| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TOP2b |
| Uniprot No | Q02880 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1411-1523aa |
| 氨基酸序列 | MAKSGGCGAGAGVGGGNGALTWVTLFDQNNAAKKEESETANKNDSSKKLSVERVYQKKTQLEHILLRPDTYIGSVEPLTQFMWVYDEDVGMNCREVTFVPGLYKIFDEILVNAADNKQRDKNMTCIKVSIDPESNIISIWNNGKGIPVVEHKVEKVYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETACKEYKHSFKQTWMNNMMKTSEAKIKHFDGEDYTCITFQPDLSKFKMEKLDKDIVALMTRRAYDLAGSCRGVKVMFNGKKLPVNGFRSYVDLYVKDKLDETGVALKVIHELANERWDVCLTLSEKGFQQISFVNSIATTKGGRHVDYVVDQVVGKLIEVVKKKNKAGVSVKPFQVKNHIWVFINCLIENPTFDSQTKENMTLQPKSFGSKCQLSEKFFKAASNCGIVESILNWVKFKAQTQLNKKCSSVKYSKIKGIPKLDDANDAGGKHSLECTLILTEGDSAKSLAVSGLGVIGRDRYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKSYDDAESLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLKHGFLEEFITPIVKASKNKQELSFYSIPEFDEWKKHIENQKAWKIKYYKGLGTSTAKEAKEYFADMERHRILFRYAGPEDDAAITLAFSKKKIDDRKEWLTNFMEDRRQRRLHGLPEQFLYGTATKHLTYNDFINKELILFSNSDNERSIPSLVDGFKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNINLLQPIGQFGTRLHGGKDAASPRYIFTMLSTLARLLFPAVDDNLLKFLYDD1411 to 1523 |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TOP2b重组蛋白的3篇代表性文献的简要列举：
1. **文献名称**：*"Recombinant human DNA topoisomerase IIβ: Production, purification, and characterization"*
**作者**：J.E. Deweese et al.
**摘要**：该研究报道了人源TOP2b重组蛋白在大肠杆菌中的高效表达与纯化方法，分析了其酶活性及对经典抑制剂(如依托泊苷)的敏感性，为体外药物筛选提供了工具。
2. **文献名称**：*"Functional analysis of TOP2B in neuronal differentiation using a recombinant protein system"*
**作者**：S. Li et al.
**摘要**：通过重组TOP2b蛋白，研究揭示了其在神经元分化过程中对染色质重塑和基因表达调控的关键作用，证明其酶活性缺失会导致分化障碍。
3. **文献名称**：*"Structural insights into TOP2B-DNA interaction by cryo-EM using recombinant human enzyme"*
**作者**：K. Roca et al.
**摘要**：利用冷冻电镜技术解析了重组TOP2b蛋白与DNA复合物的高分辨率结构，阐明了其催化循环中构象变化的分子机制。
*注：以上文献为示例性质，实际引用时建议通过PubMed或Web of Science核实最新研究。*
TOP2b (DNA topoisomerase II beta) is a crucial enzyme belonging to the type II topoisomerase family, which regulates DNA topology by introducing transient double-strand breaks during processes like replication, transcription, and chromosome segregation. Encoded by the TOP2B gene in humans, this 180-kDa protein is ubiquitously expressed but particularly abundant in post-mitotic cells such as neurons and cardiomyocytes, distinguishing it from its cell cycle-dependent isoform TOP2a.
Recombinant TOP2b proteins are typically produced using heterologous expression systems (e.g., baculovirus/insect cells or mammalian cell lines) to maintain proper post-translational modifications. The purified enzyme retains critical functional domains, including the ATPase domain, DNA-binding WHD (winged-helix domain), and catalytic tyrosine residue responsible for DNA cleavage-rejoining activity. Structural studies reveal a heart-shaped dimeric architecture enabling its DNA gate mechanism.
As a molecular target for chemotherapeutic agents like etoposide and doxorubicin, recombinant TOP2b facilitates drug mechanism studies and toxicity assessments. Its role extends beyond cancer biology, with emerging implications in neurodevelopment and neurodegenerative disorders. Researchers employ recombinant TOP2b to investigate its unique functional properties compared to TOP2a, particularly in non-proliferating cells where TOP2b dominates. However, challenges persist in maintaining enzymatic stability and preventing aggregation during purification. Recent advances in cryo-EM and single-molecule imaging techniques using recombinant TOP2b have provided unprecedented insights into its dynamic interaction with DNA and regulatory mechanisms, driving innovations in targeted therapies and precision medicine approaches.
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