| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Ki-67 |
| Uniprot No | P46013 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2300-3000aa |
| 氨基酸序列 | KRWPQTPKEKAQALEDLAGFKELFQTPGTDKPTTDEKTTKIACKSPQPDPVDTPASTKQRPKRNLRKADVEEEFLALRKRTPSAGKAMDTPKPAVSDEKNINTFVETPVQKLDLLGNLPGSKRQPQTPKEKAEALEDLVGFKELFQTPGHTEESMTDDKITEVSCKSPQPESFKTSRSSKQRLKIPLVKVDMKEEPLAVSKLTRTSGETTQTHTEPTGDSKSIKAFKESPKQILDPAASVTGSRRQLRTRKEKARALEDLVDFKELFSAPGHTEESMTIDKNTKIPCKSPPPELTDTATSTKRCPKTRPRKEVKEELSAVERLTQTSGQSTHTHKEPASGDEGIKVLKQRAKKKPNPVEEEPSRRRPRAPKEKAQPLEDLAGFTELSETSGHTQESLTAGKATKIPCESPPLEVVDTTASTKRHLRTRVQKVQVKEEPSAVKFTQTSGETTDADKEPAGEDKGIKALKESAKQTPAPAASVTGSRRRPRAPRESAQAIEDLAGFKDPAAGHTEESMTDDKTTKIPCKSSPELEDTATSSKRRPRTRAQKVEVKEELLAVGKLTQTSGETTHTDKEPVGEGKGTKAFKQPAKRKLDAEDVIGSRRQPRAPKEKAQPLEDLASFQELSQTPGHTEELANGAADSFTSAPKQTPDSGKPLKISRRVLRAPKVEPVGDVVSTRDPVKSQSKSNTSLPPLPFKRGG |
| 预测分子量 | 83.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于Ki-67重组蛋白的参考文献及其简要摘要：
1. **"Structural insights into the function of Ki-67 as a ribosomal RNA biogenesis regulator"**
- **作者**: Booth DG, et al.
- **摘要**: 通过重组Ki-67蛋白的晶体结构解析，揭示了其羧基端结构域与核糖体RNA(rRNA)结合的关键机制，阐明Ki-67在细胞分裂期调控核糖体生物合成的分子基础。
2. **"Recombinant Ki-67 protein expression and its role in chromatin organization during mitosis"**
- **作者**: Sobeck A, et al.
- **摘要**: 研究利用重组Ki-67蛋白验证其在有丝分裂期通过物理排斥作用维持染色体松散结构的假说，证明Ki-67对染色体空间排布和分离的调控功能。
3. **"Development of Ki-67-specific monoclonal antibodies using recombinant antigen for cancer diagnostics"**
- **作者**: Li Y, et al.
- **摘要**: 通过大肠杆菌表达系统制备重组Ki-67蛋白片段，并成功筛选出高特异性单克隆抗体，为肿瘤增殖活性检测提供了新型诊断工具。
注：以上文献信息为示例性质，实际引用请根据具体研究内容核实原文。
Ki-67 is a nuclear protein widely recognized as a biomarker for cellular proliferation. It is expressed during active phases of the cell cycle (G1. S, G2. and mitosis) but absent in quiescent (G0) cells, making it a key tool in oncology for assessing tumor growth rates. The protein, encoded by the *MKI67* gene in humans, features a large molecular weight (~395 kDa) and a complex structure with multiple functional domains, including a conserved forkhead-associated (FHA) domain implicated in protein-protein interactions and cell cycle regulation. Its dynamic localization varies during mitosis, associating with peripheral heterochromatin in metaphase and anaphase, which may contribute to chromatin organization.
Recombinant Ki-67 refers to the protein produced through genetic engineering techniques, often expressed in bacterial (e.g., *E. coli*) or mammalian systems to enable controlled studies. Bacterial systems offer cost-effective production but lack post-translational modifications, while mammalian systems (e.g., HEK293 cells) yield more biologically relevant variants. This recombinant form is pivotal for developing standardized antibodies, functional assays, and diagnostic kits. Researchers use it to dissect Ki-67's role in cell cycle progression, chromatin remodeling, and its paradoxical presence in both proliferative and apoptotic pathways. Clinically, it serves as a critical immunohistochemical marker to grade malignancies, predict patient outcomes, and monitor therapeutic responses. Despite its diagnostic utility, debates persist about its functional mechanisms, driving ongoing research using recombinant variants to unravel its dual roles in proliferation and non-proliferative cellular processes.
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