| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TSPAN3 |
| Uniprot No | O60637 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-253aa |
| 氨基酸序列 | MGQCGITSSKTVLVFLNLIFWGAAGILCYVGAYVFITYDDYDHFFEDVYT LIPAVVIIAVGALLFIIGLIGCCATIRESRCGLATFVIILLLVFVTEVVV VVLGYVYRAKVENEVDRSIQKVYKTYNGTNPDAASRAIDYVQRQLHCCGI HNYSDWENTDWFKETKNQSVPLSCCRETASNCNGSLAHPSDLYAEGCEAL VVKKLQEIMMHVIWAALAFAAIQLLGMLCACIVLCRRSRDPAYELLITGG TYA |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于TSPAN3重组蛋白的示例文献摘要(注：以下内容为假设性示例，实际文献需通过学术数据库查询)：
1. **文献名称**：*"Recombinant expression and functional characterization of human TSPAN3 in cancer cell migration"*
**作者**：Li, X. et al.
**摘要**：本研究通过哺乳动物表达系统成功制备了重组人源TSPAN3蛋白，并验证其促进乳腺癌细胞迁移的功能。实验表明，TSPAN3通过与整合素β1相互作用激活下游信号通路。
2. **文献名称**：*"Structural analysis of TSPAN3 extracellular domain using recombinant protein expressed in HEK293 cells"*
**作者**：Yamamoto, S. et al.
**摘要**：作者利用HEK293细胞表达并纯化TSPAN3胞外结构域重组蛋白，通过X射线晶体学解析其三维结构，揭示了其与其他四次跨膜蛋白相互作用的潜在位点。
3. **文献名称**：*"Development of a bacterial expression system for high-yield production of bioactive TSPAN3"*
**作者**：Chen, J. et al.
**摘要**：该研究优化了大肠杆菌表达体系，实现TSPAN3重组蛋白的可溶性表达与纯化，并证实其体外结合肝素的能力，为后续药物筛选提供工具。
4. **文献名称**：*"TSPAN3 modulates amyloid precursor protein processing via recombinant protein-mediated assay"*
**作者**：Wang, H. et al.
**摘要**：通过重组TSPAN3蛋白的体外实验，发现其能够与γ-分泌酶复合物结合，影响淀粉样前体蛋白(APP)的切割过程，提示其在阿尔茨海默病中的潜在作用。
(注：以上为模拟内容，实际研究请参考PubMed、Web of Science等数据库。)
Tspan3. a member of the tetraspanin protein family, is a cell membrane-associated protein characterized by four transmembrane domains that form distinct structural loops and interact with diverse partner molecules. These interactions enable tetraspanins like Tspan3 to organize membrane microdomains ("tetraspanin webs") that regulate cellular processes such as adhesion, signaling, and intracellular trafficking. While Tspan3's precise biological roles remain under investigation, studies suggest its involvement in neuronal development, immune responses, and cancer progression. For instance, Tspan3 has been linked to modulating neurotransmitter receptor trafficking in neurons and enhancing metastatic potential in certain cancers by promoting cell migration.
Recombinant Tspan3 protein is engineered for research and therapeutic applications. Produced via heterologous expression systems (e.g., bacterial, mammalian, or insect cells), the recombinant form typically retains key structural features like conserved cysteine residues in the large extracellular loop, which are critical for protein folding and interaction capabilities. Researchers employ affinity tags (e.g., His-tag, FLAG) for purification and detection. Current applications include:
1. **Mechanistic studies**: Unraveling Tspan3's role in membrane protein organization and signaling pathways
2. **Antibody development**: Generating specific antibodies for diagnostic or therapeutic use
3. **Drug discovery**: Screening molecules that disrupt pathogenic Tspan3 interactions
4. **Structural analysis**: Resolving 3D conformations to guide targeted interventions
Challenges persist in maintaining native post-translational modifications during recombinant production and deciphering context-dependent functions across tissues. Emerging evidence of Tspan3's overexpression in gliomas and breast cancers has intensified interest in its potential as a biomarker or therapeutic target. Ongoing research aims to clarify its tissue-specific interactome and validate preclinical models using recombinant protein tools.
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