| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CK13 |
| Uniprot No | P13646 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-458aa |
| 氨基酸序列 | MSLRLQSSSA SYGGGFGGGS CQLGGGRGVS TCSTRFVSGG SAGGYGGGVS CGFGGGAGSG FGGGYGGGLG GGYGGGLGGG FGGGFAGGFV DFGACDGGLL TGNEKITMQN LNDRLASYLE KVRALEEANA DLEVKIRDWH LKQSPASPER DYSPYYKTIE ELRDKILTAT IENNRVILEI DNARLAADDF RLKYENELAL RQSVEADING LRRVLDELTL SKTDLEMQIE SLNEELAYMK KNHEEEMKEF SNQVVGQVNV EMDATPGIDL TRVLAEMREQ YEAMAERNRR DAEEWFHTKS AELNKEVSTN TAMIQTSKTE ITELRRTLQG LEIELQSQLS MKAGLENTVA ETECRYALQL QQIQGLISSI EAQLSELRSE MECQNQEYKM LLDIKTRLEQ EIATYRSLLE GQDAKMIGFP SSAGSVSPRS TSVTTTSSAS VTTTSNASGR RTSDVRRP |
| 预测分子量 | 49,5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CK13重组蛋白的3篇参考文献概览：
1. **文献名称**：*"Expression and purification of recombinant human cytokeratin 13 in Escherichia coli for antibody production"*
**作者**：Smith A, et al.
**摘要**：该研究利用大肠杆菌系统成功表达了重组人CK13蛋白，并优化了纯化条件。通过Western blot验证其抗原性，为制备高特异性CK13抗体奠定了基础，适用于口腔上皮病变的免疫检测。
2. **文献名称**：*"Structural characterization of recombinant cytokeratin 13 and its interaction with oral squamous cell carcinoma biomarkers"*
**作者**：Lee J, et al.
**摘要**：通过哺乳动物细胞表达体系获得可溶性CK13重组蛋白，分析其二级结构及与口腔鳞癌标志物(如MMP-9)的相互作用，揭示CK13在肿瘤微环境中的潜在调控机制。
3. **文献名称**：*"Development of a CK13-based ELISA assay for early diagnosis of esophageal dysplasia"*
**作者**：Zhang Y, et al.
**摘要**：研究构建了基于重组CK13蛋白的ELISA检测方法，发现食管上皮异型增生患者血清中CK13抗体水平显著升高，提示其作为无创诊断工具的临床价值。
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**注**：以上文献信息为示例性概括，实际文献需通过学术数据库检索确认。若需具体文章，建议使用关键词(如“recombinant CK13 protein”、“cytokeratin 13 expression”)在PubMed或Web of Science中查找。
CK13 (Cytokeratin 13), a member of the type I keratin family, is an intermediate filament protein predominantly expressed in stratified epithelial tissues, including mucosal surfaces of the oral cavity, esophagus, and cervix. As part of the cytokeratin family, it plays a structural role in maintaining cellular integrity and resilience against mechanical stress. Recombinant CK13 protein is engineered through genetic engineering techniques, typically expressed in bacterial (e.g., *E. coli*) or mammalian cell systems to ensure proper folding and post-translational modifications. Its molecular weight ranges between 51-54 kDa, featuring characteristic α-helical rod domains flanked by non-helical N- and C-terminal regions.
Research on recombinant CK13 focuses on its biological functions and pathological associations. It serves as a differentiation marker for non-cornified squamous epithelia and is downregulated in epithelial-mesenchymal transition (EMT) during cancer progression. In diagnostics, CK13 expression loss correlates with oral squamous cell carcinoma and premalignant lesions, making it a potential biomarker. Therapeutically, recombinant CK13 aids in studying epithelial barrier dysfunction, wound healing, and tissue regeneration models. It is also utilized in antibody production for immunohistochemistry and ELISA-based assays.
Recent studies explore CK13's role in cellular signaling, particularly its interaction with desmosomal proteins and inflammatory mediators. Challenges in recombinant production include preserving native conformational epitopes and phosphorylation sites critical for functional studies. Advances in protein engineering, such as codon optimization and mammalian expression systems, have improved its utility in translational research targeting epithelial disorders and personalized oncology.
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