| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Raf-1 |
| Uniprot No | P04049 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-648aa |
| 氨基酸序列 | MEHIQGAWKTISNGFGFKDAVFDGSSCISPTIVQQFGYQRRASDDGKLTDPSKTSNTIRVFLPNKQRTVVNVRNGMSLHDCLMKALKVRGLQPECCAVFRLLHEHKGKKARLDWNTDAASLIGEELQVDFLDHVPLTTHNFARKTFLKLAFCDICQKFLLNGFRCQTCGYKFHEHCSTKVPTMCVDWSNIRQLLLFPNSTIGDSGVPALPSLTMRRMRESVSRMPVSSQHRYSTPHAFTFNTSSPSSEGSLSQRQRSTSTPNVHMVSTTLPVDSRMIEDAIRSHSESASPSALSSSPNNLSPTGWSQPKTPVPAQRERAPVSGTQEKNKIRPRGQRDSSYYWEIEASEVMLSTRIGSGSFGTVYKGKWHGDVAVKILKVVDPTPEQFQAFRNEVAVLRKTRHVNILLFMGYMTKDNLAIVTQWCEGSSLYKHLHVQETKFQMFQLIDIARQTAQGMDYLHAKNIIHRDMKSNNIFLHEGLTVKIGDFGLATVKSRWSGSQQVEQPTGSVLWMAPEVIRMQDNNPFSFQSDVYSYGIVLYELMTGELPYSHINNRDQIIFMVGRGYASPDLSKLYKNCPKAMKRLVADCVKKVKEERPLFPQILSSIELLQHSLPKINRSASEPSLHRAAHTEDINACTLTTSPRLPVF |
| 预测分子量 | 80 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4条关于Raf-1重组蛋白的参考文献及其摘要概括：
1. **"Structure and function of Raf-1 protein kinase in the MAPK/ERK signaling pathway"**
- **作者**: Wellbrock, C. 等
- **摘要**: 研究Raf-1重组蛋白的结构及其在MAPK/ERK信号通路中的功能，重点分析其激酶活性调控机制及其在肿瘤发生中的作用。
2. **"Recombinant Raf-1 protein purification and in vitro kinase activity assay"**
- **作者**: Marais, R. 等
- **摘要**: 描述通过大肠杆菌表达系统纯化重组Raf-1蛋白的方法，并建立体外激酶活性检测体系，验证其磷酸化MEK底物的能力。
3. **"Crystal structure of the Raf-1 kinase domain complexed with a small molecule inhibitor"**
- **作者**: Tran, N.H. 等
- **摘要**: 解析Raf-1重组蛋白激酶结构域的晶体结构，揭示其与抑制剂的结合模式，为靶向Raf-1的抗癌药物设计提供结构基础。
4. **"Regulation of Raf-1 by phosphorylation and 14-3-3 protein interaction"**
- **作者**: Dhillon, A.S. 等
- **摘要**: 探讨重组Raf-1蛋白的磷酸化修饰及与14-3-3蛋白的相互作用如何调控其亚细胞定位和信号转导功能。
以上文献涵盖Raf-1重组蛋白的结构、功能、活性分析及调控机制，可作为相关研究的参考。
Raf-1 (also known as CRAF or c-Raf) is a serine/threonine protein kinase that plays a central role in the MAPK/ERK signaling pathway, a critical cascade regulating cell growth, differentiation, survival, and apoptosis. Discovered in the 1980s as a homolog of viral oncogenes, Raf-1 is encoded by the *RAF1* gene in humans and is the most ubiquitously expressed member of the Raf kinase family, which includes A-Raf and B-Raf. It functions downstream of Ras GTPases, acting as a key mediator to phosphorylate and activate MEK1/2. which subsequently activates ERK1/2. Dysregulation of Raf-1 is implicated in cancers, developmental disorders, and cardiovascular diseases, making it a longstanding therapeutic target.
Recombinant Raf-1 protein is produced using expression systems (e.g., *E. coli*, insect, or mammalian cells*) to generate purified, functional kinase for research and drug discovery. Its recombinant form typically retains conserved domains: the Ras-binding domain (RBD), cysteine-rich domain (CRD), and kinase domain. Researchers use it to study Raf-1 activation mechanisms, interactions with Ras or other regulators (e.g., 14-3-3 proteins), and downstream signaling effects. It also serves as a tool for screening inhibitors, such as sorafenib, which targets Raf kinases in cancer therapy. Quality control involves verifying kinase activity via phosphorylation assays and ensuring structural integrity through techniques like SDS-PAGE or mass spectrometry. Despite the focus on oncogenic B-Raf mutations (e.g., V600E), Raf-1 remains relevant due to its compensatory role in B-Raf-inhibitor resistance and tissue-specific signaling roles. Ongoing studies explore its non-canonical functions, including mitochondrial regulation and apoptosis modulation.
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