| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MST |
| Uniprot No | P25325 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-297aa |
| 氨基酸序列 | ASPQLCRAL VSAQWVAEAL RAPRAGQPLQ LLDASWYLPK LGRDARREFE ERHIPGAAFF DIDQCSDRTS PYDHMLPGAE HFAEYAGRLG VGAATHVVIY DASDQGLYSA PRVWWMFRAF GHHAVSLLDG GLRHWLRQNL PLSSGKSQPA PAEFRAQLDP AFIKTYEDIK ENLESRRFQV VDSRATGRFR GTEPEPRDGI EPGHIPGTVN IPFTDFLSQE GLEKSPEEIR HLFQEKKVDL SKPLVATCGS GVTACHVALG AYLCGKPDVP IYDGSWVEWY MRARPEDVIS EGRGKTH |
| 预测分子量 | 33,1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MST重组蛋白的3篇代表性文献摘要示例(注：文献为虚构示例，仅作格式参考)：
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1. **文献名称**：*Expression and Functional Analysis of Recombinant MST1 Protein in Mammalian Cells*
**作者**：Wang X, et al.
**摘要**：研究通过哺乳动物表达系统成功制备重组人MST1蛋白，并验证其激酶活性及在Hippo信号通路中对细胞凋亡的调控作用。
2. **文献名称**：*Crystallographic Study of Recombinant MST2 for Structural Activation Mechanism*
**作者**：Tanaka R, et al.
**摘要**：利用重组MST2蛋白进行X射线晶体结构解析，揭示其自抑制构象及RASSF5蛋白结合后的激活机制。
3. **文献名称**：*Therapeutic Efficacy of Recombinant MST1/2 Fusion Protein in Inflammatory Disease Models*
**作者**：Chen L, et al.
**摘要**：开发新型MST1/2重组融合蛋白，证明其通过抑制NF-κB通路减轻小鼠结肠炎模型的炎症反应和组织损伤。
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如需真实文献，建议通过PubMed或Google Scholar搜索关键词“MST recombinant protein”“MST1/2 kinase recombinant”等获取。
**Background of MST Recombinant Protein**
MST (Macrophage Stimulating Protein), also known as MSP or hepatocyte growth factor-like protein, is a plasminogen-related growth factor involved in regulating immune responses, tissue repair, and cellular homeostasis. It is primarily produced by hepatocytes and circulates as an inactive precursor (pro-MSP) that requires proteolytic cleavage for activation. The mature MST protein is a heterodimer composed of α and β chains linked by disulfide bonds, with structural homology to hepatocyte growth factor (HGF).
Biologically, MST binds to the RON (Receptor d’Origine Nantais) tyrosine kinase receptor, predominantly expressed on macrophages, epithelial cells, and certain cancer cells. Activation of RON triggers downstream signaling pathways, including MAPK, PI3K-Akt, and NF-κB, which regulate processes such as cell migration, inflammation, and survival. MST-RON signaling plays dual roles: it promotes tissue repair by modulating macrophage phagocytosis and resolving inflammation, yet aberrant activation is linked to pathological conditions, including cancer progression, fibrosis, and chronic inflammatory diseases.
Recombinant MST proteins are engineered using expression systems (e.g., mammalian CHO cells) to ensure proper post-translational modifications and bioactivity. These proteins are critical tools for studying RON-mediated mechanisms and developing therapeutic strategies. For instance, MST-based inhibitors or RON-targeted antibodies are explored to block oncogenic signaling, while recombinant MST itself may aid in regenerative therapies by enhancing tissue repair.
Overall, MST recombinant proteins bridge fundamental research and clinical applications, offering insights into cellular signaling dynamics and potential interventions for diseases driven by dysregulated MST-RON pathways.
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