| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PXN |
| Uniprot No | P49023 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-591aa |
| 氨基酸序列 | MDDLDALLAD LESTTSHISK RPVFLSEETP YSYPTGNHTY QEIAVPPPVP PPPSSEALNG TILDPLDQWQ PSSSRFIHQQ PQSSSPVYGS SAKTSSVSNP QDSVGSPCSR VGEEEHVYSF PNKQKSAEPS PTVMSTSLGS NLSELDRLLL ELNAVQHNPP GFPADEANSS PPLPGALSPL YGVPETNSPL GGKAGPLTKE KPKRNGGRGL EDVRPSVESL LDELESSVPS PVPAITVNQG EMSSPQRVTS TQQQTRISAS SATRELDELM ASLSDFKIQG LEQRADGERC WAAGWPRDGG RSSPGGQDEG GFMAQGKTGS SSPPGGPPKP GSQLDSMLGS LQSDLNKLGV ATVAKGVCGA CKKPIAGQVV TAMGKTWHPE HFVCTHCQEE IGSRNFFERD GQPYCEKDYH NLFSPRCYYC NGPILDKVVT ALDRTWHPEH FFCAQCGAFF GPEGFHEKDG KAYCRKDYFD MFAPKCGGCA RAILENYISA LNTLWHPECF VCRECFTPFV NGSFFEHDGQ PYCEVHYHER RGSLCSGCQK PITGRCITAM AKKFHPEHFV CAFCLKQLNK GTFKEQNDKP YCQNCFLKLF C |
| 预测分子量 | 64,5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3篇关于PXN(Paxillin)重组蛋白的代表性文献摘要信息：
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1. **文献名称**: *"Recombinant Paxillin Phosphorylation Biomarkers in Cancer Cell Migration Studies"*
**作者**: Chen, L. et al.
**摘要**: 研究利用重组PXN蛋白分析其酪氨酸磷酸化位点(Y31/Y118)在肿瘤细胞迁移中的作用，发现磷酸化修饰通过调节FAK信号通路影响细胞黏附动力学。
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2. **文献名称**: *"Expression and Purification of Functional Paxillin LD4 Motif for Integrin Binding Assays"*
**作者**: Turner, C.E. & Miller, J.R.
**摘要**: 报道了通过大肠杆菌表达系统高效制备重组PXN蛋白的LD4结构域，验证其与整合素β亚基的相互作用，为细胞外基质信号传导机制提供实验工具。
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3. **文献名称**: *"Structural Insights into Paxillin-LIMK Complex by Cryo-EM"*
**作者**: Wang, Y. et al.
**摘要**: 通过冷冻电镜解析重组PXN蛋白与LIM激酶(LIMK)的复合物结构，阐明PXN在细胞骨架重塑中的构象变化及调控机制。
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**备注**：以上文献信息为模拟示例，实际研究需通过PubMed/Google Scholar等平台检索关键词(如"Paxillin recombinant protein" "PXN signaling")获取具体文献。建议优先选择近五年内发表于《JBC》《Cell Communication and Signaling》等期刊的高引论文。
Paxillin (PXN) is a multifunctional adaptor protein primarily involved in cell adhesion, migration, and signaling. It localizes to focal adhesions, dynamic structures that mediate interactions between the extracellular matrix (ECM) and the actin cytoskeleton. PXN serves as a scaffold, integrating signals from integrins, growth factor receptors, and cytoskeletal regulators. Its structure includes multiple protein-binding domains, such as LD motifs and LIM domains, which enable interactions with kinases (e.g., FAK, Src), phosphatases, and cytoskeletal components. These interactions regulate cell spreading, motility, and mechanotransduction, making PXN critical in processes like wound healing, embryonic development, and cancer metastasis.
Recombinant PXN proteins are engineered in vitro using expression systems like *E. coli* or mammalian cells, ensuring high purity and consistency for experimental use. Researchers utilize recombinant PXN to study its phosphorylation dynamics, binding partners, and role in signaling pathways. For example, tyrosine phosphorylation of PXN (e.g., at Y31/Y118) is linked to enhanced cell migration and invasion in cancer models. Additionally, recombinant PXN aids in structural studies, drug screening, and developing inhibitors targeting focal adhesion signaling in diseases.
The development of recombinant PXN has advanced understanding of cellular mechanobiology and pathological conditions. Dysregulation of PXN expression or activity correlates with tumor progression, fibrosis, and cardiovascular disorders. Its recombinant form enables precise manipulation in vitro, overcoming challenges associated with endogenous protein isolation. By providing a standardized tool, recombinant PXN supports reproducible research into cell-ECM interactions and potential therapeutic strategies. However, functional differences between recombinant and native PXN, such as post-translational modifications, require careful interpretation in experimental settings.
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