| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | COL11a1 |
| Uniprot No | P12107 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 532-699aa |
| 氨基酸序列 | GPMGLTGRPGPVGGPGSSGAKGESGDPGPQGPRGVQGPPGPTGKPGKRGRPGADGGRGMPGEPGAKGDRGFDGLPGLPGDKGHRGERGPQGPPGPPGDDGMRGEDGEIGPRGLPGEAGPRGLLGPRGTPGAPGQPGMAGVDGPPGPKGNMGPQGEPGPPGQQGNPGPQ |
| 预测分子量 | 47.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于COL11A1重组蛋白的3篇参考文献示例(内容基于公开研究综合概括，非真实文献)：
1. **文献名称**: "Recombinant COL11A1 production in mammalian cells and its role in extracellular matrix assembly"
**作者**: Smith J. et al.
**摘要**: 研究利用哺乳动物表达系统成功表达并纯化重组COL11A1蛋白，证实其与胶原V、XI的相互作用，揭示其在胞外基质纤维形成中的关键调控作用。
2. **文献名称**: "Structural characterization of COL11A1 fibrillar domains and implications for fibrosis"
**作者**: Lee H. et al.
**摘要**: 通过大肠杆菌重组表达COL11A1的特定结构域，结合X射线晶体学分析其三维结构，发现其N端结构域可能通过TGF-β通路促进纤维化疾病进展。
3. **文献名称**: "COL11A1 recombinant protein enhances cancer cell invasion via integrin signaling"
**作者**: Chen R. et al.
**摘要**: 研究重组COL11A1蛋白在肿瘤微环境中的作用，发现其通过激活整合素α2β1信号通路促进癌细胞迁移和侵袭，为靶向治疗提供依据。
注：以上为模拟文献，实际引用时需查询具体数据库(如PubMed)获取真实研究。
**Background of COL11A1 Recombinant Protein**
COL11A1 (Collagen Type XI Alpha 1 Chain) is a critical component of extracellular matrices, primarily forming heterotypic fibrils with collagen types II and XI to regulate fibril diameter and organization in connective tissues. It is encoded by the *COL11A1* gene, located on human chromosome 1p21. Structurally, it consists of a triple-helical domain flanked by non-collagenous regions, facilitating interactions with other matrix proteins.
COL11A1 plays essential roles in embryonic development, skeletal morphogenesis, and tissue integrity. Mutations in *COL11A1* are linked to genetic disorders like Marshall syndrome, Stickler syndrome type 2. and fibrochondrogenesis, characterized by craniofacial abnormalities, hearing loss, and skeletal defects. These conditions underscore its importance in collagen fibril assembly and tissue-specific biomechanical functions.
Recombinant COL11A1 protein is engineered using heterologous expression systems (e.g., mammalian HEK293 or CHO cells) to ensure proper post-translational modifications, such as hydroxylation and glycosylation. This protein serves as a tool to study collagen interactions, disease mechanisms, and therapeutic strategies. Applications include *in vitro* modeling of cartilage development, screening for molecular therapies targeting collagenopathies, and tissue engineering for regenerative medicine. Its recombinant form also aids in elucidating structure-function relationships and validating pathogenic mutations in clinical diagnostics.
Research on COL11A1 recombinant protein continues to advance insights into connective tissue disorders and potential interventions.
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