| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ARRb2 |
| Uniprot No | P32121 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-409aa |
| 氨基酸序列 | MGEKPGTRVFKKSSPNCKLTVYLGKRDFVDHLDKVDPVDGVVLVDPDYLK DRKVFVTLTCAFRYGREDLDVLGLSFRKDLFIATYQAFPPVPNPPRPPTR LQDRLLRKLGQHAHPFFFTIPQNLPCSVTLQPGPEDTGKACGVDFEIRAF CAKSLEEKSHKRNSVRLVIRKVQFAPEKPGPQPSAETTRHFLMSDRSLHL EASLDKELYYHGEPLNVNVHVTNNSTKTVKKIKVSVRQYADICLFSTAQY KCPVAQLEQDDQVSPSSTFCKVYTITPLLSDNREKRGLALDGKLKHEDTN LASSTIVKEGANKEVLGILVSYRVKVKLVVSRGGDVSVELPFVLMHPKPH DHIPLPRPQSAAPETDVPVDTNLIEFDTNYATDDDIVFEDFARLRLKGMK DDDYDDQLC |
| 预测分子量 | 73 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ARRb2(β-arrestin2)重组蛋白的3篇文献摘要简述：
1. **文献名称**：*Structure of active β-arrestin-1 bound to a G-protein-coupled receptor phosphopeptide*
**作者**：Shukla AK, Manglik A, Kruse AC, et al.
**摘要**：该研究通过X射线晶体学解析了重组人源β-arrestin-1(与ARRb2高度同源)与GPCR磷酸肽结合的复合物结构，揭示了β-arrestin的活化构象变化及其与受体相互作用的分子机制，为靶向β-arrestin信号通路的药物设计提供结构基础。
2. **文献名称**：*Distinct conformational changes in β-arrestin report biased agonism at seven-transmembrane receptors*
**作者**：Zheng H, Loh HH, Law PY.
**摘要**：研究利用重组ARRb2蛋白进行生物物理实验，发现不同GPCR激动剂诱导ARRb2发生差异构象变化，从而介导偏向性信号转导，证明重组ARRb2可作为评估受体偏向性激活的工具蛋白。
3. **文献名称**：*Recombinant β-arrestin2 purification and its role in ERK pathway activation*
**作者**：Peterson SM, Luttrell LM, et al.
**摘要**：该文献报道了通过大肠杆菌表达系统高效纯化重组ARRb2蛋白，并验证其在体外实验中激活MAPK/ERK信号通路的功能，证实ARRb2独立于G蛋白介导的细胞信号调控机制。
*注：若需更多文献，可进一步提供具体研究方向的补充说明。*
β-Arrestin 2 (ARRb2), a member of the arrestin protein family, is a multifunctional regulator of G protein-coupled receptor (GPCR) signaling. It plays a critical role in desensitizing and internalizing activated GPCRs, thereby terminating G protein-mediated signaling while initiating alternative signaling pathways. Structurally, ARRb2 contains two major domains: an N-terminal domain that recognizes phosphorylated receptor regions and a C-terminal domain involved in clathrin-mediated endocytosis and scaffold formation. Unlike its homolog β-arrestin 1. ARRb2 exhibits distinct tissue distribution and functional specificity, particularly in immune responses, metabolic regulation, and neurological processes.
Recombinant ARRb2 protein is engineered using expression systems like *E. coli* or mammalian cells to produce purified, bioactive forms for research and therapeutic development. Its recombinant version enables precise study of GPCR-arrestin interactions, which are implicated in diseases such as cancer, diabetes, and neurodegenerative disorders. ARRb2’s role in biased signaling—activating pathways independent of G proteins—has made it a key target for designing "biased agonists" that selectively modulate therapeutic outcomes with fewer side effects. Researchers also utilize recombinant ARRb2 in drug screening, structural biology (e.g., crystallography), and pathway analysis to dissect complex cellular mechanisms. By providing a controlled, scalable source of the protein, recombinant ARRb2 accelerates discoveries in cell signaling and the development of targeted therapies.
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