| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TRPM1 |
| Uniprot No | Q7Z4N2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1127-1603aa |
| 氨基酸序列 | RCRKKREGDQEERDRGLKLFLSDEELKRLHEFEEQCVQEHFREKEDEQQSSSDERIRVTSERVENMSMRLEEINERETFMKTSLQTVDLRLAQLEELSNRMVNALENLAGIDRSDLIQARSRASSECEATYLLRQSSINSADGYSLYRYHFNGEELLFEDTSLSTSPGTGVRKKTCSFRIKEEKDVKTHLVPECQNSLHLSLGTSTSATPDGSHLAVDDLKNAEESKLGPDIGISKEDDERQTDSKKEETISPSLNKTDVIHGQDKSDVQNTQLTVETTNIEGTISYPLEETKITRYFPDETINACKTMKSRSFVYSRGRKLVGGVNQDVEYSSITDQQLTTEWQCQVQKITRSHSTDIPYIVSEAAVQAEHKEQFADMQDEHHVAEAIPRIPRLSLTITDRNGMENLLSVKPDQTLGFPSLRSKSLHGHPRNVKSIQGKLDRSGHASSVSSLVIVSGMTAEEKKVKKEKASTETEC |
| 预测分子量 | 61.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于TRPM1重组蛋白的研究文献概览(注：文献信息为模拟示例，实际引用请核实原文)：
1. **文献名称**: *"TRPM1 is a component of the retinal pathway modulating synaptic transmission"*
**作者**: Koike C, et al.
**摘要**: 研究利用重组TRPM1蛋白揭示了其在视网膜双极细胞中的表达及功能，证明TRPM1通过调控钙离子内流参与光信号传递，其突变可能导致先天性夜盲症。
2. **文献名称**: *"Recombinant TRPM1 expression in HEK293 cells reveals cation channel properties"*
**作者**: Oancea E, et al.
**摘要**: 通过HEK293细胞表达重组TRPM1蛋白，证实其形成非选择性阳离子通道，并发现其活性受G蛋白偶联受体调控，为研究TRPM1在黑色素细胞中的信号机制提供模型。
3. **文献名称**: *"Structural insights into TRPM1 channel gating by cryo-EM"*
**作者**: Duan J, et al.
**摘要**: 采用冷冻电镜解析重组TRPM1蛋白的高分辨率结构，揭示其门控机制及与疾病相关突变的构效关系，为靶向药物设计奠定基础。
(提示：实际文献检索建议通过PubMed或Google Scholar以关键词“TRPM1 recombinant protein”查询近年研究。)
TRPM1 (Transient Receptor Potential Melastatin 1) is a calcium-permeable ion channel belonging to the TRP superfamily, specifically the melastatin subfamily. Initially identified for its reduced expression in metastatic melanoma cells, TRPM1 is now recognized for its dual role in melanocyte biology and retinal signaling. In melanocytes, it influences pigmentation and cellular differentiation, though its exact mechanism remains under investigation. In the retina, TRPM1 is critically expressed in ON-bipolar cells, where it mediates signal transduction in the visual pathway. Here, it functions as a key component of the depolarizing response to light, activated downstream of metabotropic glutamate receptor 6 (mGluR6) signaling. Mutations in the TRPM1 gene are linked to congenital stationary night blindness (CSNB), highlighting its essential role in vision.
Recombinant TRPM1 protein is engineered for in vitro studies to dissect its structural and functional properties. Produced using heterologous expression systems (e.g., HEK293 or insect cells), the purified protein enables researchers to study channel gating mechanisms, ion selectivity (Ca²⁺, Na⁺), and interactions with regulatory proteins like TRPM1-associated vesicles or mGluR6 complex components. Its six transmembrane domains and cytoplasmic N-/C-termini are often analyzed via cryo-EM or electrophysiology (patch-clamp) to map activation sites or disease-associated mutations. Recombinant TRPM1 also aids drug screening for retinal disorders or melanoma therapies, offering insights into channel modulation. Despite challenges in maintaining native conformation during purification, this tool remains vital for advancing TRP channel biology and translational research.
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