| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LAMC1 |
| Uniprot No | O43752 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 5-234aa |
| 氨基酸序列 | DPFFVVKGEVQKAVNTAQGLFQRWTELLQDPSTATREEIDWTTNELRNNLRSIEWDLEDLDETISIVEANPRKFNLDATELSIRKAFITSTRQVVRDMKDQMSTSSVQALAERKNRQALLGDSGSQNWSTGTTDKYGRLDRELQRANSHFIEEQQAQQQLIVEQQDEQLELVSGSIGVLKNMSQRIGGELEEQAVMLEDFSHELESTQSRLDNVMKKLAKVSHMTSDRRQ |
| 预测分子量 | 53.4kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于LAMC1重组蛋白的3篇示例参考文献(注：文献信息为虚构示例，建议通过PubMed或Google Scholar检索真实文献)：
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1. **文献名称**: *"Recombinant LAMC1 promotes tumor cell invasion via integrin-mediated signaling pathways"*
**作者**: Zhang Y, et al.
**摘要**: 本研究通过重组表达LAMC1蛋白，发现其能显著增强多种癌细胞系的迁移和侵袭能力。实验表明，LAMC1通过激活整合素α6β4信号通路，促进基质金属蛋白酶(MMPs)的分泌，为肿瘤转移的分子机制提供了新见解。
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2. **文献名称**: *"Expression and functional characterization of human laminin γ1 (LAMC1) in a mammalian cell system"*
**作者**: Smith J, et al.
**摘要**: 作者利用HEK293细胞成功表达并纯化了功能性重组人LAMC1蛋白。该蛋白在体外支持干细胞附着和分化，证实其在组织工程中的应用潜力，为再生医学研究提供了重要工具。
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3. **文献名称**: *"LAMC1 mutations disrupt basement membrane assembly and cause developmental defects in a recombinant model"*
**作者**: Tanaka K, et al.
**摘要**: 通过构建携带LAMC1突变的重组小鼠模型，研究发现LAMC1缺失导致基底膜结构异常，引发胚胎发育缺陷，强调了LAMC1在胚胎形成和细胞外基质稳态中的关键作用。
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如需真实文献，建议使用关键词 **"recombinant LAMC1 protein"** 或 **"LAMC1 laminin"** 在以下平台检索：
- **PubMed**: https://pubmed.ncbi.nlm.nih.gov
- **Google Scholar**: https://scholar.google.com
**Background of LAMC1 Recombinant Protein**
LAMC1. or laminin subunit gamma-1. is a critical component of laminins—a family of extracellular matrix (ECM) glycoproteins essential for tissue structure and cellular signaling. Laminins are heterotrimeric proteins composed of alpha, beta, and gamma chains, with LAMC1 representing the gamma-1 chain. These molecules play pivotal roles in cell adhesion, migration, differentiation, and tissue development by interacting with cell surface receptors like integrins and dystroglycans.
The LAMC1-containing laminins, particularly laminin-511 (α5β1γ1) and laminin-521 (α5β2γ1), are vital during embryogenesis and organogenesis. They are also involved in maintaining epithelial and endothelial basement membranes. Dysregulation of LAMC1 expression has been linked to pathologies, including cancer metastasis, fibrosis, and skin disorders, highlighting its biomedical relevance.
Recombinant LAMC1 protein is engineered in vitro using expression systems such as mammalian cells (e.g., HEK293) to ensure proper post-translational modifications. This recombinant form retains functional domains required for ECM interactions, enabling researchers to study laminin-mediated processes in controlled settings. Applications include investigating cell-ECM dynamics, modeling diseases *in vitro*, and developing biomimetic scaffolds for tissue engineering. Additionally, recombinant LAMC1 serves as a standardized tool for drug screening or antibody production, circumventing variability from native sources. Its use has advanced understanding of ECM biology and therapeutic targeting in regenerative medicine and oncology.
In summary, LAMC1 recombinant protein provides a versatile, high-purity resource for exploring laminin functions and their implications in health and disease.
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