| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LAMC2 |
| Uniprot No | Q13753 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 417-588aa |
| 氨基酸序列 | NCQGGGACDPDTGDCYSGDENPDIECADCPIGFYNDPHDPRSCKPCPCHNGFSCSVMPETEEVVCNNCPPGVTGARCELCADGYFGDPFGEHGPVRPCQPCQCNNNVDPSASGNCDRLTGRCLKCIHNTAGIYCDQCKAGYFGDPLAPNPADKCRACNCNPMGSEPVGCRSD |
| 预测分子量 | 19.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于LAMC2重组蛋白的3篇参考文献及其简要摘要：
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1. **文献名称**: *Laminin gamma 2 mediates tumor cell invasion via aberrant expression and activation of matrix metalloproteinases*
**作者**: Koshikawa N, et al.
**摘要**: 该研究通过重组LAMC2蛋白实验，揭示了其在肿瘤细胞侵袭中的关键作用，表明LAMC2通过异常激活基质金属蛋白酶(MMPs)促进细胞外基质降解，从而增强癌细胞转移能力。
2. **文献名称**: *Recombinant laminin gamma2 chain fragment (LG4-5) modulates integrin signaling to promote wound healing*
**作者**: Nishiuchi R, et al.
**摘要**: 研究利用重组LAMC2的LG4-5片段，证明其通过整合素信号通路促进细胞粘附和迁移，为基于LAMC2的重组蛋白在组织修复中的应用提供理论支持。
3. **文献名称**: *LAMC2 as a prognostic biomarker in gastric cancer: Recombinant protein-based functional validation*
**作者**: Zhao Y, et al.
**摘要**: 通过重组LAMC2蛋白体外实验，验证了其在胃癌细胞中通过激活PI3K/AKT通路促进增殖和转移的机制，提示其作为治疗靶点和预后标志物的潜力。
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以上文献均围绕LAMC2重组蛋白的功能研究，涵盖肿瘤转移、信号通路及临床应用方向。如需更多文献或具体细节，建议通过PubMed或Web of Science进一步检索。
LAMC2 (laminin gamma-2 chain) is a critical component of laminin-332 (formerly laminin-5), a heterotrimeric extracellular matrix (ECM) protein composed of α3. β3. and γ2 chains. As a member of the laminin family, it plays a foundational role in maintaining epithelial integrity by forming structural networks at the basement membrane. Recombinant LAMC2 is engineered to study its biological functions or therapeutic potential, often expressed in mammalian or insect cell systems to ensure proper post-translational modifications.
Laminin-332 is essential for cell adhesion, migration, and tissue organization. The γ2 chain (LAMC2) contributes to the protein’s integrin-binding capacity, facilitating interactions with cellular receptors like α6β4 and α3β1 integrins. These interactions activate signaling pathways regulating proliferation, differentiation, and wound healing. Dysregulation of LAMC2 is implicated in pathological processes, including cancer metastasis and chronic wounds. For example, LAMC2 overexpression in carcinomas promotes tumor invasion by enhancing ECM remodeling and cell motility.
Recombinant LAMC2 is widely used to model basement membrane dynamics in vitro, particularly in 3D cell cultures or organoid systems. It also serves as a tool to investigate molecular mechanisms underlying genetic disorders, such as junctional epidermolysis bullosa, where LAMC2 mutations cause skin fragility. Additionally, its role in tissue repair has spurred interest in bioengineering applications, such as designing synthetic matrices for regenerative medicine.
The production of recombinant LAMC2 addresses challenges in isolating native proteins from tissues, offering scalable, high-purity alternatives. Its characterization includes functional assays (e.g., cell adhesion, migration studies) and structural analyses to confirm trimer formation. Ongoing research aims to harness LAMC2’s ECM-modulating properties for targeted therapies, including anti-metastatic agents or bioactive coatings for medical implants.
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