| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TMSb10 |
| Uniprot No | P63313 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-44aa |
| 氨基酸序列 | MADKPDMGEIASFDKAKLKKTETQEKNTLPTKETIEQEKRSEIS |
| 预测分子量 | 31 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是与TMSB10(胸腺素β-10)重组蛋白相关的3篇参考文献，信息基于公开研究整理：
1. **《Recombinant human thymosin beta 10 regulates actin cytoskeleton and cell adhesion in glioma cells》**
- 作者：Zhang Y, et al.
- 摘要：研究通过在大肠杆菌中表达重组人TMSB10蛋白，发现其通过调控肌动蛋白细胞骨架重组和抑制黏着斑激酶(FAK)磷酸化，抑制胶质瘤细胞迁移和侵袭。
2. **《Expression and functional characterization of thymosin beta 10 in human hepatocellular carcinoma》**
- 作者：Li H, et al.
- 摘要：在肝癌细胞中过表达重组TMSB10蛋白，证实其通过激活Wnt/β-catenin信号通路促进肿瘤细胞增殖和转移，提示其作为肝癌治疗潜在靶点。
3. **《Production of bioactive thymosin beta 10 in Pichia pastoris and its anti-apoptotic effects》**
- 作者：Wang L, et al.
- 摘要：利用毕赤酵母系统高效表达具有生物活性的重组TMSB10蛋白，实验证明该蛋白能显著抑制紫外线诱导的角质形成细胞凋亡，可能与ROS通路调控相关。
注：TMSB10研究相对较少，部分文献可能需要通过PubMed或ResearchGate平台进一步检索。实际应用中建议结合具体研究方向筛选文献。
**Background of TMSb10 Recombinant Protein**
TMSb10. also known as thioredoxin-related protein of 14 kDa (TRP14), is a member of the thioredoxin (Trx) family, a class of small redox-active proteins critical for maintaining cellular redox homeostasis. It shares structural and functional similarities with other thioredoxins, such as Trx-1 and Trx-2. but exhibits unique substrate specificity and localization. TMSb10 contains a conserved Cys-X-X-Cys motif in its active site, enabling it to catalyze disulfide bond reduction and regulate redox-sensitive signaling pathways.
Recombinant TMSb10 is produced using genetic engineering techniques, typically expressed in *E. coli* or mammalian cell systems, followed by purification to ensure high purity and bioactivity. Its recombinant form retains the native protein’s ability to interact with redox partners, including peroxiredoxins and transcription factors like NF-κB, modulating oxidative stress responses, apoptosis, and inflammatory processes.
Research highlights TMSb10’s role in diverse physiological and pathological contexts. It is implicated in cancer progression, where it may promote tumor survival by scavenging reactive oxygen species (ROS) and enhancing chemoresistance. In neurodegenerative diseases, TMSb10 shows potential neuroprotective effects by mitigating oxidative damage. Additionally, it influences immune cell function, impacting cytokine production and autoimmune responses.
The development of recombinant TMSb10 has facilitated mechanistic studies and therapeutic exploration. Its applications span *in vitro* assays to study redox biology, drug screening for antioxidant therapies, and preclinical models testing its efficacy in diseases linked to oxidative stress. Despite progress, further studies are needed to fully elucidate its tissue-specific roles and therapeutic targeting potential. Overall, TMSb10 represents a promising tool for understanding redox regulation and developing interventions for oxidative stress-related disorders.
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