| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TOP1 |
| Uniprot No | P11387 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 190-765aa |
| 氨基酸序列 | NKKKKPKKEEEQKWKWWEEERYPEGIKWKFLEHKGPVFAPPYEPLPENVKFYYDGKVMKLSPKAEEVATFFAKMLDHEYTTKEIFRKNFFKDWRKEMTNEEKNIITNLSKCDFTQMSQYFKAQTEARKQMSKEEKLKIKEENEKLLKEYGFCIMDNHKERIANFKIEPPGLFRGRGNHPKMGMLKRRIMPEDIIINCSKDAKVPSPPPGHKWKEVRHDNKVTWLVSWTENIQGSIKYIMLNPSSRIKGEKDWQKYETARRLKKCVDKIRNQYREDWKSKEMKVRQRAVALYFIDKLALRAGNEKEEGETADTVGCCSLRVEHINLHPELDGQEYVVEFDFLGKDSIRYYNKVPVEKRVFKNLQLFMENKQPEDDLFDRLNTGILNKHLQDLMEGLTAKVFRTYNASITLQQQLKELTAPDENIPAKILSYNRANRAVAILCNHQRAPPKTFEKSMMNLQTKIDAKKEQLADARRDLKSAKADAKVMKDAKTKKVVESKKKAVQRLEEQLMKLEVQATDREENKQIALGTSKLNYLDPRITVAWCKKWGVPIEKIYNKTQREKFAWAIDMADEDYEF |
| 预测分子量 | 72.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于TOP1(拓扑异构酶I)重组蛋白的经典文献示例，供参考：
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1. **文献名称**：*"Human topoisomerase I: Structure, functions, and therapeutic potential"*
**作者**：Pommier, Y.
**摘要**：综述了人源TOP1的结构与功能，重点讨论了重组TOP1在抗癌药物(如伊立替康)研究中的作用，及其通过稳定DNA断裂复合物诱导癌细胞凋亡的机制。
2. **文献名称**：*"Expression and purification of recombinant human topoisomerase I for structural studies"*
**作者**：Staker, B.L. et al.
**摘要**：报道了重组人TOP1在大肠杆菌中的高效表达与纯化方法，并通过X射线晶体学解析其与DNA复合物的三维结构，为药物设计提供依据。
3. **文献名称**：*"Functional analysis of recombinant topoisomerase I from Plasmodium falciparum"*
**作者**：Bhattacharya, S. et al.
**摘要**：研究了恶性疟原虫来源的重组TOP1酶活性，发现其与人类TOP1的差异性，为开发抗疟疾靶向药物奠定基础。
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**备注**：以上文献为示例，实际引用时建议通过PubMed或Web of Science检索最新研究，并核对原文信息。
**Background of TOP1 Recombinant Protein**
Topoisomerase I (TOP1) is a critical enzyme involved in DNA replication, transcription, and repair by transiently cleaving and religating one strand of the DNA duplex to resolve topological stress, such as supercoiling. Dysregulation of TOP1 activity is linked to genomic instability and diseases, including cancer. Recombinant TOP1 protein, produced via genetic engineering in heterologous expression systems (e.g., *E. coli* or mammalian cells), retains the enzymatic functions of native TOP1 and serves as a vital tool for biochemical and pharmacological studies.
The development of recombinant TOP1 emerged from the need to study its role in DNA metabolism and its interaction with therapeutic agents. For instance, TOP1 is a primary target of camptothecin-derived anticancer drugs (e.g., irinotecan, topotecan), which stabilize TOP1-DNA cleavage complexes, leading to lethal DNA damage in rapidly dividing cells. Recombinant TOP1 enables *in vitro* assays to evaluate drug efficacy, resistance mechanisms, and structure-function relationships.
Structurally, recombinant TOP1 typically includes conserved domains essential for catalysis, such as the N-terminal domain for nuclear localization, the core domain for DNA binding, and the C-terminal catalytic domain. Advanced purification techniques (e.g., affinity chromatography) ensure high purity and activity. Its applications extend to cancer research, drug discovery, and mechanistic studies of DNA repair pathways.
By providing a controllable and scalable source of functional TOP1. recombinant protein technology has significantly advanced our understanding of topoisomerase biology and anticancer drug development, bridging molecular insights with therapeutic innovation.
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