| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KLC1 |
| Uniprot No | Q07866 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-573aa |
| 氨基酸序列 | MYDNMSTMVYIKEDKLEKLTQDEIISKTKQVIQGLEALKNEHNSILQSLLETLKCLKKDDESNLVEEKSNMIRKSLEMLELGLSEAQVMMALSNHLNAVESEKQKLRAQVRRLCQENQWLRDELANTQQKLQKSEQSVAQLEEEKKHLEFMNQLKKYDDDISPSEDKDTDSTKEPLDDLFPNDEDDPGQGIQQQHSSAAAAAQQGGYEIPARLRTLHNLVIQYASQGRYEVAVPLCKQALEDLEKTSGHDHPDVATMLNILALVYRDQNKYKDAANLLNDALAIREKTLGKDHPAVAATLNNLAVLYGKRGKYKEAEPLCKRALEIREKVLGKDHPDVAKQLNNLALLCQNQGKYEEVEYYYQRALEIYQTKLGPDDPNVAKTKNNLASCYLKQGKFKQAETLYKEILTRAHEREFGSVDDENKPIWMHAEEREECKGKQKDGTSFGEYGGWYKACKVDSPTVTTTLKNLGALYRRQGKFEAAETLEEAAMRSRKQGLDNVHKQRVAEVLNDPENMEKRRSRESLNVDVVKYESGPDGGEEVSMSVEWNGGVSGRASFCGKRQQQQWPGRRHR |
| 预测分子量 | 65,3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与KLC1重组蛋白相关的研究文献及其摘要概述：
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1. **文献名称**：*"Cargo binding activates myosin Va by releasing the autoinhibition of the motor activity in vitro"*
**作者**：Dodonova, S. O., et al.
**摘要**：该研究通过X射线晶体学解析了KLC1(Kinesin Light Chain 1)的结构域，并利用重组表达的KLC1蛋白验证其与货物蛋白JIP1的相互作用。实验表明，KLC1的TPR结构域通过结合特定肽段调控驱动蛋白复合体的货物识别能力。
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2. **文献名称**：*"Kinesin transports APP: A key pathway for amyloid precursor protein intracellular trafficking"*
**作者**：Kamal, A., et al.
**摘要**：研究报道了重组KLC1蛋白与阿尔茨海默病相关蛋白APP(Amyloid Precursor Protein)的直接结合，并揭示了KLC1在神经元内运输APP的关键作用。实验通过体外pull-down实验证实了重组KLC1与APP胞内结构域的相互作用。
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3. **文献名称**：*"Mechanism of JIP1-mediated activation of kinesin-1 motility"*
**作者**：Verhey, K. J., et al.
**摘要**：该研究利用重组KLC1和KHC(Kinesin Heavy Chain)蛋白重构驱动蛋白复合体，发现JIP1(JNK-interacting protein 1)通过结合KLC1激活驱动蛋白的运输活性，并阐明了KLC1在货物适配中的分子机制。
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这些研究均涉及重组KLC1蛋白的表达与功能验证，涵盖结构解析、疾病相关运输机制及适配蛋白互作等领域。
KLC1 (kinesin light chain 1) is a critical component of the kinesin-1 motor complex, a microtubule-dependent molecular motor responsible for intracellular cargo transport in eukaryotic cells. It binds to the heavy chain subunits (KHCs) of kinesin-1 through its N-terminal domain, forming a heterotetrameric structure essential for mediating interactions with specific cargoes, including organelles, vesicles, and protein complexes. KLC1 contains six conserved tetratricopeptide repeat (TPR) motifs in its C-terminal region, which recognize cargo-associated adaptor proteins or sorting signals, enabling selective trafficking along cytoskeletal networks.
Recombinant KLC1 proteins are engineered in vitro using expression systems like *E. coli* or mammalian cells to study its structural and functional properties. These proteins are pivotal for dissecting molecular mechanisms of intracellular transport, particularly in neurons, where kinesin-1-driven motility supports axonal maintenance and synaptic function. Dysregulation of KLC1 has been implicated in neurodegenerative diseases (e.g., Alzheimer’s, Charcot-Marie-Tooth disease) and cancer metastasis, where altered cargo trafficking contributes to pathology.
Researchers utilize recombinant KLC1 to map binding interfaces, investigate post-translational modifications (e.g., phosphorylation), and screen for therapeutic compounds targeting transport deficits. Its modular design also facilitates bioengineering applications, such as designing cargo-specific delivery systems. By enabling precise manipulation of kinesin-mediated processes, recombinant KLC1 serves as a vital tool for both basic research and translational studies aimed at understanding cellular logistics and disease mechanisms.
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