its gene (KRT8) have been linked to liver diseases, inflammatory bowel disorders, and epithelial cancers, making it a biomarker of interest in pathological studies.
Recombinant CK8 protein is engineered using biotechnological platforms, such as bacterial (E. coli) or mammalian expression systems, to produce purified, functional CK8 for research and diagnostic applications. This in vitro-expressed protein retains epitopes and biochemical properties similar to native CK8. enabling its use in antibody validation, protein interaction studies, and disease modeling. Researchers employ recombinant CK8 to investigate its role in tumor progression, epithelial-mesenchymal transition (EMT), and drug resistance mechanisms. In diagnostics, it serves as an antigen for detecting CK8-specific autoantibodies in autoimmune diseases or monitoring tissue damage in conditions like hepatitis. Additionally, recombinant CK8 is utilized in developing cell culture models to study epithelial barrier function and cytotoxicity. Its standardized production ensures reproducibility in studies aiming to unravel CK8-related cellular pathways or therapeutic targets.
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