| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FAPa |
| Uniprot No | Q12884 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 26-760aa |
| 氨基酸序列 | LRPSRVHNSEENTMRALTLKDILNGTFSYKTFFPNWISGQEYLHQSADNNIVLYNIETGQSYTILSNRTMKSVNASNYGLSPDRQFVYLESDYSKLWRYSYTATYYIYDLSNGEFVRGNELPRPIQYLCWSPVGSKLAYVYQNNIYLKQRPGDPPFQITFNGRENKIFNGIPDWVYEEEMLATKYALWWSPNGKFLAYAEFNDTDIPVIAYSYYGDEQYPRTINIPYPKAGAKNPVVRIFIIDTTYPAYVGPQEVPVPAMIASSDYYFSWLTWVTDERVCLQWLKRVQNVSVLSICDFREDWQTWDCPKTQEHIEESRTGWAGGFFVSTPVFSYDAISYYKIFSDKDGYKHIHYIKDTVENAIQITSGKWEAINIFRVTQDSLFYSSNEFEEYPGRRNIYRISIGSYPPSKKCVTCHLRKERCQYYTASFSDYAKYYALVCYGPGIPISTLHDGRTDQEIKILEENKELENALKNIQLPKEEIKKLEVDEITLWYKMILPPQFDRSKKYPLLIQVYGGPCSQSVRSVFAVNWISYLASKEGMVIALVDGRGTAFQGDKLLYAVYRKLGVYEVEDQITAVRKFIEMGFIDEKRIAIWGWSYGGYVSSLALASGTGLFKCGIAVAPVSSWEYYASVYTERFMGLPTKDDNLEHYKNSTVMARAEYFRNVDYLLIHGTADDNVHFQNSAQIAKALVNAQVDFQAMWYSDQNHGLSGLSTNHLYTHMTHFLKQCFSLSD |
| 预测分子量 | 112.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于FAPα重组蛋白研究的代表性文献，涵盖结构、功能及治疗应用方向：
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1. **文献名称**：*Structural basis of proline-specific exopeptidase activity observed in human fibroblast activation protein α*
**作者**：Aertgeerts, K. et al.
**摘要**：该研究解析了人源FAPα的晶体结构，揭示了其脯氨酸特异性外肽酶活性的分子机制，并发现其与DPP-IV的结构差异，为开发选择性抑制剂提供依据。
2. **文献名称**：*Recombinant expression and functional characterization of fibroblast activation protein α in tumor microenvironment remodeling*
**作者**：Huang, Y. et al.
**摘要**：通过哺乳动物表达系统成功制备重组FAPα蛋白，证实其在体外可降解细胞外基质成分(如胶原)，促进肿瘤细胞侵袭，提示其参与微环境重塑的病理过程。
3. **文献名称**：*Targeting FAPα-expressing stromal cells with a prodrug-conjugated recombinant antibody-enzyme fusion protein*
**作者**：Loktev, A. et al.
**摘要**：构建了抗FAPα抗体-重组酶融合蛋白，在小鼠模型中验证了其靶向递送前药的能力，显著抑制肿瘤生长，展示了FAPα作为治疗靶点的应用潜力。
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*注：以上为示例性文献，实际研究中建议通过PubMed等数据库获取最新具体文献(检索关键词：Fibroblast Activation Protein alpha, recombinant expression, cancer-associated fibroblasts)。部分研究可能涉及FAPα与DPP-IV的双酶活性比较或抗体工程应用方向。*
**Background of FAPα Recombinant Protein**
Fibroblast Activation Protein alpha (FAPα) is a type II transmembrane serine protease belonging to the dipeptidyl peptidase 4 (DPP4) family. It exists as a homodimer and exhibits both dipeptidyl peptidase and endopeptidase activities, enabling cleavage of substrates like collagen and α2-antiplasmin. FAPα is minimally expressed in healthy adult tissues but is markedly upregulated in activated fibroblasts, particularly cancer-associated fibroblasts (CAFs), during tissue remodeling, wound healing, or pathological states such as fibrosis and solid tumors.
In cancer, FAPα+ CAFs are key players in tumor progression, modulating extracellular matrix (ECM) remodeling, immunosuppression, angiogenesis, and metastasis. Its overexpression correlates with poor prognosis in cancers like pancreatic, breast, and colorectal carcinomas, making it a potential therapeutic target.
Recombinant FAPα proteins are engineered using heterologous expression systems (e.g., mammalian, insect, or bacterial cells) to produce soluble, catalytically active forms for research and drug development. These proteins retain enzymatic activity and structural features of native FAPα, enabling studies on substrate specificity, inhibitor screening, and mechanistic insights into tumor-stroma interactions.
Recent applications include developing FAPα-targeted therapies, such as small-molecule inhibitors, immunotherapies (e.g., CAR-T cells), and radiopharmaceuticals for tumor imaging. However, challenges remain in understanding its dual roles in homeostasis versus disease and optimizing selective targeting to avoid off-tissue effects. Ongoing research continues to explore FAPα's biology and translational potential in oncology and fibrotic disorders.
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