| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PCDHb16 |
| Uniprot No | Q9NRJ7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-776aa |
| 氨基酸序列 | MEIGWMHNRRQRQVLVFFVLLSLSGAGAELGSYSVVEETERGSFVANLGKDLGLGLTEMSTRKARIISQGNKQHLQLKAQTGDLLINEKLDREELCGPTEPCILHFQVLMENPLEIFQAELRVIDINDHSPMFTEKEMILKIPENSPLGTEFPLNHALDLDVGSNNVQNYKISPSSHFRVLIHEFRDGRKYPELVLDKELDREEEPQLRLTLTALDGGSPPRSGTAQVRIEVVDINDNAPEFEQPIYKVQIPENSPLGSLVATVSARDLDGGANGKISYTLFQPSEDISKTLEVNPMTGEVRLRKQVDFEMVTSYEVRIKATDGGGLSGKCTLLLQVVDVNDNPPQVTMSALTSPIPENSPEIVVAVFSVSDPDSGNNGKTISSIQEDLPFLLKPSVKNFYTLVTERALDREARAEYNITLTVTDMGTPRLKTEHNITVQISDVNDNAPTFTQTSYTLFVRENNSPALHIGSVSATDRDSGTNAQVTYSLLPPQDPHLPLASLVSINADNGHLFALRSLDYEALQAFEFRVGATDRGSPALSREALVRVLVLDANDNSPFVLYPLQNGSAPCTELVPRAAEPGYLVTKVVAVDGDSGQNAWLSYQLLKATEPGLFGVWAHNGEVRTARLLSERDAAKQRLVVLVKDNGEPPRSATATLHVLLVDGFSQPFLPLPEAAPGQTQANSLTVYLVVALASVSSLFLFSVLLFVAVRLCRRSRAASVGRCSMPEGPFPGRLVDVSGTGTLSQSYQYEVCLTGGSETSEFKFLKPIIPNFSP |
| 预测分子量 | 84,9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PCDHb16重组蛋白的模拟参考文献示例(实际文献可能需要通过学术数据库进一步检索)：
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1. **文献名称**: *"Recombinant Expression and Purification of Human PCDHb16 in Mammalian Cell Systems"*
**作者**: Chen L., Wang Y., et al.
**摘要**: 本研究报道了在哺乳动物细胞系统中重组表达人源PCDHb16蛋白的方法，通过优化载体设计和转染条件，获得高纯度蛋白。纯化后的蛋白用于研究其细胞表面定位及与其他钙粘蛋白的相互作用。
2. **文献名称**: *"Structural Insights into PCDHb16-Mediated Synaptic Adhesion"*
**作者**: Kim S., Ito H., et al.
**摘要**: 通过重组PCDHb16蛋白的体外功能实验和结构分析，揭示了其胞外结构域在神经元突触粘附中的作用，并发现其依赖钙离子的同源二聚化特性。
3. **文献名称**: *"Functional Role of Recombinant PCDHb16 in Neural Circuit Assembly"*
**作者**: Garcia M., Patel R., et al.
**摘要**: 利用重组PCDHb16蛋白进行体外神经元培养实验，证明其通过调控轴突导向和树突分支参与神经环路组装，可能与自闭症相关基因网络有关。
4. **文献名称**: *"Development of a PCDHb16-Specific Monoclonal Antibody Using Recombinant Protein"*
**作者**: Zhang Q., Li X., et al.
**摘要**: 研究基于大肠杆菌表达的重组PCDHb16蛋白制备单克隆抗体，验证了抗体在免疫组化和Western blot中的特异性，为疾病生物标志物研究提供工具。
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**说明**：以上为模拟参考文献，实际研究中请通过PubMed、Web of Science等平台检索真实文献(关键词如“PCDHb16 recombinant”或“protocadherin beta 16 expression”)。若研究较少，可扩展至原钙粘蛋白β家族的整体重组技术或功能研究。
PCDHb16 (Protocadherin Beta-16) is a member of the clustered protocadherin (PCDH) family, a subgroup of cadherin superfamily proteins known for their roles in cell-cell adhesion, neuronal development, and synaptic specificity. The PCDHβ cluster, to which PCDHb16 belongs, encodes transmembrane proteins characterized by extracellular cadherin repeats, a single-pass transmembrane domain, and cytoplasmic regions that mediate intracellular signaling. These proteins are predominantly expressed in the nervous system and are critical for establishing neural circuit diversity, axon guidance, and neuronal survival through homophilic or heterophilic interactions.
Recombinant PCDHb16 protein is engineered to study its structural and functional properties in vitro. Typically produced using expression systems like Escherichia coli or mammalian cells (e.g., HEK293), the recombinant protein retains key domains, including extracellular cadherin motifs involved in adhesion. Purification often employs affinity tags (e.g., His-tag) followed by chromatography. Researchers utilize this protein to investigate its role in neurodevelopmental processes, such as synapse formation or neuronal migration, and to explore its potential involvement in diseases. For instance, PCDH mutations are linked to neurological disorders (e.g., autism, epilepsy) and cancers, where aberrant cell adhesion promotes metastasis.
Current studies focus on mapping PCDHb16’s binding partners, signaling pathways, and tissue-specific expression patterns. Challenges include maintaining protein solubility and proper folding during production, which may require optimized expression conditions or chaperone co-expression. Recombinant PCDHb16 also serves as an antigen for antibody development or a reagent in cell adhesion assays. Its study contributes to understanding the molecular basis of neural wiring and may inform therapeutic strategies for neurodevelopmental disorders or cancer. However, functional redundancy within the PCDH family and incomplete mechanistic insights necessitate further research to clarify its distinct biological roles.
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