| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IBA1 |
| Uniprot No | P55008 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-147aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMSQTRDLQGGKAFGLLKAQQEERLDEINKQ FLDDPKYSSDEDLPSKLEGFKEKYMEFDLNGNGDIDIMSLKRMLEKLGVP KTHLELKKLIGEVSSGSGETFSYPDFLRMMLGKRSAILKMILMYEEKARE KEKPTGPPAKKAISELP |
| 预测分子量 | 19 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇涉及IBA1重组蛋白的文献摘要概览：
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1. **《Recombinant IBA1 Protein Expression and Purification in E. coli for Antibody Production》**
*Smith et al., 2018*
研究报道了在大肠杆菌中高效表达重组IBA1蛋白的方法，通过His标签纯化获得高纯度蛋白，并用于制备特异性抗体，验证了其在免疫组化中对小胶质细胞的标记效果。
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2. **《Structural Analysis of IBA1 Reveals Calcium-Dependent Actin-Binding Properties》**
*Yamamoto & Tanaka, 2020*
利用重组IBA1蛋白进行体外结构分析，阐明了其钙离子依赖性结合肌动蛋白的分子机制，揭示了IBA1在细胞骨架重塑中的关键作用。
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3. **《IBA1 Recombinant Protein as a Tool for Neuroinflammation Studies in vitro》**
*Lee et al., 2021*
通过哺乳动物细胞表达系统制备重组IBA1蛋白，证实其可激活小胶质细胞炎症通路(如NF-κB)，为神经退行性疾病模型提供了体外研究工具。
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注：以上文献名为虚拟示例，实际文献需通过PubMed、Web of Science等平台以关键词“IBA1 recombinant protein”检索。建议结合具体研究方向筛选实验方法学或应用类论文。
IBA1 (Ionized Calcium-Binding Adapter Molecule 1), also known as Allograft Inflammatory Factor 1 (AIF1), is a calcium-binding protein predominantly expressed in macrophages and microglia. First identified in activated microglia during brain injury, it plays a critical role in immune response regulation, cytoskeletal reorganization, and cell motility. The protein’s structure includes two EF-hand calcium-binding domains, enabling it to interact with actin filaments and modulate cellular activities in response to calcium signaling. Its expression is closely associated with neuroinflammation, neurodegenerative disorders, and immune-mediated diseases, making it a biomarker for microglial activation in conditions like Alzheimer’s disease, multiple sclerosis, and traumatic brain injury.
Recombinant IBA1 protein is produced using expression systems (e.g., E. coli or mammalian cells) to generate purified, biologically active forms of the protein for research. This engineered protein retains functional domains, allowing scientists to study its interactions with cytoskeletal components, signaling pathways, and inflammatory mediators in vitro. Applications include investigating microglial behavior in neurological disorders, screening anti-inflammatory compounds, and exploring its role in T-cell activation or transplant rejection. Its recombinant form is also used to develop antibodies for immunohistochemistry and flow cytometry, aiding in the visualization of immune cell dynamics in disease models. By providing a standardized tool, recombinant IBA1 enhances reproducibility in studies linking microglial dysfunction to pathological processes.
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