| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RPN1 |
| Uniprot No | P04843 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 44-427aa |
| 氨基酸序列 | SHLAKVTAEVVLAHLGGGSTSRATSFLLALEPELEARLAHLGVQVKGEDEEENNLEVRETKIKGKSGRFFTVKLPVALDPGAKISVIVETVYTHVLHPYPTQITQSEKQFVVFEGNHYFYSPYPTKTQTMRVKLASRNVESYTKLGNPTRSEDLLDYGPFRDVPAYSQDTFKVHYENNSPFLTITSMTRVIEVSHWGNIAVEENVDLKHTGAVLKGPFSRYDYQRQPDSGISSIRSFKTILPAAAQDVYYRDEIGNVSTSHLLILDDSVEMEIRPRFPLFGGWKTHYIVGYNLPSYEYLYNLGDQYALKMRFVDHVFDEQVIDSLTVKIILPEGAKNIEIDSPYEISRAPDELHYTYLDTFGRPVIVAYKKNLVEQHIQDIVVH |
| 预测分子量 | 70.6kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RPN1重组蛋白的参考文献示例(注：以下为虚构示例，仅作格式参考，实际文献需通过学术数据库检索)：
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1. **"Structural characterization of recombinant RPN1 in the 26S proteasome regulatory complex"**
*Authors: Tanaka K., et al.*
**摘要**：本研究通过重组表达人源RPN1蛋白，结合冷冻电镜技术解析其三维结构，揭示了RPN1与蛋白酶体其他亚基相互作用的分子基础，为泛素依赖性底物识别提供结构依据。
2. **"Functional analysis of RPN1 in yeast proteasome assembly using recombinant variants"**
*Authors: Smith J., Weissman J.S.*
**摘要**：利用重组RPN1突变体，证明其在酵母蛋白酶体组装中的关键作用，并发现特定结构域对底物去泛素化活性的调控机制。
3. **"RPN1 recombinant protein as a therapeutic target in multiple myeloma"**
*Authors: Chen L., et al.*
**摘要**：通过体外重组RPN1蛋白筛选小分子抑制剂，发现其可特异性阻断蛋白酶体功能，为多发性骨髓瘤治疗提供潜在药物靶点。
4. **"High-yield expression and purification of recombinant RPN1 for biochemical assays"**
*Authors: Müller S., Dötsch V.*
**摘要**：优化大肠杆菌表达系统，实现重组RPN1蛋白的高效可溶性表达，并建立基于荧光标记的体外蛋白酶体活性检测体系。
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如需真实文献，建议通过 **PubMed** 或 **Google Scholar** 检索关键词：
`RPN1 recombinant protein structure/function/therapeutic application`。
RPN1 (Regulatory Particle Non-ATPase 1), also known as PSMD2. is a critical subunit of the 26S proteasome, a large multi-protein complex responsible for ubiquitin-dependent protein degradation in eukaryotic cells. As part of the 19S regulatory particle, RPN1 plays a pivotal role in recognizing polyubiquitinated substrates, facilitating their deubiquitination, and translocating them into the 20S catalytic core for proteolysis. This process is essential for maintaining cellular homeostasis, regulating cell cycle progression, and eliminating misfolded or damaged proteins.
Structurally, RPN1 contains multiple HEAT repeat domains that mediate interactions with other proteasome components, including ubiquitin receptors (e.g., PSMD4/RPN10) and deubiquitinating enzymes (e.g., USP14). It also serves as a docking platform for HSP70 chaperones, linking protein quality control pathways to proteasomal degradation. Studies have shown that RPN1 participates in diverse cellular processes, including DNA repair, immune response modulation, and stress adaptation.
Recombinant RPN1 protein is typically produced using heterologous expression systems (e.g., E. coli, insect, or mammalian cells) to study its biochemical properties, structural organization, and interaction networks. The purified protein often includes affinity tags (e.g., His-tag) for simplified isolation. Researchers employ RPN1 recombinant proteins in vitro to investigate proteasome assembly mechanisms, substrate recognition dynamics, and the effects of post-translational modifications (e.g., phosphorylation, SUMOylation) on proteasomal activity. Its recombinant form has become a valuable tool for drug discovery targeting proteasome dysfunction, particularly in neurodegenerative diseases and cancers where proteasomal impairment is implicated. Recent work also explores its potential as a diagnostic biomarker for certain malignancies.
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