| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CK17 |
| Uniprot No | Q04695 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-432aa |
| 氨基酸序列 | MTTSIRQFTSSSSIKGSSGLGGGSSRTSCRLSGGLGAGSCRLGSAGGLGS TLGGSSYSSCYSFGSGGGYGSSFGGVDGLLAGGEKATMQNLNDRLASYLD KVRALEEANTELEVKIRDWYQRQAPGPARDYSQYYRTIEELQNKILTATV DNANILLQIDNARLAADDFRTKFETEQALRLSVEADINGLRRVLDELTLA RADLEMQIENLKEELAYLKKNHEEEMNALRGQVGGEINVEMDAAPGVDLS RIPNEMRDQYEKMAEKNRKDAEDWFFSKTEELNREVATNSELVQSGKSEI SELRRTMQALEIELQSQLSMKASLEGNLAETENRYCVQLSQIQGLIGSVE EQLAQLRCEMEQQNQEYKILLDVKTRLEQEIATYRRLLEGEDAHLTQYKK EPVTTRQVRTIVEEVQDGKVISSREQVHQTTR |
| 预测分子量 | 73 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CK17重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**：*"Recombinant Keratin 17 Expression in Human Tumors: Association with Malignant Progression"*
**作者**：Smith A, et al.
**摘要**：该研究通过重组表达CK17蛋白，分析其在多种肿瘤组织中的分布，发现CK17高表达与肿瘤侵袭性及不良预后相关，提示其可作为癌症进展的生物标志物。
2. **文献名称**：*"Functional Characterization of Recombinant Keratin 17 in Epithelial Cell Migration"*
**作者**：Chen L, et al.
**摘要**：利用重组CK17蛋白研究其在细胞迁移中的作用，发现CK17通过调节细胞骨架重组和整合素信号通路，促进上皮细胞迁移，可能参与伤口愈合和肿瘤转移。
3. **文献名称**：*"Production and Immunogenicity Evaluation of Recombinant CK17 for Serodiagnosis in Cancer"*
**作者**：Wang Y, et al.
**摘要**：该研究开发了一种重组CK17蛋白的高效表达及纯化方法，并验证其在血清学检测中的应用，显示其在卵巢癌和宫颈癌患者中具有高特异性抗体反应。
(注：以上文献信息为示例，实际文献需通过数据库如PubMed检索确认。)
CK17. or cytokeratin 17. is a member of the intermediate filament protein family that plays critical roles in maintaining structural integrity and cellular stress responses in epithelial tissues. It is primarily expressed in basal epithelial cells, hair follicles, and certain glandular tissues under normal physiological conditions. However, CK17 has gained significant attention due to its aberrant overexpression in various pathological states, particularly cancers. Studies have linked elevated CK17 levels to aggressive tumor behavior, including squamous cell carcinomas, breast cancer, and pancreatic ductal adenocarcinoma, where it serves as a biomarker for poor prognosis and therapeutic resistance.
Recombinant CK17 protein is engineered through molecular cloning techniques, typically expressed in prokaryotic (e.g., E. coli) or eukaryotic systems (e.g., mammalian cells) to ensure proper post-translational modifications. This bioengineered protein retains the functional domains of native CK17. enabling researchers to study its molecular interactions, signaling pathways, and regulatory mechanisms in controlled experimental settings. Its applications span basic research, diagnostic development, and therapeutic exploration. In cancer biology, recombinant CK17 helps investigate its role in promoting cell proliferation, migration, and epithelial-mesenchymal transition (EMT). Additionally, it aids in understanding CK17's involvement in inflammatory skin disorders like psoriasis and chronic wound healing.
The production of recombinant CK17 also supports antibody generation for immunohistochemical diagnostics, enhancing precision in cancer subtyping and stratification. Furthermore, it serves as a potential target for drug discovery aimed at modulating CK17-mediated pathways. As research progresses, CK17 continues to emerge as a multifunctional protein bridging structural stability and cellular signaling, making its recombinant form a valuable tool for both academic and clinical investigations.
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