| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PRR4 |
| Uniprot No | Q16378 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 17-134aa |
| 氨基酸序列 | QSTD NDVNYEDFTF TIPDVEDSSQ RPDQGPQRPP PEGLLPRPPG DSGNQDDGPQ QRPPKPGGHH RHPPPPPFQN QQRPPRRGHR QLSLPRFPSV SLQEASSFFQ RDRPARHPQE QPLW |
| 预测分子量 | 15 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与PRR4(Proline Rich Protein 4)重组蛋白相关的文献摘要及作者信息(注：PRR4研究相对较少，以下为模拟示例，实际文献需通过学术数据库检索确认)：
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1. **文献名称**: *"Recombinant PRR4 Protein Attenuates Inflammation in Corneal Epithelial Cells via Modulating TLR2 Signaling"*
**作者**: Smith J, et al.
**摘要**: 本研究成功在大肠杆菌中表达并纯化了重组PRR4蛋白，发现其通过抑制TLR2受体信号通路，显著减少角膜上皮细胞中促炎因子(如IL-6和TNF-α)的释放，提示PRR4在眼部抗炎治疗中的潜在应用。
2. **文献名称**: *"Expression and Functional Characterization of PRR4 Recombinant Protein in Salivary Gland Development"*
**作者**: Lee H, et al.
**摘要**: 通过哺乳动物细胞系表达PRR4重组蛋白，证实其在唾液腺发育中调控细胞黏附和增殖。研究还发现PRR4与β-catenin存在相互作用，可能影响Wnt信号通路活性。
3. **文献名称**: *"Structural Analysis of PRR4 Reveals a Novel Proline-rich Domain Critical for Antimicrobial Activity"*
**作者**: Zhang R, et al.
**摘要**: 利用重组PRR4蛋白进行结构解析，发现其N端富含脯氨酸的结构域可直接结合细菌细胞壁成分(如脂多糖)，并具有广谱抗菌活性，为新型抗菌肽设计提供了理论依据。
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**备注**：
- 以上内容为基于PRR4已知生物学功能的模拟文献(PRR4在唾液、角膜及先天免疫中有报道)。
- 实际文献建议通过PubMed或Google Scholar以关键词 **"PRR4 recombinant"** 或 **"Proline-rich protein 4 expression"** 检索。
**Background of PRR4 Recombinant Protein**
PRR4 (Proline-Rich Protein 4) is a secretory protein predominantly expressed in salivary glands and mucosal tissues. It belongs to the proline-rich protein family, characterized by high proline and glycine content, which contributes to its structural flexibility and functional diversity. PRR4 plays a critical role in maintaining oral and mucosal homeostasis, primarily through its involvement in lubrication, antimicrobial defense, and tissue repair. Its innate ability to bind microbial surfaces and modulate immune responses highlights its importance in protecting against pathogens and promoting mucosal integrity.
Recombinant PRR4 protein is engineered using genetic cloning techniques, where the PRR4 gene is inserted into expression systems like *E. coli* or mammalian cell lines. This allows large-scale production of the protein with high purity and consistency. Recombinant variants often include tags (e.g., His-tag) for simplified purification via affinity chromatography. Post-translational modifications, such as glycosylation, may be preserved by using mammalian expression systems, ensuring functional similarity to the native protein.
Research on PRR4 has focused on its therapeutic potential, particularly in oral health. Studies suggest its antimicrobial properties against *Streptococcus mutans* and other oral pathogens, positioning it as a candidate for preventing dental caries or periodontal diseases. Additionally, PRR4’s role in wound healing and epithelial regeneration has spurred interest in developing biomaterials or topical formulations for mucosal repair.
Despite progress, challenges remain in optimizing stability and delivery mechanisms for clinical applications. Ongoing studies aim to elucidate PRR4’s interactions with host cells and microbial communities, as well as its potential in treating systemic inflammatory conditions. The development of recombinant PRR4 underscores its versatility as both a biological tool and a promising therapeutic agent in biomedicine.
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