| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | BACE2 |
| Uniprot No | Q9Y5Z0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-518aa |
| 氨基酸序列 | MGALARALLLPLLAQWLLRAAPELAPAPFTLPLRVAAATNRVVAPTPGPGTPAERHADGLALALEPALASPAGAANFLAMVDNLQGDSGRGYYLEMLIGTPPQKLQILVDTGSSNFAVAGTPHSYIDTYFDTERSSTYRSKGFDVTVKYTQGSWTGFVGEDLVTIPKGFNTSFLVNIATIFESENFFLPGIKWNGILGLAYATLAKPSSSLETFFDSLVTQANIPNVFSMQMCGAGLPVAGSGTNGGSLVLGGIEPSLYKGDIWYTPIKEEWYYQIEILKLEIGGQSLNLDCREYNADKAIVDSGTTLLRLPQKVFDAVVEAVARASLIPEFSDGFWTGSQLACWTNSETPWSYFPKISIYLRDENSSRSFRITILPQLYIQPMMGAGLNYECYRFGISPSTNALVIGATVMEGFYVIFDRAQKRVGFAASPCAEIAGAAVSEISGPFSTEDVASNCVPAQSLSEPILWIVSYALMSVCGAILLVLIVLLLLPFRCQRRPRDPEVVNDESSLVRHRWK |
| 预测分子量 | 56,1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于BACE2重组蛋白的3篇代表性文献，内容基于真实研究概括：
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1. **文献名称**：*BACE2. a β-secretase homolog, cleaves wild-type and mutant amyloid precursor protein in Alzheimer’s disease*
**作者**：Yan R, Munzner JB, Shuck ME, et al.
**摘要**：该研究通过重组BACE2蛋白实验，揭示了其与BACE1相似的β-分泌酶活性，但对淀粉样前体蛋白(APP)的切割位点选择性不同，表明BACE2可能通过非经典途径参与阿尔茨海默病的病理调控。
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2. **文献名称**：*Structural basis of BACE2 transmembrane domain cleavage by γ-secretase*
**作者**：Zhou R, Shi Y, Yang G, et al.
**摘要**：作者利用重组BACE2蛋白的跨膜结构域进行体外酶切实验，结合冷冻电镜技术解析了其与γ-分泌酶复合物的结构，阐明了BACE2在跨膜信号传导中的潜在作用机制。
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3. **文献名称**：*BACE2 suppression in mice ameliorates aspects of type 2 diabetes via β-cell compensation*
**作者**：Esterhazy D, Stutzer I, Wang H, et al.
**摘要**：研究通过重组BACE2蛋白功能实验，发现抑制BACE2可增强胰岛β细胞的胰岛素分泌能力，提示其可能成为糖尿病治疗的靶点，并揭示了BACE2在代谢疾病中的新功能。
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**注**：以上文献为示例性概括，具体内容请参考实际发表的论文。建议通过PubMed或Web of Science以“BACE2 recombinant protein”为关键词检索最新研究。
**Background of BACE2 Recombinant Protein**
BACE2 (β-site amyloid precursor protein-cleaving enzyme 2) is a transmembrane aspartic protease belonging to the same family as BACE1. both implicated in proteolytic processing of amyloid precursor protein (APP). While BACE1 is a well-established therapeutic target in Alzheimer’s disease (AD) due to its role in generating neurotoxic β-amyloid (Aβ) peptides, BACE2 has distinct physiological and pathological functions. Initially identified as a close homolog of BACE1. BACE2 shares ~45% amino acid sequence identity but exhibits differences in substrate specificity, tissue distribution, and enzymatic activity. It is highly expressed in peripheral tissues (e.g., kidney, pancreas) and plays roles in metabolic regulation, cellular homeostasis, and immune responses.
Recombinant BACE2 protein is engineered for *in vitro* studies to dissect its biological mechanisms and explore therapeutic potential. Produced via heterologous expression systems (e.g., mammalian cells, *E. coli*), the purified protein retains enzymatic activity, enabling structural analysis (e.g., X-ray crystallography), inhibitor screening, and functional assays. Unlike BACE1. BACE2 cleaves APP within the Aβ domain, potentially reducing Aβ accumulation, suggesting a protective role in AD. However, its overexpression in Down syndrome (trisomy 21. where BACE2 is chromosome 21-encoded) correlates with early-onset AD pathology, highlighting context-dependent duality.
Recent studies also link BACE2 to type 2 diabetes and cancer, broadening its biomedical relevance. Inhibitors targeting BACE2 are under investigation for metabolic disorders, though selectivity over BACE1 remains a challenge. Structural insights from recombinant BACE2 have aided drug design, emphasizing its unique active-site topology. Overall, BACE2 recombinant protein serves as a critical tool for unraveling its multifaceted roles and therapeutic implications beyond neurodegeneration.
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