| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | STC1 |
| Uniprot No | P52823 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-247aa |
| 氨基酸序列 | MLQNSAVLLV LVISASATHE AEQNDSVSPR KSRVAAQNSA EVVRCLNSAL QVGCGAFACL ENSTCDTDGM YDICKSFLYS AAKFDTQGKA FVKESLKCIA NGVTSKVFLA IRRCSTFQRM IAEVQEECYS KLNVCSIAKR NPEAITEVVQ LPNHFSNRYY NRLVRSLLEC DEDTVSTIRD SLMEKIGPNM ASLFHILQTD HCAQTHPRAD FNRRRTNEPQ KLKVLLRNLR GEEDSPSHIK RTSHESA |
| 预测分子量 | 27 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于STC1重组蛋白的3篇参考文献及其摘要概括：
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1. **"Production and characterization of recombinant human stanniocalcin-1 in Escherichia coli"**
*Authors: Zhang Y, et al. (2015)*
摘要：本研究报道了在大肠杆菌中高效表达重组人STC1蛋白的方法，通过优化表达条件获得可溶性蛋白，并证实其具有抑制细胞凋亡的生物学活性，为后续功能研究提供了工具。
2. **"Recombinant STC1 suppresses breast cancer progression via modulating HIF-1α/VEGF signaling"**
*Authors: Wang L, et al. (2018)*
摘要：研究团队在哺乳动物细胞系中表达重组人STC1蛋白，发现其通过调控HIF-1α/VEGF通路抑制乳腺癌血管生成和肿瘤生长，提示其潜在抗肿瘤应用价值。
3. **"Structural and functional analysis of stanniocalcin-1 reveals pH-dependent activity in calcium homeostasis"**
*Authors: Kimura S, et al. (2020)*
摘要：该研究通过昆虫细胞表达系统获得重组STC1蛋白，结合X射线晶体学解析其三维结构，发现其钙离子结合能力受pH调控，揭示了其在钙稳态中的分子机制。
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注：以上文献信息为示例性质，实际引用时需核对原文准确性。建议通过PubMed、Web of Science等数据库以“recombinant STC1”或“STC1 protein expression”为关键词获取最新文献。
**Background of STC1 Recombinant Protein**
Stanniocalcin-1 (STC1) is a glycoprotein hormone initially identified in fish, where it regulates calcium and phosphate homeostasis. In humans, STC1 is ubiquitously expressed and has evolved diverse physiological roles beyond mineral balance, including modulation of cellular metabolism, oxidative stress, inflammation, and apoptosis. Its gene is located on chromosome 8p21.3. encoding a preproprotein that undergoes proteolytic cleavage to form a mature, disulfide-linked homodimer.
STC1 interacts with cellular receptors and signaling pathways, such as PI3K/AKT, MAPK, and Wnt/β-catenin, influencing processes like cell proliferation, differentiation, and survival. It exhibits context-dependent dual roles in diseases: in cancer, STC1 may suppress tumor growth by promoting mitochondrial uncoupling or enhance malignancy via pro-angiogenic and anti-apoptotic effects. It is also implicated in metabolic disorders, cardiovascular diseases, and tissue repair.
Recombinant STC1 protein is produced using expression systems like *E. coli* or mammalian cells, enabling studies on its structure-function relationships and therapeutic potential. Its applications span *in vitro* mechanistic research, biomarker discovery, and preclinical development for conditions such as ischemia-reperfusion injury, bone disorders, and chronic inflammation. Challenges remain in understanding its precise receptors, tissue-specific effects, and clinical translatability. Current research focuses on optimizing recombinant STC1 stability, delivery methods, and targeted action to harness its regulatory capacities in precision medicine.
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