| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ITGa11 |
| Uniprot No | Q9UKX5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1188aa |
| 氨基酸序列 | MDLPRGLVVAWALSLWPGFTDTFNMDTRKPRVIPGSRTAFFGYTVQQHDISGNKWLVVGAPLETNGYQKTGDVYKCPVIHGNCTKLNLGRVTLSNVSERKDNMRLGLSLATNPKDNSFLACSPLWSHECGSSYYTTGMCSRVNSNFRFSKTVAPALQRCQTYMDIVIVLDGSNSIYPWVEVQHFLINILKKFYIGPGQIQVGVVQYGEDVVHEFHLNDYRSVKDVVEAASHIEQRGGTETRTAFGIEFARSEAFQKGGRKGAKKVMIVITDGESHDSPDLEKVIQQSERDNVTRYAVAVLGYYNRRGINPETFLNEIKYIASDPDDKHFFNVTDEAALKDIVDALGDRIFSLEGTNKNETSFGLEMSQTGFSSHVVEDGVLLGAVGAYDWNGAVLKETSAGKVIPLRESYLKEFPEELKNHGAYLGYTVTSVVSSRQGRVYVAGAPRFNHTGKVILFTMHNNRSLTIHQAMRGQQIGSYFGSEITSVDIDGDGVTDVLLVGAPMYFNEGRERGKVYVYELRQNLFVYNGTLKDSHSYQNARFGSSIASVRDLNQDSYNDVVVGAPLEDNHAGAIYIFHGFRGSILKTPKQRITASELATGLQYFGCSIHGQLDLNEDGLIDLAVGALGNAVILWSRPVVQINASLHFEPSKINIFHRDCKRSGRDATCLAAFLCFTPIFLAPHFQTTTVGIRYNATMDERRYTPRAHLDEGGDRFTNRAVLLSSGQELCERINFHVLDTADYVKPVTFSVEYSLEDPDHGPMLDDGWPTTLRVSVPFWNGCNEDEHCVPDLVLDARSDLPTAMEYCQRVLRKPAQDCSAYTLSFDTTVFIIESTRQRVAVEATLENRGENAYSTVLNISQSANLQFASLIQKEDSDGSIECVNEERRLQKQVCNVSYPFFRAKAKVAFRLDFEFSKSIFLHHLEIELAAGSDSNERDSTKEDNVAPLRFHLKYEADVLFTRSSSLSHYEVKPNSSLERYDGIGPPFSCIFRIQNLGLFPIHGMMMKITIPIATRSGNRLLKLRDFLTDEANTSCNIWGNSTEYRPTPVEEDLRRAPQLNHSNSDVVSINCNIRLVPNQEINFHLLGNLWLRSLKALKYKSMKIMVNAALQRQFHSPFIFREEDPSRQIVFEISKQEDWQVPIWIIVGSTLGGLLLLALLVLALWKLGFFRSARRRREPGLDPTPKVLE |
| 预测分子量 | 133,4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ITGA11重组蛋白的3篇假设性参考文献示例(注：实际文献需通过学术数据库查询)：
1. **"Integrin α11β1 regulates collagen reorganization in tumor stroma"**
*作者：Helga E. B. et al. (2022)*
**摘要**：研究ITGA11重组蛋白在肿瘤微环境中的作用，发现其通过结合胶原蛋白调控肿瘤相关成纤维细胞(CAFs)的机械信号传导，促进胶原纤维排列并影响癌细胞侵袭。
2. **"Structural insights into the collagen-binding mechanism of integrin α11"**
*作者：Zhang Y. et al. (2021)*
**摘要**：通过冷冻电镜解析ITGA11重组蛋白的胞外域结构，揭示其与I型胶原特异性结合的分子机制，为靶向ITGA11的药物设计提供结构基础。
3. **"ITGA11 knockdown attenuates TGF-β-induced pulmonary fibrosis"**
*作者：Suzuki K. et al. (2020)*
**摘要**：在小鼠模型中证实ITGA11重组蛋白参与TGF-β信号通路，沉默ITGA11可减少肌成纤维细胞分化及细胞外基质沉积，提示其作为肺纤维化治疗靶点。
建议通过PubMed、Google Scholar等平台检索真实文献，关键词：**"integrin alpha 11 recombinant" / "ITGA11 collagen" / "ITGA11 disease"**。
**Background of ITGa11 Recombinant Protein**
Integrin alpha-11 (ITGa11) is a key subunit of the integrin family of transmembrane receptors, which mediate cell-cell and cell-extracellular matrix (ECM) interactions. As part of the α-subunit family, ITGa11 pairs with β1-integrin to form the collagen-binding receptor α11β1 integrin. This heterodimer specifically recognizes collagen types I, II, and IV, playing critical roles in cellular adhesion, migration, and signaling during tissue development, homeostasis, and repair.
ITGa11 is predominantly expressed in mesenchymal cells, such as fibroblasts and smooth muscle cells, and is essential for regulating collagen fibril organization and mechanical signaling. Its function extends to modulating cell proliferation, differentiation, and ECM remodeling. Dysregulation of ITGa11 has been implicated in pathological conditions, including fibrosis, cancer progression, and cardiovascular diseases. For instance, in tumor microenvironments, ITGa11 influences cancer cell invasion and metastasis by mediating interactions with collagen-rich matrices.
Recombinant ITGa11 protein is engineered for research and therapeutic applications. Produced via heterologous expression systems (e.g., mammalian or insect cells), it retains functional domains required for ligand binding and signaling. Researchers utilize this protein to study integrin-mediated pathways, screen for inhibitors or agonists, and develop targeted therapies. Its applications also include structural studies to elucidate binding mechanisms with collagen and downstream signaling partners.
Recent studies highlight ITGa11's dual role in tissue regeneration and disease, making it a promising biomarker or therapeutic target. However, its complex regulation and context-dependent functions necessitate further exploration. Recombinant ITGa11 thus serves as a vital tool for advancing understanding of integrin biology and its translational potential in medicine.
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