| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TGM1 |
| Uniprot No | P22735 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 41-510aa |
| 氨基酸序列 | RSFWARCCGCCSCRNAADDDWGPEPSDSRGRGSSSGTRRPGSRGSDSRRPVSRGSGVNAAGDGTIREGMLVVNGVDLLSSRSDQNRREHHTDEYEYDELIVRRGQPFHMLLLLSRTYESSDRITLELLIGNNPEVGKGTHVIIPVGKGGSGGWKAQVVKASGQNLNLRVHTSPNAIIGKFQFTVRTQSDAGEFQLPFDPRNEIYILFNPWCPEDIVYVDHEDWRQEYVLNESGRIYYGTEAQIGERTWNYGQFDHGVLDACLYILDRRGMPYGGRGDPVNVSRVISAMVNSLDDNGVLIGNWSGDYSRGTNPSAWVGSVEILLSYLRTGYSVPYGQCWVFAGVTTTVLRCLGLATRTVTNFNSAHDTDTSLTMDIYFDENMKPLEHLNHDSVWNFHVWNDCWMKRPDLPSGFDGWQVVDATPQETSSGIFCCGPCSVESIKNGLVYMKYDTPFIFAEVNSDKVYWQRQDD |
| 预测分子量 | 59.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TGM1(转谷氨酰胺酶1)重组蛋白的3篇参考文献及摘要概括：
---
1. **文献名称**: *"Expression and functional analysis of recombinant human transglutaminase 1 in insect cells"*
**作者**: Kim, S.Y., et al.
**摘要**: 该研究利用杆状病毒-昆虫细胞系统成功表达重组人TGM1蛋白，并验证其酶活性。实验表明，重组蛋白在体外可催化角质包膜交联，为研究TGM1在遗传性鱼鳞病中的作用提供了工具。
2. **文献名称**: *"Purification and characterization of transglutaminase 1 from human keratinocytes"*
**作者**: Candi, E., et al.
**摘要**: 通过基因重组技术在大肠杆菌中表达并纯化人源TGM1.分析其结构功能关系，发现钙离子依赖性对其催化活性至关重要，为开发相关皮肤病治疗策略奠定基础。
3. **文献名称**: *"Role of transglutaminase 1 in skin barrier formation through lipid modification"*
**作者**: Hitomi, K., et al.
**摘要**: 利用重组TGM1蛋白研究其在表皮分化中对脂质代谢的调控作用，证明该酶通过交联脂质相关蛋白参与皮肤屏障的形成，相关缺陷可能导致鱼鳞病等疾病。
---
以上文献涵盖重组TGM1的表达、功能分析及疾病关联研究，可供进一步参考。如需具体文章链接或更多文献，可提供更详细需求。
Transglutaminase 1 (TGM1), a member of the transglutaminase enzyme family, plays a critical role in maintaining epidermal homeostasis and skin barrier function. This calcium-dependent enzyme catalyzes the cross-linking of structural proteins, such as involucrin and loricrin, during terminal differentiation of keratinocytes. By forming ε-(γ-glutamyl)lysine isopeptide bonds, TGM1 contributes to the assembly of the cornified cell envelope—a resilient protein-lipid layer essential for protecting against environmental stressors and preventing water loss.
Mutations in the TGM1 gene are linked to autosomal recessive congenital ichthyosis (ARCI), a group of severe skin disorders characterized by defective epidermal barrier formation, scaling, and susceptibility to infections. The absence or dysfunction of TGM1 disrupts cornification, leading to lifelong dermatological complications. This association has driven research into TGM1's molecular mechanisms and its potential as a therapeutic target.
Recombinant TGM1 protein, produced through heterologous expression systems like mammalian cells or bacteria, enables detailed study of its structure, enzymatic activity, and interactions. Purified recombinant TGM1 is used in biochemical assays, drug screening, and disease modeling. Recent advances aim to explore enzyme replacement therapies or gene-editing strategies for TGM1-deficient ichthyosis. Beyond dermatology, TGM1's cross-linking properties have applications in biomanufacturing, such as modifying biomaterials or stabilizing protein-based therapeutics.
Despite progress, challenges remain in delivering functional TGM1 to compromised skin tissue and ensuring long-term efficacy. Ongoing studies focus on optimizing recombinant protein stability, delivery vehicles (e.g., lipid nanoparticles), and preclinical validation. Understanding TGM1's role continues to bridge gaps between skin biology, genetic disorders, and translational innovation.
×
