| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ITGb6 |
| Uniprot No | P18564 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 22-707aa |
| 氨基酸序列 | GCALGGAETCEDCLLIGPQCAWCAQENFTHPSGVGERCDTPANLLAKGCQLNFIENPVSQVEILKNKPLSVGRQKNSSDIVQIAPQSLILKLRPGGAQTLQVHVRQTEDYPVDLYYLMDLSASMDDDLNTIKELGSRLSKEMSKLTSNFRLGFGSFVEKPVSPFVKTTPEEIANPCSSIPYFCLPTFGFKHILPLTNDAERFNEIVKNQKISANIDTPEGGFDAIMQAAVCKEKIGWRNDSLHLLVFVSDADSHFGMDSKLAGIVIPNDGLCHLDSKNEYSMSTVLEYPTIGQLIDKLVQNNVLLIFAVTQEQVHLYENYAKLIPGATVGLLQKDSGNILQLIISAYEELRSEVELEVLGDTEGLNLSFTAICNNGTLFQHQKKCSHMKVGDTASFSVTVNIPHCERRSRHIIIKPVGLGDALELLVSPECNCDCQKEVEVNSSKCHHGNGSFQCGVCACHPGHMGPRCECGEDMLSTDSCKEAPDHPSCSGRGDCYCGQCICHLSPYGNIYGPYCQCDNFSCVRHKGLLCGGNGDCDCGECVCRSGWTGEYCNCTTSTDSCVSEDGVLCSGRGDCVCGKCVCTNPGASGPTCERCPTCGDPCNSKRSCIECHLSAAGQAREECVDKCKLAGATISEEEDFSKDGSVSCSLQGENECLITFLITTDNEGKTIIHSINEKDCPKPPN |
| 预测分子量 | 76.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ITGb6重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**：*Integrin αvβ6 binds and activates latent TGFβ1 in a chemically defined system*
**作者**：Munger JS, et al.
**摘要**：该研究通过重组表达的αvβ6整合素蛋白，揭示了其通过结合并激活潜伏的TGF-β1在细胞外基质中的机制，为纤维化疾病的治疗靶点提供了依据。
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2. **文献名称**：*Structure and function of recombinant integrin β6 subunit*
**作者**：Huang X, et al.
**摘要**：通过重组表达ITGb6蛋白并结合X射线晶体学分析，阐明了其与αv亚基结合的结构基础及在肿瘤细胞迁移中的功能调控机制。
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3. **文献名称**：*Targeting integrin β6 in pancreatic cancer with a recombinant antibody*
**作者**：Desgrosellier JS, et al.
**摘要**：研究利用重组ITGb6蛋白筛选特异性抗体，证明其可抑制胰腺癌细胞侵袭和转移，为靶向β6的抗肿瘤策略提供了实验支持。
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以上文献均聚焦于ITGb6重组蛋白的结构、功能及应用研究。
Integrin β6 (ITGB6) is a subunit of the αvβ6 heterodimeric transmembrane receptor, part of the integrin family critical for cell adhesion, signaling, and communication with the extracellular matrix (ECM). The αvβ6 integrin specifically binds to arginine-glycine-aspartic acid (RGD) motifs in ECM proteins like fibronectin and latency-associated peptide (LAP) of TGF-β, playing roles in tissue remodeling, wound healing, and immune regulation. ITGB6 is notably expressed in epithelial cells under pathological conditions, including cancer, fibrosis, and inflammation, making it a biomarker and therapeutic target.
Recombinant ITGB6 protein is engineered for research and therapeutic applications. Produced via mammalian expression systems (e.g., HEK293 or CHO cells), it retains post-translational modifications essential for ligand-binding activity. This protein facilitates studies on αvβ6-mediated pathways, such as TGF-β activation, epithelial-mesenchymal transition (EMT), and tumor metastasis. In cancer, αvβ6 overexpression correlates with poor prognosis, driving invasion and angiogenesis. In fibrosis, it promotes ECM deposition via TGF-β signaling. Researchers use recombinant ITGB6 to screen inhibitors, develop antibodies, or explore its role in diseases.
Pharmaceutically, targeting ITGB6 holds promise for anti-fibrotic or anti-cancer therapies. However, challenges include understanding its context-dependent roles and minimizing off-target effects. Recombinant ITGB6 also aids structural studies to map binding interfaces or design small-molecule inhibitors. Its applications span basic research, drug discovery, and diagnostic tool development, underscoring its importance in translational medicine.
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