| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CD10 |
| Uniprot No | P08473 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 53-750aa |
| 氨基酸序列 | DDGICKSSDCIKSAARLIQNMDATTEPCTDFFKYACGGWLKRNVIPETSS RYGNFDILRDELEVVLKDVLQEPKTEDIVAVQKAKALYRSCINESAIDSR GGEPLLKLLPDIYGWPVATENWEQKYGASWTAEKAIAQLNSKYGKKVLIN LFVGTDDKNSVNHVIHIDQPRLGLPSRDYYECTGIYKEACTAYVDFMISV ARLIRQEERLPIDENQLALEMNKVMELEKEIANATAKPEDRNDPMLLYNK MTLAQIQNNFSLEINGKPFSWLNFTNEIMSTVNISITNEEDVVVYAPEYL TKLKPILTKYSARDLQNLMSWRFIMDLVSSLSRTYKESRNAFRKALYGTT SETATWRRCANYVNGNMENAVGRLYVEAAFAGESKHVVEDLIAQIREVFI QTLDDLTWMDAETKKRAEEKALAIKERIGYPDDIVSNDNKLNNEYLELNY KEDEYFENIIQNLKFSQSKQLKKLREKVDKDEWISGAAVVNAFYSSGRNQ IVFPAGILQPPFFSAQQSNSLNYGGIGMVIGHEITHGFDDNGRNFNKDGD LVDWWTQQSASNFKEQSQCMVYQYGNFSWDLAGGQHLNGINTLGENIADN GGLGQAYRAYQNYIKKNGEEKLLPGLDLNHKQLFFLNFAQVWCGTYRPEY AVNSIKTDVHSPGNFRIIGTLQNSAEFSEAFHCRKNSYMNPEKKCRVW |
| 预测分子量 | 85 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CD10重组蛋白的3-4篇虚构参考文献示例，供参考：
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1. **文献名称**：*Recombinant CD10 Expression in E. coli: Purification and Enzymatic Characterization*
**作者**：Zhang Y et al.
**摘要**：本研究通过大肠杆菌系统成功表达并纯化了重组CD10蛋白，优化了诱导条件以提高可溶性表达。纯化后的蛋白显示与天然CD10相似的酶活性，能够有效水解底物脑啡肽，为后续功能研究提供了工具。
2. **文献名称**：*Structural Insights into CD10/NEP by Cryo-EM: Implications for Substrate Recognition*
**作者**：Johnson R et al.
**摘要**：利用冷冻电镜技术解析了重组CD10的3D结构，揭示了其锌离子结合域和底物结合口袋的关键氨基酸残基，阐明了其选择性切割神经肽的分子机制，为设计靶向抑制剂奠定基础。
3. **文献名称**：*CD10 as a Biomarker in Triple-Negative Breast Cancer: Role of Recombinant Protein in Functional Assays*
**作者**：Lee S et al.
**摘要**：通过重组CD10蛋白验证了其在三阴性乳腺癌细胞中的高表达，并证明其通过降解趋化因子CXCL12促进肿瘤转移，提示CD10可能成为治疗靶点。
4. **文献名称**：*Recombinant CD10 Attenuates Inflammatory Response in Macrophages via Neuropeptide Degradation*
**作者**：Garcia M et al.
**摘要**：研究发现外源性重组CD10可通过降解促炎神经肽(如P物质)，显著抑制巨噬细胞中NF-κB通路活化，减轻炎症反应，提示其在自身免疫疾病中的潜在治疗价值。
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注：以上文献为示例，实际引用时需以真实发表的论文为准。建议通过PubMed、Web of Science等平台检索关键词“recombinant CD10”“NEP protein”等获取文献。
**Background of CD10 Recombinant Protein**
CD10. also known as neutral endopeptidase (NEP) or membrane metallo-endopeptidase (MME), is a cell surface zinc-dependent metalloprotease encoded by the *MME* gene. It plays a critical role in regulating bioactive peptides by cleaving and inactivating various substrates, including peptide hormones (e.g., enkephalins, endothelin), cytokines, and neuropeptides. CD10 is broadly expressed in tissues such as kidney, intestine, and immune cells, and serves as a biomarker for certain cancers, including acute lymphoblastic leukemia and stromal tumors.
Structurally, CD10 is a type II transmembrane glycoprotein (~90–110 kDa) with a short N-terminal cytoplasmic domain, a transmembrane region, and a large extracellular C-terminal catalytic domain. Its enzymatic activity depends on zinc ions and a conserved HEXXH motif. Dysregulation of CD10/NEP has been linked to pathologies such as Alzheimer’s disease, hypertension, and cancer progression, where it modulates cell signaling, adhesion, and apoptosis.
Recombinant CD10 protein is engineered using mammalian expression systems (e.g., HEK293 or CHO cells) to ensure proper post-translational modifications and functional activity. It is produced as a soluble or membrane-bound form, often fused with tags (e.g., Fc, His) for purification and detection. This recombinant tool enables *in vitro* studies of CD10’s enzymatic mechanisms, substrate specificity, and interactions with inhibitors. It is also used in drug discovery (e.g., developing NEP inhibitors like sacubitril for heart failure) and diagnostic assays.
By providing a controlled source of active CD10. recombinant protein aids in deciphering its roles in health and disease, offering potential therapeutic and biomarker applications.
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