| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NFkB2 |
| Uniprot No | Q00653 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-900aa |
| 氨基酸序列 | MESCYNPGLDGIIEYDDFKLNSSIVEPKEPAPETADGPYLVIVEQPKQRGFRFRYGCEGPSHGGLPGASSEKGRKTYPTVKICNYEGPAKIEVDLVTHSDPPRAHAHSLVGKQCSELGICAVSVGPKDMTAQFNNLGVLHVTKKNMMGTMIQKLQRQRLRSRPQGLTEAEQRELEQEAKELKKVMDLSIVRLRFSAFLRASDGSFSLPLKPVISQPIHDSKSPGASNLKISRMDKTAGSVRGGDEVYLLCDKVQKDDIEVRFYEDDENGWQAFGDFSPTDVHKQYAIVFRTPPYHKMKIERPVTVFLQLKRKRGGDVSDSKQFTYYPLVEDKEEVQRKRRKALPTFSQPFGGGSHMGGGSGGAAGGYGGAGGGGSLGFFPSSLAYSPYQSGAGPMGCYPGGGGGAQMAATVPSRDSGEEAAEPSAPSRTPQCEPQAPEMLQRAREYNARLFGLAQRSARALLDYGVTADARALLAGQRHLLTAQDENGDTPLHLAIIHGQTSVIEQIVYVIHHAQDLGVVNLTNHLHQTPLHLAVITGQTSVVSFLLRVGADPALLDRHGDSAMHLALRAGAGAPELLRALLQSGAPAVPQLLHMPDFEGLYPVHLAVRARSPECLDLLVDSGAEVEATERQGGRTALHLATEMEELGLVTHLVTKLRANVNARTFAGNTPLHLAAGLGYPTLTRLLLKAGADIHAENEEPLCPLPSPPTSDSDSDSEGPEKDTRSSFRGHTPLDLTCSTKVKTLLLNAAQNTMEPPLTPPSPAGPGLSLGDTALQNLEQLLDGPEAQGSWAELAERLGLRSLVDTYRQTTSPSGSLLRSYELAGGDLAGLLEALSDMGLEEGVRLLRGPETRDKLPSTAEVKEDSAYGSQSVEQEAEKLGPPPEPPGGLCHGHPQPQVH |
| 预测分子量 | 96,7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NFκB2重组蛋白的3篇参考文献概览：
1. **《Recombinant NF-κB2/p52 protein production and functional analysis in DNA binding》**
- 作者：Chen et al.
- 摘要：研究报道了通过大肠杆菌系统表达并纯化NFκB2/p52重组蛋白，验证其与DNA的结合活性，揭示了p52在调控靶基因中的结构基础。
2. **《Structural insights into NF-κB p52/p52 homodimer assembly using recombinant protein》**
- 作者：Wang et al.
- 摘要：利用昆虫细胞表达系统制备NFκB2/p52重组蛋白，通过X射线晶体学解析其同源二聚体结构，阐明了其与IκB抑制蛋白的相互作用机制。
3. **《NF-κB2/p100 processing to p52 in a cell-free system: Role of recombinant proteasomal subunits》**
- 作者：Smith et al.
- 摘要：开发了基于重组蛋白酶体的体外实验体系，证明p100向p52的加工依赖蛋白酶体活性，为NFκB2信号通路调控提供了生化证据。
*注：上述文献为示例，实际引用时请核实具体文章信息。建议通过PubMed或Google Scholar以“NFκB2 recombinant protein”为关键词检索最新研究。*
Nuclear factor kappa B subunit 2 (NFκB2), a member of the NF-κB transcription factor family, plays critical roles in immune regulation, inflammation, and cell survival. The NF-κB family comprises five subunits (RelA/p65. RelB, c-Rel, NFκB1/p50. and NFκB2/p52) that form homo- or heterodimers to regulate gene expression. NFκB2 is synthesized as a precursor protein, p100. which contains an N-terminal Rel homology domain and a C-terminal inhibitory domain. In the non-canonical NF-κB signaling pathway, stimuli such as lymphotoxin-β receptor (LTβR) or B-cell activating factor (BAFF) activate NFκB-inducing kinase (NIK), leading to phosphorylation and proteasomal processing of p100 into the mature p52 subunit. This active form translocates to the nucleus, partnering with RelB to regulate genes involved in lymphoid organ development, B-cell maturation, and adaptive immunity.
Recombinant NFκB2 proteins, produced via bacterial, mammalian, or insect expression systems, are essential tools for studying NF-κB signaling mechanisms. These proteins retain functional domains for DNA binding, dimerization, and interaction with inhibitors like IκB. Researchers utilize recombinant NFκB2 in assays such as electrophoretic mobility shift assays (EMSAs), chromatin immunoprecipitation (ChIP), and co-immunoprecipitation to investigate its binding partners, post-translational modifications, and regulatory pathways. Dysregulation of NFκB2 is implicated in cancers, autoimmune disorders, and chronic inflammation, making it a therapeutic target. Recombinant forms enable drug screening and structural studies to design inhibitors that block aberrant NF-κB activation. Additionally, engineered mutants (e.g., phosphorylation-deficient variants) help dissect signaling cascades. Despite its overlapping functions with NFκB1/p50. NFκB2/p52 exhibits unique roles in immune homeostasis, emphasizing the need for isoform-specific research tools. Ongoing studies focus on its context-dependent interactions and therapeutic modulation in diseases like multiple myeloma and rheumatoid arthritis.
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