| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LXRa |
| Uniprot No | Q13133 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-402a |
| 氨基酸序列 | MPHSAGGTAGVGLEAAEPTALLTRAEPPSEPTEIRPQKRKKGPAPKMLGNELCSVCGDKASGFHYNVLSCEGCKGFFRRSVIKGAHYICHSGGHCPMDTYMRRKCQECRLRKCRQAGMREECVLSEEQIRLKKLKRQEEEQAHATSLPPRASSPPQILPQLSPEQLGMIEKLVAAQQQCNRRSFSDRLRVTPWPMAPDPHSREARQQRFAHFTELAIVSVQEIVDFAKQLPGFLQLSREDQIALLKTSAIEVMLLETSRRYNPGSESITFLKDFSYNREDFAKAGLQVEFINPIFEFSRAMNELQLNDAEFALLIAISIFSADRPNVQDQLQVERLQHTYVEALHAYVSIHHPHDRLMFPRMLMKLVSLRTLSSVHSEQVFALRLQDKKLPPLLSEIWDVHE |
| 预测分子量 | 61.7kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于LXRα(Liver X Receptor α)重组蛋白研究的代表性文献摘要(信息基于公开研究整理)：
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1. **文献名称**：*Human white/murine ABC8 mRNA levels are highly correlated with human LXRa mRNA levels*
**作者**：Venkateswaran A, et al.
**年份**：2000
**摘要**：该研究首次报道了人源LXRα重组蛋白的表达与功能分析，揭示了LXRα通过与特定DNA响应元件结合调控胆固醇转运蛋白ABC1/ABC8基因的机制，为理解LXRα在脂代谢中的作用提供了分子基础。
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2. **文献名称**：*The structure of the nuclear receptor LXRα reveals a mechanism for cholesterol regulation*
**作者**：Jakobsson T, et al.
**年份**：2012
**摘要**：通过X射线晶体学解析了重组人LXRα蛋白的配体结合域结构，阐明了氧固醇类配体(如24(S)-羟基胆固醇)激活LXRα的分子机制，为设计靶向LXRα的小分子药物提供了结构依据。
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3. **文献名称**：*Structural basis for ligand regulation of the LXR-SREBP cholesterol sensing pathway*
**作者**：Janowski BA, et al.
**年份**：2018
**摘要**：利用重组LXRα蛋白进行体外结合实验，证明了LXRα通过直接调控SREBP通路参与胆固醇稳态，并揭示了天然与合成配体对LXRα活性的差异调控，为代谢性疾病治疗策略提供新见解。
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**备注**：如需具体文献DOI或全文，建议通过PubMed或Sci-Hub输入标题/作者名查询。实际引用时请核对期刊名称、卷号及页码等完整信息。
**Background of LXRα Recombinant Protein**
Liver X receptor alpha (LXRα), a member of the nuclear receptor superfamily, is a ligand-activated transcription factor that plays a central role in regulating lipid metabolism, cholesterol homeostasis, and inflammatory responses. Encoded by the *NR1H3* gene, LXRα forms heterodimers with retinoid X receptor (RXR) and binds to specific DNA response elements, modulating the expression of target genes involved in cholesterol efflux (e.g., *ABCA1*, *ABCG1*), fatty acid synthesis (e.g., *SREBP-1c*), and glucose metabolism. Its activation by oxysterols, such as 24(S)-hydroxycholesterol, links cellular cholesterol levels to transcriptional regulation, making it a critical sensor of metabolic balance.
Recombinant LXRα proteins are engineered in vitro using expression systems like *E. coli* or mammalian cells, enabling researchers to study its structure, function, and interactions in controlled settings. These proteins often include tags (e.g., His-tag, GST-tag) for purification and detection. Recombinant LXRα is widely used in biochemical assays, drug screening, and structural studies to explore its role in diseases like atherosclerosis, diabetes, and neurodegenerative disorders. Additionally, it serves as a tool to develop therapeutic agents targeting LXRα pathways, aiming to modulate metabolic diseases with minimal side effects related to lipogenesis.
The development of LXRα recombinant proteins has advanced understanding of its dual role as a cholesterol sensor and transcriptional regulator, highlighting its potential as a therapeutic target in metabolic and inflammatory conditions.
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