| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LAMa3 |
| Uniprot No | Q16363 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 全长 |
| 氨基酸序列 | full |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于LAMa3重组蛋白相关研究方向的模拟参考文献示例(非真实文献，仅供格式参考)：
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1. **标题**：Recombinant Laminin α3 Chain Expression in Mammalian Cells and Its Role in Epithelial Adhesion
**作者**：Smith J, et al.
**摘要**：研究利用HEK293细胞成功表达重组LAMa3蛋白，并验证其与整合素结合的能力，证实其在体外促进表皮细胞黏附的功能。
2. **标题**：Functional Characterization of LAMa3 in Basement Membrane Assembly Using Recombinant Fragments
**作者**：Lee H, et al.
**摘要**：通过重组LAMa3片段解析其结构域在基底膜组装中的作用，发现特定区域对胶原IV和细胞受体结合至关重要。
3. **标题**：Therapeutic Potential of Recombinant LAMa3 for Junctional Epidermolysis Bullosa
**作者**：Cheng R, et al.
**摘要**：在小鼠模型中验证重组LAMa3蛋白修复皮肤基底膜缺陷的效果，为遗传性大疱性表皮松解症提供潜在治疗策略。
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**提示**：实际文献请通过 **PubMed/Google Scholar** 检索关键词：`recombinant Laminin alpha3` 或 `LAMA3 protein expression`，并筛选近年高相关度论文。
**Background of LAMa3 Recombinant Protein**
Laminin α3 (LAMa3), a subunit of the heterotrimeric laminin-332 protein (α3β3γ2), is a critical component of the extracellular matrix (ECM). Laminins are glycoproteins essential for basement membrane assembly, cell adhesion, differentiation, and tissue integrity. LAMa3. encoded by the *LAMA3* gene, plays a pivotal role in epithelial-mesenchymal interactions, particularly in skin, kidney, and lung tissues.
Laminin-332. formed by LAMa3. β3. and γ2 chains, is a key mediator of epidermal-dermal adhesion in the skin. Mutations in *LAMA3* are linked to junctional epidermolysis bullosa (JEB), a severe genetic disorder characterized by fragile skin and mucosal blistering. This underscores LAMa3's importance in maintaining structural stability at the dermal-epidermal junction.
Recombinant LAMa3 is produced using biotechnological systems (e.g., mammalian cell cultures or insect cells) to express the purified protein for research and therapeutic applications. Its recombinant form retains functional domains, including binding sites for integrins and other ECM components, enabling studies on cell migration, wound healing, and tissue regeneration.
In regenerative medicine, LAMa3 recombinant protein is explored for skin grafts, artificial skin models, and treating chronic wounds. It also serves as a tool to investigate cancer metastasis, as laminin-332 influences tumor cell invasion and signaling. By mimicking native ECM interactions, recombinant LAMa3 advances our understanding of developmental biology, disease mechanisms, and potential therapies for ECM-related disorders.
Overall, LAMa3 recombinant protein bridges basic research and clinical innovation, offering insights into tissue repair and targeted therapeutic strategies.
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